Figure 4
From: Cbl-associated protein is tyrosine phosphorylated by c-Abl and c-Src kinases
Tyrosine mutations do not alter the localization of CAP into focal adhesions and stress fibers. Cells transfected with CAP-EGFP, Y326F, Y360F or Y632F mutant were seeded out on fibronectin coated coverslips, allowed to adhere for 30 min or 120 min, fixed and stained for vinculin (blue) and phalloidin (red). WT CAP, Y326F and Y360F mutant were found to show a similar overall localization, whereas Y632F mutant exhibited some nuclear staining, in addition to focal adhesions and stress fibers. All CAP variants showed two localization patterns, either colocalizing with vinculin in cell matrix adhesions after shorter spreading times (30 min) or with actin fibers after longer times of spreading (120 min).