Fig. 4
Electrophysiological measurements of WT and CF mouse IEC monolayers. a Representative traces of electrophysiological measurements of WT and CF mouse IEC monolayers in Ussing chambers. Amiloride (Amil), Forskolin + IBMX (Fsk/IBMX), UTP and GlyH-101 were added sequentially. b Mean Forskolin/IBMX, UTP and GlyH101 responses measured in Ussing chambers. Electrophysiological measurements were performed on IEC monolayers derived from 2 individual WT mice and 3 individual ∆F508 CFTR mice. c Measured bioelectric characteristics of WT and CF IEC monolayer mouse small intestinal cultures. Basal potential difference (mV), transepithelial resistance (Ω x cm2), and current (μA/cm2) are reported. Data represented at mean ± SEM based on cultures. *p < 0.05