- Research article
- Open Access
Endoplasmic reticulum degradation impedes olfactory G-protein coupled receptor functional expression
© Lu et al; licensee BioMed Central Ltd. 2004
Received: 02 June 2004
Accepted: 15 September 2004
Published: 15 September 2004
Research on olfactory G-protein coupled receptors (GPCRs) has been severely impeded by poor functional expression in heterologous systems. Previously, we demonstrated that inefficient olfactory receptor (OR) expression at the plasma membrane is attributable, in part, to degradation of endoplasmic reticulum (ER)-retained ORs by the ubiquitin-proteasome system and sequestration of ORs in ER aggregates that are degraded by autophagy. Thus, experiments were performed to test the hypothesis that attenuation of ER degradation improves OR functional expression in heterologous cells.
To develop means to increase the functional expression of ORs, we devised an approach to measure activation of the mOREG OR (Unigene # Mm.196680; Olfr73) through coupling to an olfactory cyclic nucleotide-gated cation channel (CNG). This system, which utilizes signal transduction machinery coupled to OR activation in native olfactory sensory neurons, was used to demonstrate that degradation, both by the ubiquitin-proteasome system and autophagy, limits mOREG functional expression. The stimulatory effects of proteasome and autophagy inhibitors on mOREG function required export from the ER and trafficking through the biosynthetic pathway.
These findings demonstrate that poor functional expression of mOREG in heterologous cells is improved by blocking proteolysis. Inhibition of ER degradation may improve the function of other ORs and assist future efforts to elucidate the molecular basis of odor discrimination.
The sense of smell originates in the olfactory epithelium when olfactory receptors (ORs), members of the seven transmembrane domain G-protein coupled receptor (GPCR) superfamily, bind odorant ligands [1, 2]. Despite identification of the first constituents of the ~1000 member OR superfamily over a decade ago, efforts to uncover the molecular basis of odor discrimination have been severely limited by the inability to efficiently express ORs at the plasma membrane in heterologous expression systems [2–6].
Recently, we elucidated three specific cellular mechanisms responsible for inefficient OR trafficking to the plasma membrane: ORs are retained within the endoplasmic reticulum (ER) due to inefficient folding and poor coupling to ER export machinery, degraded via the ubiquitin-proteasome system, and sequestered in ER aggregates that are degraded by autophagy . Thus, we have a clearer understanding of the problems associated with OR expression in heterologous cells.
To develop rationale means to improve the functional expression of ORs, an approach was devised to quantitate activation of the mouse mOREG OR (Unigene # Mm.196680; Olfr73), which recognizes the odorant eugenol (spicy, cinnamon-like odor) , following coupling to an olfactory cyclic nucleotide-gated cation channel (CNG) . Using this assay, we show that degradation by both the ubiquitin-proteasome system and autophagy limits mOREG functional expression. Our results demonstrate for the first time that inhibition of proteolysis can positively modulate OR function.
Results and discussion
Functional expression of mOREG using a CNG-based assay
A cell-based approach was developed to measure functional expression of mOREG in heterologous cells. This system was designed to mimic the signal transduction events involved in OR activation in the olfactory epithelium and utilizes CNG as a cAMP biosensor [10, 11]. In olfactory sensory neurons, odorant binding to ORs initiates a signaling cascade involving the heterotrimeric G protein Golf, adenylate cyclase III, and an olfactory CNG, leading ultimately to the sensation of smell [1, 12]. Accordingly, we transiently co-expressed mOREG, as an N-terminal fusion protein with the first 20 amino acids of rhodopsin (Rho20-mOREG), with untagged olfactory CNG subunits in HEK293 cells that endogenously express both the heterotrimeric G protein Gs, a functional homologue of Golf [13, 14], and adenylate cyclase III . The Rho tag has been shown to facilitate chemosensory GPCR functional expression [8, 16, 17], possibly by enhancing translocation into the ER during protein synthesis. In this system, odorant binding to Rho-mOREG, which couples to endogenous Gs and elicits increases in the second messenger cAMP in HEK cells [8, 18, 19], leads to the opening of CNG and the influx of calcium from the extracellular medium. Thus, Rho-mOREG function can be directly correlated with cellular calcium levels.
