Figure 5From: The NIMA-family kinase, Nek1 affects the stability of centrosomes and ciliogenesismNek1 disruption of centrosomes is not due to sequential loss. Cells were scored for the presence or absence of cilia and centrosomes following transfection with eGFP-alone (A) and eGFP-mNek1 (1–686) (B). Error bars represent standard deviation of 3 experiments, n = 100 for each experiment. (C and D) Time course of γ-tubulin foci number (0, 1, 2 or > 2) in cells expressing eGFP alone (C) or eGFP-mNek1 (1–686) (D). Cells transfected with eGFP-mNek1 (1–686) are not observed to retain a single γ-tubulin focus, suggesting that loss is coincident.Back to article page