Fig. 4

Clonogenic assay. Effect of pre-irradiation treatment of NKE cells with mannan at different time interval was accessed by using colony forming efficiency (CFE) assay as described in materials and method section. After incubation, formed colonies were fixed, stained and counted. Results are expressed as surviving fraction with respect to control ±SD of three independent experiments. Differences were designated significant at values * p < 0.05, ** p < 0.001 and *** p < 0.0001 and were labeled as # compared with the sham irradiated control group, * compared with the radiation group