Fig. 4

p53 inactivation and lamin A/C suppression lead to malignant tumors. Primary p53 knockout MOSE cells were transfected with control or siRNA (si-Lam A) and cultured for about 2 months. When the lamin A/C-suppressed and p53 (-/-) MOSE cells were implanted in nude mice subcutaneously, invasive tumors developed in 4 weeks (a). Two areas of the tumor are shown in higher magnification (b, c). Three examples of tumors formed from lamin A/C-suppressed p53 (-/-) MOSE cells were stained with lamin A/C, as shown in (d), (e), and (f). g Tumors formed from p53 (-/-) MOSE cells (not treated with lamin A/C-siRNA)) were compared, and a higher magnification (h) is shown. i An example of lamin A/C immunostaining is shown for a tumor derived for p53 (-/-) MOSE cells