Figure 2From: The NIMA-family kinase, Nek1 affects the stability of centrosomes and ciliogenesisTransiently transfected constructs are first expressed as cells exit mitosis. (A) Representative fluorescence microscopy image of IMCD3 cells expressing eGFP (green) two hours post-transfection connected by a cytokinetic bridge stained with acetylated-tubulin (red). (B) Graph of the presence of cytokinetic structures and primary cilia over a post-transfection time course. Error bars represent the standard deviation of two experiments, n = 100 for each. The high percentage of transfected cells possessing cytokinetic structures two hours post-transfection indicates that these cells recently completed mitosis. Bar, 10 μm.Back to article page