From: The NIMA-family kinase, Nek1 affects the stability of centrosomes and ciliogenesis
mNek1 aa residues expressed with GFP tag | Subcellular localization of GFP-truncated Nek1 | Percent of transfected cells without γ-tubulin foci |
---|---|---|
1–258 | Cytoplasmic | 0 |
1–352 | Nuclear | 0 |
1–407 | Nuclear | 8 ± 1 |
1–510 | Nuclear | 68 ± 3 |
1–599 | Nuclear | 64 ± 3 |
1–686 | Nuclear | 64 ± 4 |
1–686 (K33R) | Ciliary | 0 |