Figure 5

Role of CTGF in high glucose-induced migration of cultured HUVSMCs. Quiescent cells were transfected with scrambled or CTGF-siRNA expressing plasmid for 24 hours, then exposed to HG for 48 hours, and followed by the measurement of cell migration in a monolayer scratch wound assay. Figure (a) shows a representative result of three mock transfected experiments (Magnification 200×). Figure (b) shows a representative result of three scrambled siRNA plasmid transfected experiments (Magnification 200×). Figure (c) shows a representative result of three CTGF-siRNA plasmid transfected experiments (Magnification 200×). Figure (d) shows the average of migrated cells in three experiments. * P < 0.05 vs mock transfection or scrambled siRNA transfection.