Fig. 3

The 8-OHdG expression in the SV and quantification of the mtDNA CDs. (A) Representative images showing the expression and localization of 8-OHdG in the SV of mice in the control, D-gal-L, and D-gal-H groups. Scale bars = 25 μm. (Red: 8-OhdG; blue: DAPI). (B) The protein expression of 8-OHdG in the SV of the D-gal-L group was significantly higher than that of the control group mice but lower compared to that of the D-gal-H group. (C) The mtDNA CD level in the cochleae of mice in the D-gal-L group was significantly higher than that in the mice of the control group but lower compared to that of the D-gal-H group. Data are expressed as the mean ± SD of 6 mice per group. **, P < 0.01 vs. the control group; ^, P < 0.05 vs. the D-gal-L group; ^^, P < 0.01 vs. the D-gal-L group