Molecular targets and signaling pathways regulated by nuclear translocation of syndecan-1

Table 2 Differentially expressed genes from Affymetrix array, validated by qRT-PCR

Comparison	Gene product	Microarray (FC)	qPCR (FC, relative expression ± SD, P-value)
a. FLs1 vs NLSdel	EGR1 NEK11 DOCK8	1.85 1.77 1.72	1.39 ± 0.06 (p = 0.0004) 1.67 ± 0.41 (p = 0.04) 2.13 ± 0.49 (p = 0.08)
b. FLs1 vs V	COL1A2 PCDH18 ITGA8 PIP5K1B DACH1 AREG SERPINB4	0.58 0.54 0.48 0.47 0.41 0.40 0.36	0.51 ± 0,07 (p = 0.00001) 0.46 ± 0,04 (p = 0.00001) 0.50 ± 0.22 (p = 0.0019) 0.28 ± 0.18 (p = 0.0001) 0.29 ± 0.09 (p = 0.0001) 0.28 ± 0.25 (p = 0.0005) 0.21 ± 0.13 (p = 0.0005)
c. NLSdel vs V	COL19A1 FAP SERPINA3 SERPINB4 IL2RB	0.89 0.59 0.48 0.37 0.33	0.53 ± 0.37 (p = 0.02) 0.67 ± 0.07 (p = 0.00003) 0.59 ± 0.26 (p = 0.009) 0.66 ± 0.24 (p = 0.03) 0.55 ± 0.25 (p = 0.02)

Quantitative real-time PCR analysis of gene expression in B6FS cells overexpressing full-length syndecan-1 (FLs1), cells overexpressing NLS deleted syndecan-1 (NLSdel) and control cells (V). A subset of genes fulfilling the criteria of >1.5 fold up- or downregulation and a false discovery rate (q) ≤0.05 were further analyzed by qPCR. The table shows the significantly altered genes by both microarray and qPCR. FC fold change, SD-standard deviation. qPCR was performed three times, each in triplicates

ISSN: 2661-8850