Figure 6

Quantification of in vitro and in vivo PmEed induction by wild-type and mutant TC14-3s. (A, B)Cultured cells. (A)PCR products of PmEed (upper) and Pmβ-actin (lower). Lane 1, control (PBS). Lane 2, wild-type TC14-3. Lane 3, TC14-3^T69R. Lane 4, TC14-3^E106G. (B)PCR products from 25^th to 30^th cycle. Lanes 1-6, control (PBS). Lanes 7-12, wild-type TC14-3. (C-E)Aged zooid pieces. (C)PCR products of PmEed (upper) and β-actin (lower). Lane 1, control (PBS). Lane 2, wild type TC14-3. Lane 3, TC14-3^K113S.N114E. Lane 4, TC14-3^T69R. Lane 5, TC14-3^E106G. (D)PCR products from 25^th to 30^th cycle. Lanes 1-6, control (PBS). Lanes 7-12, wild-type TC14-3. (C)Increasing kinetics of PCR products quantified by ImageJ output.