Figure 8
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
Analysis of the effects of transduction of PBD containing gelsolin peptides and osteopontin on endogenous gelsolin-actin ratio Osteoclasts were transduced with indicated TAT-proteins and treated with OPN (denoted as (+)) or PBS (denoted as (-)). Equal amount of lysates were immunoprecipitated with anti-gelsolin antibody. The EGTA-resistant gelsolin/actin complex was subjected to SDS-PAGE as described in the Methods section. Immunoprecipitation with a non-immune serum (NI) is shown in lane 9. Western analysis was performed with antibodies to gelsolin and actin. Gelsolin, actin, and IgG heavy chain (IgG Hc) bands are indicated by arrows. The quantitative changes in the actin/gelsolin ratio were determined by scanning the gelsolin and actin bands. The mean ± SEM for three experiments is provided in Table 1.