Fig. 4From: Impact of the MDM2 splice-variants MDM2-A, MDM2-B and MDM2-C on cytotoxic stress response in breast cancer cellsExpression of PUMA upon overexpression of the MDM2 splice variants. Activation of PUMA after transfection with the splice variants by qPCR assay revealing relative mRNA levels of PUMA. MCF-7 cells transfected with pCMV-GFP (GFP-control), MDM2-A, -B and -C, sorted after 12 h based on GFP expression. Graphs show the untreated cells (purple bars) and the 24 h 1 μM doxorubicin (green bars). The experiment was repeated in triplicate, with three independent transfections. * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001Back to article page