Fig. 3
Effect of JAK3 inhibition on steroidogenesis markers (CYP11A1 and CYP19A1) in granulosa cells. Total RNA was extracted from cultured GC treated with or without FSH or JANEX-1 and analyzed by RT-PCR for the expression of steroidogenic enzymes cytochrome P450 family 11 subfamily A member 1 (CYP11A1) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) normalized against the house keeping gene RPL19 as reference. The results show that FSH significantly upregulated CYP11A1 in GC (a); however, CYP19A1 (b) did change following FSH treatment. In addition, JAK3 inhibition with JNX resulted in a significant decrease of both CYP11A1 and CYP19A1. FSH treatment following JANEX-1 significantly increased CYP19A1 expression but not CYP11A1. CTRL, control; FSH, Follicle-stimulating hormone; JNX, JANEX-1. RT-qPCR data are presented as gene abundance relative to RPL19 using the 2−ΔΔCt method. *, p ≤ 0.05, **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001 (ANOVA, Tukey–Kramer multiple comparison), ns Not significant