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Table 2 Relative amounts of monomeric and dimeric forms in wild-type TC14s and their mutant proteins.*

From: Tunicate cytostatic factor TC14-3 induces a polycomb group gene and histone modification through Ca2+ binding and protein dimerization

 

Monomer (%)

Dimer (%)

TC14-3, wild

< 1.0

> 99.0

TC14-2, wild

38.9

61.1

TC14-3F65D

100

0

TC14-3T69R

33.3

64.2

TC14-2R69T**

15.7

69.3

  1. *, SDS-PAGE was done under non-heated condition. After staining, each band was scanned with a gel scanner.
  2. **, TC14-2R69T had a few intermediate bands, so that the sum of monomer and dimer did not attain to 100%.