Figure 1From: Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factorsPromoters activation in EADSCs. A) Transiently transfected EADSCs with constructs containing promoter in front of the luciferase reporter gene or pGL3 empty vector (diagrams not on scale) as a control, at 24 h post transfection. The results are expressed as the mean relative Luciferase units after normalizing with efficiency of transfection. Each reaction was done in quadruplicate, and each point represents the average ± standard deviation from three experiments. B) Efficiency of EADSCs transfection obtained with different transfection methods, using pEGFP-C1 as a reporter plasmid. Each transfection method was done in quadruplicate, and each point represents the average ± standard deviation from three experiments. EGFP was monitored by fluorescence microscope and quantified by flow cytometry.Back to article page