Figure 5From: The putative Notch ligand HyJagged is a transmembrane protein present in all cell types of adult Hydra and upregulated at the boundary between bud and parentWhole mount in situ hybridisation for Hyjagged and kringelchen during late budding stages. Left hand panel: Budding stages 8, 9 and 10 according to [31]; (A-C) in situ hybridisation for Hyjagged, (D-F') in situ hybridisation for kringelchen from [10]; (F') foot of bud that fell off during staining procedure; (p) parent animal; (b) bud. NBT/BCIP (Roche) was used for staining reactions (blue signals). Scale bars: 20 μm.Back to article page