Fig. 7

Effects of FSH and JANEX-1 treatments on MAPK8IP3 total abundance and peptides modifications in granulosa cells. HPLC/MS–MS was used to analyze and quantify total abundances of various recovered peptides for MAPK8IP3 in GC samples following treatments with FSH or JNX. a MAPK8IP3 total abundance did not change following FSH treatment but JNX treatment significantly decreased MAPK8IP3 abundance. b Ratio of fold change in MAPK8IP3 abundance in FSH-treated cells over the control and in JNX-treated cells over the control are compared and showed MAPK8IP3 as significantly more abundant following FSH treatment. c-d Peptides abundance and phosphorylation at specific amino acid residues. The abundances of two MAPK8IP3 phosphorylated peptides were quantified and evaluated following FSH or JNX treatments. Peptide numbers and positions in the MAPK8IP3 protein sequence are shown. Phosphorylated residues are shown in red. CTRL, control; FSH, Follicle-stimulating hormone; JNX, JANEX-1. *, p ≤ 0.05; **, p ≤ 0.01 (ANOVA, Tukey–Kramer multiple comparison); ns, not significant