Figure 4From: Differential effects of PDCD4 depletion on protein synthesis in myoblast and myotubesInteraction of PDCD4 with eIF4A or eIF4G in myotubes. Myotubes were incubated in differentiation (Ctl) or starvation (serum- and amino acid-free) medium for 12Ā h. They were then harvested (Stv) or refed in the differentiation medium in the presence of rapamycin for 1 or 3Ā h. Lysates were immunoprecipitated with either (A) anti eIF4A or (C) anti PDCD4 antibodies followed by western blotting. Signals from immunoprecipitates were quantified and shown in B, D and E. Means Ā± SE; nā=ā4; bars with different letters differ (Pā<ā0.05).Back to article page