Figure 6From: Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorterSP cells sorted from Msi1high cells could develop into neural and intestinal epithelial tissues. (A): Proliferative curves of SP and NSP cells cultured in vitro. (B): Developmental potentials of SP and NSP cells in vivo. Abundant AD-like, NT-like, and sack-like structures were observed in the grafts from SP cells. Fibrous tissues and nest-like structures were observed in NSP grafts. Bar indicates 25 μm in this panel. (arrowheads; H&E staining) (C): RT-PCR analysis for the markers of neural cells (Nestin and Tubulin β III) and intestinal epithelium cells (SI, Fabp2, Tff3, Lyz1, and ChgA). (D): Relative mRNA expressions of Nestin and Tubulin β III are shown in this panel. (Mean ± SE; n = 3 individual experiments; *P< 0.05 compared with NSP group; N.D indicates not detected) (E): Relative mRNA expressions of SI, Fabp2, Tff3, Lyz1, and ChgA indicated by the integrated intensity in Panels C. (Mean ± SE; n = 3 individual experiments; *p < 0.05 compared with NSP group; N.D indicates not detected) (F): Immunostaining for Tubulin β III and Fabp2 in SP and NSP grafts. Tubulin β III positive cells were located in some of non-specific structures and neural tube-like structures (arrowheads). More Fabp2 positive cells were detected in SP grafts (arrowheads). Partial Fabp2 positive cells formed sack-like structures with a monolayer cell structure (arrow). The special portion was zoomed and magnified. Bar indicates 50 μm in Tubulin β III panels and 100 μm in Fabp2 panels.Back to article page