Table 1 Primers for RT-PCR
From: microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells
Name | Sequence (5′-3′) | miRbase/Gene ID |
|---|---|---|
miR-326-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGGAG | MIMAT0000756 |
miR-326-F | CGCCTCTGGGCCCTTC | |
miR-126-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGCGTA | MIMAT0000444 |
miR-126-5p-F | GCGCGCATTATTACTTTTGG | |
miR-338-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAACAA | MIMAT0000763 |
miR-338-3p-F | CGCGTCCAGCATCAGTGATT | |
miR-599-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTTTGA | MIMAT0003267 |
miR-599-F | CGCGCGGTTGTGTCAGTTTA | |
miR-548Â m-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAAAA | MIMAT0005917 |
miR-548Â m-F | CGCGCAAAGGTATTTGTGG | |
miR-16-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGCCAA | MIMAT0000069 |
miR-16-5p-F | CGCGTAGCAGCACGTAAATA | |
miR-214-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCACAG | MIMAT0004564 |
miR-214-5p-F | CGCGTGCCTGTCTACACTTG | |
miR-30c-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCTGAG | MIMAT0000244 |
miR-30c-5p-F | GCGCGTGTAAACATCCTACACT | |
miR-221-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAAACC | MIMAT0000278 |
miR-221-3p-F | CGCGAGCTACATTGTCTGCTG | |
miR-4763-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCGCC | MIMAT0019913 |
miR-4763-3p-F | GCAGGGGCTGGTGCTG | |
Bcl-2-F | ATCGCCCTGTGGATGACTGAGT | 596 |
Bcl-2-R | GCCAGGAGAAATCAAACAGAGGC | |
Bax-F | TCAGGATGCGTCCACCAAGAAG | 581 |
Bax-R | TGTGTCCACGGCGGCAATCATC | |
U6-F | CTCGCTTCGGCAGCACA | 26,827 |
U6-R | AACGCTTCACGAATTTGCGT | |
public reverse primer for miRNA | AGTGCAGGGTCCGAGGTATT | Â |
18Â s-F | ACCCGTTGAACCCCATTCGTGA | 100,008,588 |
18Â s-R | GCCTCACTAAACCATCCAATCGG | |
F Forward primer, R Reverse primer, RT Specific primer of reverse transcription | ||