Study methods | Propidium iodide and Annexin V --FITC studies | ||||
---|---|---|---|---|---|
Culture medium | Total n | With PI-positive cells * | With Annexin V-positive and PI-negative cells ** | With Annexin V-positive or PI-positive cells *** | Without Annexin V- and PI-positive cells **** |
kSOM/AA with IGF-1 | 106 | 5(4.7%) All are arrested embryos | 5(8.5%) All are arrested embryos | 10(9.4%)# | 96(86.8%)# All blastocysts and 2 arrested embryos |
kSOM/AA without IGF-1 | 83 | 7(8.4%) All are arrested embryos | 13(15.6%) Arrested embryos and five blastocysts | 20(24.1%)# | 63(75.9%)# All are blastocysts |