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Table 1 Interaction of LPP with Scrib in the yeast two-hybrid system

From: The tumor suppressor Scrib interacts with the zyxin-related protein LPP, which shuttles between cell adhesion sites and the nucleus

BAIT

PREY

HIS

LACZ

pGBT9

pACT2-mScrib

-

-

pGBT9-LPPWT

pACT2

-

-

pGBT9-LPPWT

pACT2-mScrib

+

+

pGBT9-LPPS609A

pACT2

-

-

pGBT9-LPPS609A

pACT2-mScrib

+

+

pGBT9-LPPT610A

pACT2

-

-

pGBT9-LPPT610A

pACT2-mScrib

-

-

pGBT9-LPPD611A

pACT2

-

-

pGBT9-LPPD611A

pACT2-mScrib

+

+

pGBT9-LPPL612A

pACT2

-

-

pGBT9-LPPL612A

pACT2-mScrib

-

-

  1. Yeast cells (CG-1945), cotransformed with a bait and a prey as indicated, were selected on medium containing 5 mM 3-AT, lacking Trp, Leu and His. Yeast colonies were tested for the expression of β-galactosidase.
  2. + indicates strong positive interaction; - indicates no interaction.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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