Fig. 4
From: mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells
Western blot analysis of theffect of anethole on transcriptional factors with excessive ROS during adipogenic differentiation. a 2 mM H2O2 was added for 30 min on the first day of adipogenic differentiation. After exposure to H2O2, hMSCs were incubated with adipogenic media for 2 weeks. Whole cell lysates were prepared equally at day 14 as described in Fig. 3. b Each protein expression was exhibited in the same manner as described in Fig. 3b. Each experiment was repeated three times and the values are presented as mean ± S.D. **p < 0.05, and ***p < 0.001