ER degradation limits mOREG functional expression
The non-additive effects of 3-MA and MG-132 on Rho-mOREG function suggest one of the following two non-mutually exclusive scenarios: first, OR degradation by autophagy may be linked to OR degradation by the ubiquitin-proteasome system, by a poorly defined mechanism as suggested for other aggregation prone proteins [23–25]; second, in addition to ER degradation, an additional step(s) downstream of proteolysis may limit Rho-mOREG functional expression in heterologous cells. A recent preliminary report described specialized accessory proteins that increase OR surface expression and function . These proteins could serve as chaperones to package OR cargo into COPII vesicles for export from the ER and/or couple ORs to requisite signal transduction machinery at the plasma membrane, steps that are both downstream of ER degradation. In the absence of necessary accessory proteins, functional expression may not exceed a certain level regardless of the quantity of OR that is diverted from the degradative pathways by autophagy and ubiquitin-proteasome inhibitors. The existence of multiple steps limiting OR functional expression in heterologous cells is further supported by our findings that the function of mOREG, lacking the Rho tag, is less robust than Rho-mOREG. Since the Rho tag may facilitate translocation into the ER during protein synthesis [8, 16, 17], ER translocation could comprise an additional limiting step, upstream of ER degradation, for OR functional expression.
ER export and trafficking through the Golgi apparatus are necessary for Rho-mOREG functional expression
Inhibition of ER degradation events could permit a pool of Rho-mOREG to achieve an ER export competent conformation and traffic through the Golgi apparatus to the plasma membrane. However, using surface biotinylation, surface immunofluorescence microscopy, flow cytometry, and glycosidase digestion assays, we were unable to demonstrate convincing Rho-mOREG surface expression or visualize a pool of Rho-mOREG containing endoglycosidase H-resistant carbohydrate modifications indicative of transit through the Golgi apparatus following treatment with ER degradation inhibitors (ML and BDM unpublished observations). These results suggested that either a small pool of properly folded Rho-mOREG was expressed at the plasma membrane in quantities below the threshold of the cell biological techniques employed to visualize the receptor, or that a pool of intracellular Rho-mOREG comprised the functionally responsive population in calcium imaging experiments. Notably, numerous studies have documented the functional expression of GPCRs and requisite signal transduction machinery in intracellular compartments, including ER membranes [27–29].
To differentiate between these two possibilities, we adopted a pharmacological approach to selectively and independently block trafficking from compartments in the early secretory pathway, specifically the ER and Golgi apparatus. If inhibition of ER degradation does not increase Rho-mOREG activity under conditions that block export from ER and Golgi compartments, the functionally responsive Rho-mOREG population is likely derived from an internal pool that is required to traffic to the plasma membrane to function. Conversely, if inhibition of ER degradation increases Rho-mOREG activity under conditions that block export from ER and Golgi compartments, the functionally responsive Rho-mOREG population likely resides in an intracellular compartment.
To inhibit protein trafficking from the Golgi apparatus, monensin, an ionophore that disrupts Golgi structure and inhibits Golgi trafficking events, was used . Monensin, similar to BFA, completely blocked the enhancement of Rho-mOREG function by 3-MA and MG-132 while having no effect on β-AR function (Fig. 5A). Similar effects were observed when cells were incubated at 20°C (Fig. 5A), a temperature that arrests protein transport at trans Golgi cisternae . Importantly, trafficking disrupting agents did not affect Rho-mOREG or β-AR function when acutely applied to cells, further substantiating that results were not attributable to non-specific effects on signal transduction machinery or eugenol binding (Fig. 5B). Thus, Golgi trafficking events are required for increased Rho-mOREG functional expression by degradation inhibitors.
