Primer | Tm | product | Accession-No. |
---|---|---|---|
5'-CAGTGAATTC ATGGCCGGT TGGCAGAG-3' 5'-CGATGGATCC AGCAGCTAGAACCCAGAGTC-3' | 68°C 71°C | 446 bp ORF (bp 99 → 526) | huProfilin2a NM_053024 |
5'-TTGAATTC ATGAGTGACTGGAAGGGCTACA-3' 5'-TTGGATCC GTTCACGGTTTATTCTGGTCTCC-3' | 65°C 68°C | 467 bp ORF (bp 59 → 524) | mProfilin3 NM_029303 |
5'-CAGTGAATTC AGCATGAGTCACTTGCG-3' 5'-GATGGATCC TTAGTTTCCCTTTTTCCTTAG-3' | 63°C 64°C | 405 bp ORF (bp 861 → 1250) | mProfilin 4 NM_028376 |
5'-AGTGAATTC GGGAACATGAGCCATT-3' 5'-GATGGATCC CTCTGATGACTTAACTTCCT-3' | 61°C 65°C | 406 bp ORF (bp171 → 557) | huProfilin4 BC029523 |
5'-TAGAATTC ATGGATGGATGATATCGCCGCGC-3' 5'-AAGGATCC AAGCCATGCCAATCTCA-3' | 67°C 70°C | 1236 bp ORF (bp74 → 1282) | hu β-actin, NM_001101 |
5'-CAGTGAATTC TTTACCGACCACCAAGAAACTCAG-3' 5'-CGATGGATCC TCATATGGCACCCGAAAAGAGC-3' | 68°C 71°C | 668 bp, FH1 + part. FH2-domain (bp904 → 1551) | huDiaphanous3 NM_030932 |