Fig. 2

Regulation of JAK3 mRNA expression in vitro. Total RNA was extracted from cultured GC treated with or without FSH or JNX and analyzed by RT-PCR for the expression of proliferation markers Cyclin-D2 (CCND2) and Proliferating cell nuclear antigen (PCNA) as well as JAK3 relative to RPL19 as reference gene. FSH stimulated both CCND2 (a) and PCNA (b) mRNA expression levels in GC while JANEX-1 significantly decreased both of these proliferation markers after 24 h. In addition, addition of FSH following JNX treatment significantly increased expression of both CCND2 and PCNA as compared to JNX. RT-qPCR analysis showed that JAK3 mRNA expression in GC (c) significantly decreased after JNX treatment as compared to the control and FSH-treated cells. FSH treatment alone did not stimulate JAK3 expression as compared to control group; however, FSH treatment significantly increased JAK3 expression following JNX treatment. CTRL, control; FSH, Follicle-stimulating hormone; JNX, JANEX-1 RT-qPCR data are presented as gene abundance using the 2^−ΔΔCt method. *, p ≤ 0.05, **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001 (ANOVA, Tukey–Kramer multiple comparison); ns Not significant