Inhibition of Rho-mOREG function by trafficking disrupting agents, specifically BFA, could be due to activation of the unfolded protein response  and inhibition of Rho-mOREG protein synthesis. To directly address this possibility, experiments were performed to test the effect of the protein synthesis inhibitor cycloheximide (CHX) on functional expression of Rho-mOREG. As shown in Figure 5C, CHX, used at concentrations previously demonstrated to inhibit Rho-mOREG translation , had no effect on increased Rho-mOREG function by 3-MA and MG-132. These data suggest that autophagy and ubiquitin-proteasome inhibitors diverted an existing pool of Rho-mOREG from degradative pathways to the secretory pathway and that effects of degradation inhibitors and trafficking disrupting agents were not attributable to modulation of protein synthesis.
Collectively, these data support a model whereby inhibition of ER degradation promotes a small fraction of Rho-mOREG to achieve an ER export competent conformation, thereby satisfying ER quality control processes, and traffic through the secretory pathway to the plasma membrane. Thus, similar to chemical and pharmacological chaperones that promote folding and restore ER export of misfolded GPCR cargo [35–37], agents interfering with ER degradation may promote ER export of GPCR and non-GPCR cargo , trafficking through the biosynthetic pathway, and functional expression at the cell surface. We speculate that the pool of Rho-mOREG expressed at the plasma membrane is below the detection limits of cell biological techniques used to visualize the receptor  but above the detection threshold for calcium imaging methodology used to examine receptor function. Indeed, following pharmacological treatments, improved ΔF508 cystic fibrosis transmembrane conductance regulator functional expression at the plasma membrane is readily detectable by sensitive electrophysiological analyses but neither by cell surface labeling nor by biochemical approaches measuring carbohydrate modifications indicative of transit through the Golgi apparatus [39–41]. Though we favor a model whereby inhibition of ER degradation promotes OR export from the ER and improves OR surface expression, we were unable to obtain cellular and biochemical evidence to support this proposal. We cannot exclude the possibility that inhibition of ER degradation also stabilizes an otherwise labile cofactor or chaperone protein, endogenously expressed by heterologous cells, that modulates OR trafficking in a post-Golgi compartment, stability at the cell surface, and/or function at the plasma membrane [42, 43].
We have developed an expression system for ORs that utilizes signal transduction machinery coupled to OR activation in native olfactory sensory neurons. Using CNG as a cAMP biosensor to gauge mOREG function, we demonstrate that inhibition of ER degradation, by both autophagy and the ubiquitin-proteasome system, promotes functional expression of Rho-mOREG as well as untagged mOREG. Thus, proteolysis limits mOREG function in heterologous cells. Inhibition of ER degradation may improve the function of other ORs and assist future efforts to elucidate the molecular basis of odor discrimination.
Rho20-mOREG expression vector was generated in pRK5 as previously described . To generate untagged mOREG expression vector, mOREG coding sequence was excised using AscI/NotI and subcloned into a modified pRK5 vector lacking the Rho20 tag. The human CNGA2 and CNGB1b expression constructs encode untagged human CNGA2 and CNGB1b in pEAK10-derived vectors (Edge Biosystems, Gaithersburg, MD) . For the generation of stable transfectants, CNGA2 was subcloned into pCDNA3.1/zeo (Invitrogen, Carlsbad, CA). The CNGA2 clone contains the C458W and E581M mutations, introduced using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA), previously shown to increase cAMP sensitivity in rat CNGA2 .
Compounds, odorants, and ligands
BFA, isoproterenol, monensin, and 3-MA were from Sigma (St. Louis, MO); MG-132 was from Calbiochem (San Diego, CA); eugenol, heptanal and octanal were from Aldrich (Milwaukee, WI).
