Figure 5From: Subcellular localization of ammonium transporters in Dictyostelium discoideumAmt Topology. Cytosolic localization of C-terminal YFP tags. The AmtA and AmtB YFP strains (green) were incubated with TRITC-dextran to label endosome lumens (red). After cell lysis, the endosomes were concentrated by centrifugation, and the resuspended pellet was left untreated or treated with proteinase K. Images are confocal with 2 μm scale bars.Back to article page