Figure 4

Effect of genistein on differentiation of LM8 cells. (A) LM8 cells were treated for 3 days without (left panel) or with (right panel) 50 μM genistein, fixed with ethanol, and stained with H&E. Scale bar: 50 μm. (B) LM8 cells were treated for 3 days without or with 50 μM genistein, and the expression of mRNAs of osteocalcin, Drg-1, and β-actin was examined by RT-PCR. (C) LM8 cells were treated for 3 days without (left panel) or with (right panel) 50 μM genistein, and immunohistochemical staining of Drg-1 was performed. Scale bar: 50 μm.