Figure 2

Subcellular membrane distribution of SLMAP. (A) COS7 cells transiently transfected with 6Myc-tagged SLMAP variants encompassing either TA1 (A; a-c), TA2 (A; d-f) or ΔLZ_TA2 (g-i) were stained with anti-calnexin polyclonal antibodies (b, e, h). The overlay of the anti-Myc and calnexin in these cells is shown in A; c, f, i. SLMAP distribution with respect to the secretory system. (B) COS7 cells transfected with either 6Myc-SLMAP-TA1 (B; a-c) or 6Myc-SLMAP1-TA2 (d-f) were stained with anti-SLMAP (B; a, d) and anti-ERGIC-53 (B; b, e), with the overlay shown in B; c, f. SLMAP distribution with respect to the Golgi apparatus. (C) COS7 cells expressing either 6Myc-SLMAP-TA1 (C; a-c); 6Myc-SLMAP-TA2 (C; d-f) or 6Myc-SLMAPΔLZ-TA2 (g-i), were co-stained with anti-Golgi 58 K (C; b, e, h) and anti-SLMAP (a, d, g), with the overlay shown in C; c, f, i. Scale bar = 10 μm.