Table 1 A list of strains used in this study
Strain Name | Genotype or Description | Ref/Source |
|---|---|---|
BY4741- or 4742-based strains | Mata (BY4741) or Matα (BY4742) his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Open Biosystems |
Opi1-GFP | Opi1-GFP::HIS3 (BY4742), a haploid progeny from crossing BY4741 Opi1-GFP::HIS3 and BY4742 sei1∆::Kan | Open Biosystems |
Scs2-GFP | Scs2-GFP::HIS (BY4742) | Open Biosystems |
sei1Δ | sei1Δ::KanMX (BY4742) | Open Biosystems |
sei1ΔOpi1-GFP | Opi1-GFP sei1Δ::KanMX (BY4742), a haploid progeny from crossing Opi1-GFP::HIS3 (BY4741) and sei1∆::Kan (BY4742) | This study |
sei1ΔScs2-GFP | Scs2-GFP sei1Δ::KanMX (BY4742), a haploid progeny from crossing Scs2-GFP::HIS3 (BY4741) and sei1Δ::Kan (BY4742) | This study |
Opi1FFATmut-GFP | Opi1FFATmut-GFP::KanMX6 (BY4742) | This study |
sei1Δ Opi1FFATmut-GFP | Opi1FFATmut-GFP::KanMX6 (BY4742), a crossed progeny between Opi1FFATmut-GFP and sei1Δ ::Kan (BY4741) | This study |
Pah1-GFP::HIS3 | Pah1-GFP::HIS3 (BY4741) | Open Biosystems |
sei1Δ Pah1-GFP | sei1Δ::Hph Pah1-GFP::HIS3 (BY4741) | This study |
W303-based strains | MATa (W303-1A) or MATα (W303-1B), leu2-3,112 trp1-1 can1-100 ura3- 1 ade2-1 his3-11,15 | Thomas 1989 [35], Open Biosystems |
Opi1-mCherry | Opi1-mCherry::KanMX (W303-1A or 1B) | This study |
sei1Δ | sei1Δ::Hph (W303-1B) | This study |
sei1Δ Opi1-mCherry | sei1Δ::Hph Opi1-mCherry (W303-1B) | This study |
Pah1-3xFlag | Pah1-3xFlag::KanMX (W303-1A) | This study |
sei1Δ Pah-3xFlag | sei1Δ::Hph Pah1-3xFlag::KanMX (W303-1A) | This study |
3KO( Gal DGA1) Opi1-mCherry | 3KO( Gal DGA1) Opi1-mCherry::KanMX | Cartwright et al., 2015 [14]; this study |
3KO( Gal DGA1) sei1Δ Opi1-mCherry | 3KO( Gal DGA1) sei1Δ Opi1-mCherry::KanMX | Cartwright et al., 2015 [14]; this study |
3KO( Gal ARE1) Opi1-mCherry | 3KO( Gal ARE1) Opi1-mCherry::KanMX | Cartwright et al. 2015 [14]; this study |
3KO( Gal ARE1) sei1Δ Opi1-mCherry | 3KO( Gal ARE1) sei1Δ Opi1-mCherry::KanMX | Cartwright et al., 2015 [14]; this study |
gpt2Δ Opi1-mCherry | gpt2Δ::NAT Opi1-mCherry ::KanMX | This study |
gpt2Δ sei1Δ Opi1-mCherry | gpt2Δ::NAT sei1Δ::Hph Opi1-mCherry ::KanMX | This study |
slc1Δ Opi1-mCherry | slc1Δ::NAT Opi1-mCherry ::KanMX | This study |
slc1Δ sei1Δ Opi1-mCherry | slc1Δ::NAT sei1Δ::Hph Opi1-mCherry ::KanMX | This study |
ale1Δ Opi1-mCherry | ale1Δ::NAT Opi1-mCherry ::KanMX | This study |
ale1Δ sei1Δ Opi1-mCherry | ale1Δ::NAT sei1Δ::Hph Opi1-mCherry ::KanMX | This study |
loa1Δ Opi1-mCherry | loa1Δ::NAT Opi1-mCherry ::KanMX | This study |
loa1Δ sei1Δ Opi1-mCherry | loa1Δ::NAT sei1::Hph Opi1-mCherry ::KanMX | This study |
dgk1Δ Opi1-mCherry | dgk1Δ::NAT Opi1-mCherry ::KanMX | This study |
dgk1Δ sei1Δ Opi1-mCherry | dgk1Δ::NAT sei1Δ::Hph opi1::Opi1-mCherry KAN | This study |
spo14Δ Opi1-mCherry | spo14Δ::NAT Opi1-mCherry ::KanMX | This study |
spo14Δ sei1Δ Opi1-mCherry | spo14Δ::NAT sei1Δ::Hph Opi1-mCherry ::KanMX | This study |
ldb16Δ | ldb16Δ::Nat | This study |
sei1Δ ldb16Δ (”ΔΔ”) | sei1Δ::Hph ldb16Δ::Nat | This study |
ldb16∆ Opi1-mCherry | ldb16Δ::Nat Opi1-mCherry::Kan | This study |
sei1∆ldb16∆Opi1-mCherry | sei1Δ::Hph ldb16Δ::Nat Opi1-mCherry::Kan | This study |