Fig. 8

Recovered peptides for JAK3 and CDKN1B from HPLC/MS/MS analysis. a Peptides sequences of JAK3 from human and bovine species were aligned to evaluate similarities and conserved modifications associated with JAK3 activation. Peptides positions within the JAK3 sequence are indicated and phosphorylated residues are shown in blue. In particular, phosphorylation of the two Tyrosine (Y) residues at locations 980–981 (peptide #29.1 in the figure) of JAK3 amino acid sequence in human were retrieved in this study. Similarly, different peptides with residues known to be phosphorylated in the human sequence were also found in this study meaning that these amino acid residues were also phosphorylated in bovine granulosa cells following JAK3 activation with FSH. b For CDKN1B, the two highlighted Threonine (T) residues at locations 187 and 198 within peptide #7 are an indication of phosphorylation status of CDKN1B and are related to CDKN1B inactivation. Phosphorylated of this peptide was more abundant following FSH treatment (and JAK3 activation) as shown in Fig. 6d, while JNX treatment (and JAK3 inhibition) resulted in the reduction in CDKN1B phosphorylation