Fig. 6From: Mannan oligosaccharide requires functional ETC and TLR for biological radiation protection to normal cellsDevelopment of ρ° cells from NKE cells. NKE ρ° cells were generated from NKE cells by selectively depleting mitochondrial DNA using ethidium bromide as described in materials and methods. Mitochondrial DNA was isolated from NKE and ρ° cells, and ND1 gene amplicon from mitochondrial DNA (as amplified using PCR) was checked using agarose gel electrophoresisBack to article page