Fig. 1From: Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cellsEfficient engagement of either apoptosis or CICD in melanoma cells. a. In vitro system of triggering either apoptosis or CICD in melanoma cells based on doxycycline-mediated rapid expression of the pro-apoptotic BAX protein. b Apoptosis (1 μg/ml DOX-treated cells) or CICD (DOX and Q-VD-OPh treated cells) was engaged in WM115 for 24 h followed by immunoblotting for BAX, caspase-3, PARP1 and actin as loading control. c WM115 cells were treated as in (b) and cell viability was measured by SYTOX Green exclusion in an Incucyte Imager. A representative experiment is shown. d Efficacy of CRISPR/Cas9-mediated APAF-1 KO in WM115 cells. e Validation of apoptosis and CICD induction in APAF-1 KO WM115 cells. DOX treatment and immunoblotting was done as described in (b). f Representative SYTOX Green positive staining for either apoptotic or cells undergoing CICD at 24 h. g Cell death kinetics for apoptosis and CICD triggered in the context of APAF-1 KO. A representative experiment is shown. h-i. Immunofluorescence representative images (h) for the release of cytochrome c and quantification (i)Back to article page