Cell culture and transfections
HEK293 cells were maintained and transfected as previously described . For the generation of CNG stable transfectants, cells were transfected with linearized CNGA2 and CNGB1b expression constructs (1:1 ratio) and selected using 50 ug/mL zeocin (Invitrogen) and 0.5 ug/mL puromycin (Calbiochem). Individual colonies were expanded and screened for CNG expression by assaying functional responses to 500 uM eugenol following transient transfection with Rho-mOREG. For functional expression studies, CNG cells were grown in media without selection 72 h prior to experimentation and transiently transfected with mOREG 48 h prior to experimentation.
mOREG functional expression
Functional expression of mOREG was investigated using calcium imaging methodology as previously described . Cells seeded in 24 well plates were loaded with the calcium dye fluo-4 acetoxymethyl ester (Molecular Probes, Eugene, OR) 2 d post-transfection using the following conditions: 3 uM dye in 0.5 ml Hanks' balanced salt solution containing divalent cations (HBSS, Invitrogen) for 1 h at room temperature in the dark. Cells were subsequently washed once with 0.5 ml HBSS to remove excess fluo-4, supplemented with 0.25 ml HBSS, and then stimulated with an additional 0.25 ml HBSS containing the appropriate ligand at twice the final concentration. A single ligand was applied to each dish of cells. Typically, 3–4 separate dishes of cells were used for each ligand concentration or condition and experiments were repeated 3–4 times. Thus, individual data points represent the average of 9–16 separate measurements.
Changes in intracellular calcium were monitored by fluorescence microscopy using an Axiovert S100 TV inverted microscope with a 10× long working distance Plan Fluor objective (numerical aperture 0.5) and a cooled charge-coupled device camera (Princeton Instruments, Trenton, NJ). Images were acquired using a Lambda DG-4 automated wavelength controller (Sutter Instrument Co., Novato, CA) at 480 nm excitation and 535 nm emission and analyzed using Imaging Workbench 4.0 (Axon Instruments, Union City, CA). Counting the number of cells responding to ligands 60 sec following stimulus addition, when cells had achieved a maximal response, quantitated receptor activity. This established methodology has been used to functionally characterize the human T1R1/T1R3 umami receptor, the human T1R2/T1R3 sweet receptor, and the Drosophila Gr5a trehalose receptor [44, 45]. To independently validate this method, we determined that the EC50 for isoproterenol activation of the β-AR (3.7 +/- 1.0 nM), measured by counting responding cells, closely matched published values (1.7–3.3 nM), measured either by fluorescent intensity measurements or a cAMP accumulation assay . In addition, the EC50 for eugenol activation of Rho-mOREG (20.8 +/- 3.4 uM), measured by counting responding cells, closely approximated the published value (35 uM and 46 uM), determined by monitoring fluorescent intensity of responding cells [8, 19]. Finally, the EC50s for glutamate activation of mGluR4 and cycloheximide activation of mT2R05 were similar when determined by counting cells or by monitoring fluorescent intensity .
We estimate ~40–50% of transfected CNG cells express functional cell surface Rho-mOREG based on the following points. First, ~20–25% of total cells in a microscopic field respond to maximal doses of eugenol. Second, ~50% of cells are transfected, measured by either co-transfection with red fluorescent protein or by immunolabelling permeabilized cells expressing Rho-mOREG with an anti-Rho antibody. Thus, ~40–50% of transfected cells express sufficient Rho-mOREG at the plasma membrane to elicit a functional response.
Data represent the mean +/- SEM. Statistical significance was determined using an unpaired, two-tailed Student's t-test. Dose-response curves were plotted and EC50 values were determined using GraphPad Prism v3.02 software.
We thank Elliot Adler for important contributions with CNG cloning as well as Xiaodong Li, Alexey Pronin, Guy Servant, Mark Zoller, and Lubert Stryer for critical review of the manuscript.
