Fig. 6

Effects of FSH and JANEX-1 treatments on CDKN1B total abundance and peptides modifications in granulosa cells. HPLC/MS–MS was used to analyze and quantify total abundances of various recovered peptides for CDKN1B in GC samples following treatments with FSH or JNX. a CDKN1B total abundance tended to decrease following FSH treatment (P = 0.075) while CDKN1B was significantly more abundant in JNX-treated cells. b Ratio of fold change in CDKN1B abundance in FSH-treated cells over the control and in JNX-treated cells over the control are compared and showed CDKN1B more abundant following JNX treatment. c-d Peptides abundance and phosphorylation at specific amino acid residues. The abundances of two CDKN1B phosphorylated peptides were quantified and evaluated following FSH or JNX treatments. Peptide numbers and positions in the CDKN1B protein sequence are shown. Phosphorylated residues are shown in red. CTRL, control; FSH, Follicle-stimulating hormone; JNX, JANEX-1. *, p ≤ 0.05; **, p ≤ 0.01 (ANOVA, Tukey–Kramer multiple comparison), ns Not significant