Fig. 5

Effects of FSH and JANEX-1 treatments on JAK3 total abundance and peptides modifications in granulosa cells. HPLC/MS–MS was used to analyze and quantify total abundances of various recovered peptides for JAK3 in GC samples following treatments with FSH or JNX. a JAK3 total abundance slightly increased following FSH treatment but not significantly while JNX treatment significantly decreased JAK3 abundance. b Ratio of fold change in JAK3 abundance in FSH-treated cells over the control and in JNX-treated cells over the control are compared and showed JAK3 as significantly more abundant following FSH treatment (p = 0.05). c-f Peptides abundance and phosphorylation at specific amino acid residues. The abundances of various JAK3 phosphorylated peptides were quantified and evaluated following FSH or JNX treatments. Peptide numbers and positions in the JAK3 protein sequence are shown. Phosphorylated residues are shown in red. CTRL, control; FSH, Follicle-stimulating hormone; JNX, JANEX-1. *, p ≤ 0.05 (ANOVA, Tukey–Kramer multiple comparison), ns Not significant