ANTIBODY | HUMAN BLOOD GRANULOCYTES | MOUSE BLOOD GRANULOCYTES | MOUSE MPRO CELLS** |
---|
dimethyl H3K9 | + N | + N | + N |
trimethyl H3K9 | + N g | + N p | + N p |
monomethyl H3K27 | + N | + N | + N |
dimethyl H3K27 | + N | + N | + N |
trimethyl H3K27 | + N | + N | + N |
dimethyl H4K20 | +/- N, C | +/- N | +/- N |
trimethyl H4K20 | + N, C | + N p | + N p |
HP1 α | 0‡ | 0 | +/- N, C |
HP1 β | 0‡ | 0 | +/- N, C |
HP1 γ | +/- N, C | + N p | + N p |
- *The results listed represent a qualitative assessment, combining data from immunostaining and immunoblotting. In the case of normal mouse blood, due to inadequate enrichment of granulocytes, no immunoblotting was performed.
- ** Identical immunostaining and immunoblotting results were obtained with undifferentiated and RA differentiated MPRO cells.
- Abbreviations: N, nuclear staining; N g, nuclear coarse granules; N p, nuclear pericentric heterochromatin; N, C, nuclear and cytoplasmic staining.
- Reaction intensity: +, moderate to strong; +/-, weak; 0, negligible.
- ‡ Moderate to strong staining in the cytoplasm only.