| wt | H40Y | V163L | 164N | N115S | G268R | D268R | G15R |
---|
| und. | diff. | und. | diff. | und. | diff. | und. | diff. | und. | diff. | und. | diff. | und. | diff. | und. | diff. |
n
| 189 | 219 | 174 | 222 | 203 | 189 | 182 | 128 | 52 | 89 | 135 | 127 | 140 | 110 | 114 | 108 |
cat. 1 %
|
74
|
57
|
44
|
41
|
25
|
40
|
15
|
55
|
52
|
67
|
65
|
72
|
14
|
35
|
58
|
42
|
cat. 2 %
|
26
|
3
|
56
|
36
|
75
|
26
|
85
|
5
|
48
|
5
|
35
|
8
|
86
|
44
|
42
|
30
|
aggr. | 32 | 33 | 57 | 19 | 8 | 38 | 11 | 29 | 36 | 75 | 30 | 60 | 17 | 100 | 94 | 19 |
nucl.aggr. | 0 | 0 | 31 | 0 | 56 | 38 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
deloc. | 68 | 11 | 27 | 4 | 42 | 2 | 92 | 43 | 64 | 0 | 70 | 20 | 84 | 0 | 10 | 0 |
aberr. SF | 0 | 67 | 0 | 91 | 0 | 68 | 0 | 57 | 0 | 25 | 0 | 20 | 0 | 12 | 0 | 88 |
cat. 3 %
| |
33
| |
5
| |
7
| |
23
| |
22
| |
17
| |
7
| |
11
|
cat. 4 %
| |
7
| |
18
| |
25
| |
17
| |
2
| |
2
| |
11
| |
18
|
aggr. | | 38 | | 38 | | 35 | | 27 | | 100 | | 100 | | 100 | | 42 |
nucl.aggr. | | 0 | | 21 | | 58 | | 0 | | 0 | | 0 | | 0 | | 0 |
deloc. | | 56 | | 8 | | 2 | | 83 | | 0 | | 0 | | 0 | | 0 |
aberr. SF | | 13 | | 49 | | 40 | | 0 | | 0 | | 0 | | 8 | | 84 |
- Table 1 shows a summary of data from experiments where EGFP-tagged actin constructs were transfected into C2C12 cells and myoblasts were then differentiated for 4–6 days. After immunostaining, transfected myoblasts or differentiated myotubes were classified into categories depending on how well the EGFP-actin was integrated and the differentiation state of the cells:
- myoblasts (und.): category 1: cells with good integration of EGFP-actin construct
- category 2: cells with poor integration of EGFP-actin construct, divided into different subgroups: cytoplasmic aggregates (aggr.), nuclear aggregates (nucl. aggr.), delocalization (deloc.), aberrant stress fibres (aberr. SF)
- myotubes (diff.): category 1: differentiated cells with good integration of EGFP-actin consructs
- category 2: differentiated cells with poor integration (see subgroups above)
- category 3: undifferentiated cells with good integration
- category 4: undifferentiated cells with poor integration (see subgroups above)
- For every construct, the total number of transfected cells that were counted is also stated (n).