Fig. 1

Regulation of JAK family members during follicular development. mRNA expression and regulation of JAK family members were analyzed in GC of bovine follicles at different stages using RT-qPCR. Total RNA was extracted from GC of small follicles (SF; N = 3), dominant follicles (DF; N = 4), ovulatory follicles (OF; N = 4), and corpus luteum at day 5 (CL; N = 4) as described in materials and methods and target genes were analyzed (JAK1, JAK2, JAK3, TYK2) relative to RPL19 as reference gene. JAK3 mRNA expression was strongest in DF and SF and was significantly decreased in OF and CL (p ≤ 0.05) while other JAK members were differentially expressed in different stages of follicular development and CL. This result suggests a different regulation of JAK family in granulosa cells at different stages of follicular development and CL. RT-qPCR data are presented as gene abundance using the 2^−ΔΔCt method. *, p ≤ 0.05, **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001 (ANOVA, Tukey–Kramer multiple comparison), ns Not significant