Figure 6
From: Characterization of subcellular localization and stability of a splice variant of G alphai2
Myristoylation and palmitoylation of α i2 and sα i2 COS-7 cells were transfected with pcDNA3 (lanes 1 and 4), or pcDNA3 containing αi2 (lanes 2 and 5) or sαi2 (lanes 3 and 6). 48 h after transfection, cells were incubated with 1.0 mCi/ml 3H-palmitate (lanes 1–3) or 0.5 mCi/ml 3H-myristate (lanes 4–6) for 2 h. Following immunoprecitation with the EE antibody, duplicate samples were resolved by SDS-PAGE. Radiolabeled proteins were visualized by fluorography (panel A), and immunoblotting with the EE antibody provides a comparison of protein levels of αi2 and sαi2 (panel B).