Table 1 Plasmids used

pmKate2-lyso

encodes mKate2 N-terminally fused to rat Lamp-1

Evrogen (Russia)

pTagCFP-mito

encodes TagCFP N-terminally fused to mitochondrial targeting sequence derived from the subunit VIII of human cytochrome C oxidase

Evrogen (Russia)

pTagRFP-Golgi

encodes TagRFP N-terminally fused to Golgi targeting sequence of human β-1,4-galactosyltransferase

Evrogen (Russia)

pRab7-EYFP^a

encodes Rab7 C-terminally fused to eYFP

[88]

pRab5-ECFP^a

encodes Rab5 C-terminally fused to eCFP

[88]

pDsRed-Rab5/DN^b

encodes dominant-negative Rab5/N133I N-terminally fused to DsRed

[134,135]/Add #13051^g

pDsRed-Rab7/DN^b

encodes Rab7/T22N N-terminally fused to DsRed

[134,135]/Add #12662^g

pDsRed-Rab9^b

encodes Rab9 N-terminally fused to DsRed

[134,135]/Add #12677^g

pDsRed-Rab11^b

encodes Rab11 N-terminally fused to DsRed

[134,135]/Add #12679^g

pmRFP-LC3^c

encodes autophagosome-specific LC3 protein N-terminally fused to mRFP

[136]/Add #21075^g

pYFP-Rac1(N17)^d

encodes Rac1/T17N N-terminally fused to fluorescent protein YFP

[137]/Add #11395^g

pER-RFP^e

encodes RFP N-terminally fused to CD5 leader sequence and C-terminally fused to SEKDEL amino acid sequence

[43]

pTet-Off Advanced

encodes the tetracycline-controlled transactivator protein tTA-Advanced

Clontech (USA)

pBI-EGFP

allows expression of a gene of interest and marker gene of eGFP under control of the bi-directional tTA-responsive promotor

Clontech (USA)

pHAV3’^f

bears a cDNA encoding a segment of human hepatitis A virus genome (strain HAS-15)

[138]/GenBank: X15463.1

pBI-EGFP/3C

encodes 3Cpro and eGFP under control of bi-directed tTA-responsive promotor.

This study

pBI-EGFP/3CMut

encodes 3Cpro with Cys172→Ala mutation and eGFP under control of bi-directed tTA-responsive promotor.

This study