Fig. 4

ELF4 deficiency aggravated OS and ERS. Serum SOD (A), CAT (B), and GSH-PX (C) levels in WT and ELF4^−/− mice. D In kidney tissues of WT and ELF4^−/− mice, Nrf2, HO-1, and NQO-1 protein levels were surveyed by way of western blot. E GRP78, CHOP, and caspase-12 protein levels were detected by way of western blot in kidney tissues of WT and ELF4^−/− mice. F Flow cytometry was carried out to assess ROS levels in HK-2 cells. The levels of OS related proteins (Nrf2, HO-1, and NQO-1; G), ER stress related proteins (GRP78, CHOP, and caspase-12; H) were surveyed using western blot in HK-2 cells. Mouse n = 5. Cell n = 3. * p < 0.05 WT sham or control group, # p < 0.05 ELF4^−/− sham, WT I/R, or OGD/R group