- Firestein S: How the olfactory system makes sense of scents. Nature. 2001, 413: 211-218. 10.1038/35093026.View ArticlePubMedGoogle Scholar
- Mombaerts P: How smell develops. Nat Neurosci. 2001, 4 Suppl: 1192-1198. 10.1038/nn751.View ArticlePubMedGoogle Scholar
- Buck L, Axel R: A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell. 1991, 65: 175-187. 10.1016/0092-8674(91)90418-X.View ArticlePubMedGoogle Scholar
- Mombaerts P: Genes and ligands for odorant, vomeronasal and taste receptors. Nat Rev Neurosci. 2004, 5: 263-278. 10.1038/nrn1365.View ArticlePubMedGoogle Scholar
- Gimelbrant AA, Stoss TD, Landers TM, McClintock TS: Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells. J Neurochem. 1999, 72: 2301-2311. 10.1046/j.1471-4159.1999.0722301.x.View ArticlePubMedGoogle Scholar
- McClintock TS, Sammeta N: Trafficking prerogatives of olfactory receptors. Neuroreport. 2003, 14: 1547-1552. 10.1097/00001756-200308260-00001.View ArticlePubMedGoogle Scholar
- Lu M, Echeverri F, Moyer BD: Endoplasmic reticulum retention, degradation, and aggregation of olfactory g-protein coupled receptors. Traffic. 2003, 4: 416-433. 10.1034/j.1600-0854.2003.00097.x.View ArticlePubMedGoogle Scholar
- Kajiya K, Inaki K, Tanaka M, Haga T, Kataoka H, Touhara K: Molecular bases of odor discrimination: Reconstitution of olfactory receptors that recognize overlapping sets of odorants. J Neurosci. 2001, 21: 6018-6025.PubMedGoogle Scholar
- Kaupp UB, Seifert R: Cyclic nucleotide-gated ion channels. Physiol Rev. 2002, 82: 769-824.View ArticlePubMedGoogle Scholar
- Reinscheid RK, Kim J, Zeng J, Civelli O: High-throughput real-time monitoring of G(s)-coupled receptor activation in intact cells using cyclic nucleotide-gated channels. Eur J Pharmacol. 2003, 478: 27-34. 10.1016/j.ejphar.2003.08.036.View ArticlePubMedGoogle Scholar
- Rich TC, Tse TE, Rohan JG, Schaack J, Karpen JW: In vivo assessment of local phosphodiesterase activity using tailored cyclic nucleotide-gated channels as cAMP sensors. J Gen Physiol. 2001, 118: 63-78. 10.1085/jgp.118.1.63.PubMed CentralView ArticlePubMedGoogle Scholar
- Ronnett GV, Moon C: G proteins and olfactory signal transduction. Annu Rev Physiol. 2002, 64: 189-222. 10.1146/annurev.physiol.64.082701.102219.View ArticlePubMedGoogle Scholar
- Jones DT, Reed RR: Golf: an olfactory neuron specific-G protein involved in odorant signal transduction. Science. 1989, 244: 790-795.View ArticlePubMedGoogle Scholar
- Jones DT, Masters SB, Bourne HR, Reed RR: Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf. J Biol Chem. 1990, 265: 2671-2676.PubMedGoogle Scholar
- Xia Z, Choi EJ, Wang F, Storm DR: The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons. Neurosci Lett. 1992, 144: 169-173. 10.1016/0304-3940(92)90742-P.View ArticlePubMedGoogle Scholar
- Krautwurst D, Yau KW, Reed RR: Identification of ligands for olfactory receptors by functional expression of a receptor library. Cell. 1998, 95: 917-926. 10.1016/S0092-8674(00)81716-X.View ArticlePubMedGoogle Scholar
- Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng L, Guo W, Zuker CS, Ryba NJ: T2Rs function as bitter taste receptors. Cell. 2000, 100: 703-711. 10.1016/S0092-8674(00)80706-0.View ArticlePubMedGoogle Scholar
