Figure 4
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
The effects of transduction of TAT-fused gelsolin proteins on PtdIns P 2 association with endogenous gelsolin Osteoclasts were transduced with TAT-peptides and treated with PBS (-) or OPN (+) as indicated below the figure. Lysates were immunoprecipitated with a monoclonal antibody to gelsolin (lanes 1–8; 10–17) or non-immune serum (NI; lane 9). Panel A: Western analysis with an antibody to PtdIns P2. Arrows point to endogenous gelsolin (lanes 1–8 and 10–17) and the coprecipitated transduced TAT-gelsolin protein (lanes 16 and 17) associated with PtdIns P2. IgG heavy chain is also indicated by an arrow. Panel B: Western analysis with an antibody to gelsolin. The level of gelsolin in each lane is shown. Arrows indicate endogenous (lanes 1–8 and 10–17) and TAT-gelsolin (lanes 16 and 17) proteins. The results represent one of three experiments performed from three separate osteoclast preparations.