Figure 1

Determination of the Iporin interacting domain. (A) Schematic overview of the domain structure of rab1b with the conserved G1-G3 boxes, PM1-3 motifs, N-terminal and C-terminal hypervariable regions (hpr) and the C-terminal prenylation site (ps). SF1-4 indicate the rab subfamily regions. The rab1b regions of the chimeras are shaded dark and the rab6 regions are light-shaded. (B) All constructs were co-transformed in yeast and cells were cultivated on selection plates as described above (Table 1). Rab1b deletion mutants were co-transformed with Iporin ΔN847, whereas chimeras were co-transformed with full-length Iporin. - = no growth on selection plates or no β-galactosidase activity; +/- = background growth or β-galactosidase activity appears overnight; ++ = strong growth; +++ = very fast growth or high β-galactosidase activity