- Katada S, Nakagawa T, Kataoka H, Touhara K: Odorant response assays for a heterologously expressed olfactory receptor. Biochem Biophys Res Commun. 2003, 305: 964-969. 10.1016/S0006-291X(03)00863-5.View ArticlePubMedGoogle Scholar
- Katada S, Tanaka M, Touhara K: Structural determinants for membrane trafficking and G protein selectivity of a mouse olfactory receptor. J Neurochem. 2004, 90: 1453-1463. 10.1111/j.1471-4159.2004.02619.x.View ArticlePubMedGoogle Scholar
- Zhao H, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K, Firestein S: Functional expression of a mammalian odorant receptor. Science. 1998, 279: 237-242. 10.1126/science.279.5348.237.View ArticlePubMedGoogle Scholar
- Seglen PO, Gordon PB: 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc Natl Acad Sci U S A. 1982, 79: 1889-1892.PubMed CentralView ArticlePubMedGoogle Scholar
- Meng L, Mohan R, Kwok BH, Elofsson M, Sin N, Crews CM: Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity. Proc Natl Acad Sci U S A. 1999, 96: 10403-10408. 10.1073/pnas.96.18.10403.PubMed CentralView ArticlePubMedGoogle Scholar
- Kopito RR, Sitia R: Aggresomes and Russell bodies. Symptoms of cellular indigestion?. EMBO Rep. 2000, 1: 225-231. 10.1093/embo-reports/kvd052.PubMed CentralView ArticlePubMedGoogle Scholar
- Fortun J, Dunn W. A., Jr., Joy S, Li J, Notterpek L: Emerging role for autophagy in the removal of aggresomes in Schwann cells. J Neurosci. 2003, 23: 10672-10680.PubMedGoogle Scholar
- Ravikumar B, Duden R, Rubinsztein DC: Aggregate-prone proteins with polyglutamine and polyalanine expansions are degraded by autophagy. Hum Mol Genet. 2002, 11: 1107-1117. 10.1093/hmg/11.9.1107.View ArticlePubMedGoogle Scholar
- Saito H, Matsunami M, Roberts R, Chi Q, Matsunami H: Molecules that regulate translocation and function of mammalian odorant receptors [abstract]. AChems. 2004, 26: 33 (abstract # 128)-Google Scholar
- Gobeil F., Jr., Vazquez-Tello A, Marrache AM, Bhattacharya M, Checchin D, Bkaily G, Lachapelle P, Ribeiro-Da-Silva A, Chemtob S: Nuclear prostaglandin signaling system: biogenesis and actions via heptahelical receptors. Can J Physiol Pharmacol. 2003, 81: 196-204. 10.1139/y02-163.View ArticlePubMedGoogle Scholar
- Boivin B, Chevalier D, Villeneuve LR, Rousseau E, Allen BG: Functional endothelin receptors are present on nuclei in cardiac ventricular myocytes. J Biol Chem. 2003, 278: 29153-29163. 10.1074/jbc.M301738200.View ArticlePubMedGoogle Scholar
- O'Malley KL, Jong YJ, Gonchar Y, Burkhalter A, Romano C: Activation of metabotropic glutamate receptor mGlu5 on nuclear membranes mediates intranuclear Ca2+ changes in heterologous cell types and neurons. J Biol Chem. 2003, 278: 28210-28219. 10.1074/jbc.M300792200.View ArticlePubMedGoogle Scholar
- Klausner RD, Donaldson JG, Lippincott-Schwartz J: Brefeldin A: insights into the control of membrane traffic and organelle structure. J Cell Biol. 1992, 116: 1071-1080. 10.1083/jcb.116.5.1071.View ArticlePubMedGoogle Scholar
- Jockers R, Angers S, Da Silva A, Benaroch P, Strosberg AD, Bouvier M, Marullo S: Beta(2)-adrenergic receptor down-regulation. Evidence for a pathway that does not require endocytosis. J Biol Chem. 1999, 274: 28900-28908. 10.1074/jbc.274.41.28900.View ArticlePubMedGoogle Scholar
- Mollenhauer HH, Morre DJ, Rowe LD: Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity. Biochim Biophys Acta. 1990, 1031: 225-246. 10.1016/0304-4157(90)90008-Z.View ArticlePubMedGoogle Scholar
- Kuismanen E, Saraste J: Low temperature-induced transport blocks as tools to manipulate membrane traffic. Methods Cell Biol. 1989, 32: 257-274.View ArticlePubMedGoogle Scholar
- Zhang K, Kaufman RJ: Signaling the unfolded protein response from the endoplasmic reticulum. J Biol Chem. 2004, 279: 25935-25938. 10.1074/jbc.R400008200.View ArticlePubMedGoogle Scholar
- Morello JP, Petaja-Repo UE, Bichet DG, Bouvier M: Pharmacological chaperones: a new twist on receptor folding. Trends Pharmacol Sci. 2000, 21: 466-469. 10.1016/S0165-6147(00)01575-3.View ArticlePubMedGoogle Scholar
- Petaja-Repo UE, Hogue M, Bhalla S, Laperriere A, Morello JP, Bouvier M: Ligands act as pharmacological chaperones and increase the efficiency of delta opioid receptor maturation. Embo J. 2002, 21: 1628-1637. 10.1093/emboj/21.7.1628.PubMed CentralView ArticlePubMedGoogle Scholar
- Li T, Sandberg MA, Pawlyk BS, Rosner B, Hayes KC, Dryja TP, Berson EL: Effect of vitamin A supplementation on rhodopsin mutants threonine-17 --> methionine and proline-347 --> serine in transgenic mice and in cell cultures. Proc Natl Acad Sci U S A. 1998, 95: 11933-11938. 10.1073/pnas.95.20.11933.PubMed CentralView ArticlePubMedGoogle Scholar
- Novoradovskaya N, Lee J, Yu ZX, Ferrans VJ, Brantly M: Inhibition of intracellular degradation increases secretion of a mutant form of alpha1-antitrypsin associated with profound deficiency. J Clin Invest. 1998, 101: 2693-2701.PubMed CentralView ArticlePubMedGoogle Scholar
- Lim M, McKenzie K, Floyd AD, Kwon E, Zeitlin PL: Modulation of deltaF508 cystic fibrosis transmembrane regulator trafficking and function with 4-phenylbutyrate and flavonoids. Am J Respir Cell Mol Biol. 2004, 31: 351-357. 10.1165/rcmb.2002-0086OC.View ArticlePubMedGoogle Scholar
- deCarvalho AV, Ndi CP, Tsopmo A, Tane P, Ayafor J, Connolly JD, Teem JL: A novel natural product compound enhances cAMP-regulated chloride conductance of cells expressing CFTR[delta]F508. Mol Med. 2002, 8: 75-87.PubMed CentralPubMedGoogle Scholar
- Loffing-Cueni D, Loffing J, Shaw C, Taplin AM, Govindan M, Stanton CR, Stanton BA: Trafficking of GFP-tagged DeltaF508-CFTR to the plasma membrane in a polarized epithelial cell line. Am J Physiol Cell Physiol. 2001, 281: C1889-97.PubMedGoogle Scholar
- Wojcikiewicz RJ: Regulated ubiquitination of proteins in GPCR-initiated signaling pathways. Trends Pharmacol Sci. 2004, 25: 35-41. 10.1016/j.tips.2003.11.008.View ArticlePubMedGoogle Scholar
- Shenoy SK, McDonald PH, Kohout TA, Lefkowitz RJ: Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin. Science. 2001, 294: 1307-1313. 10.1126/science.1063866.View ArticlePubMedGoogle Scholar
- Li X, Staszewski L, Xu H, Durick K, Zoller M, Adler E: Human receptors for sweet and umami taste. Proc Natl Acad Sci U S A. 2002, 99: 4692-4696. 10.1073/pnas.072090199.PubMed CentralView ArticlePubMedGoogle Scholar
- Chyb S, Dahanukar A, Wickens A, Carlson JR: Drosophila Gr5a encodes a taste receptor tuned to trehalose. Proc Natl Acad Sci U S A. 2003, 100 Suppl 2: 14526-14530. 10.1073/pnas.2135339100.View ArticlePubMedGoogle Scholar
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