- Research article
- Open Access
Differential localization in cells of myosin II heavy chain kinases during cytokinesis and polarized migration
© Liang et al; licensee BioMed Central Ltd. 2002
Received: 2 April 2002
Accepted: 24 July 2002
Published: 24 July 2002
Cortical myosin-II filaments in Dictyostelium discoideum display enrichment in the posterior of the cell during cell migration and in the cleavage furrow during cytokinesis. Filament assembly in turn is regulated by phosphorylation in the tail region of the myosin heavy chain (MHC). Early studies have revealed one enzyme, MHCK-A, which participates in filament assembly control, and two other structurally related enzymes, MHCK-B and -C. In this report we evaluate the biochemical properties of MHCK-C, and using fluorescence microscopy in living cells we examine the localization of GFP-labeled MHCK-A, -B, and -C in relation to GFP-myosin-II localization.
Biochemical analysis indicates that MHCK-C can phosphorylate MHC with concomitant disassembly of myosin II filaments. In living cells, GFP-MHCK-A displayed frequent enrichment in the anterior of polarized migrating cells, and in the polar region but not the furrow during cytokinesis. GFP-MHCK-B generally displayed a homogeneous distribution. In migrating cells GFP-MHCK-C displayed posterior enrichment similar to that of myosin II, but did not localize with myosin II to the furrow during the early stage of cytokinesis. At the late stage of cytokinesis, GFP-MHCK-C became strongly enriched in the cleavage furrow, remaining there through completion of division.
MHCK-A, -B, and -C display distinct cellular localization patterns suggesting different cellular functions and regulation for each MHCK isoform. The strong localization of MHCK-C to the cleavage furrow in the late stages of cell division may reflect a mechanism by which the cell regulates the progressive removal of myosin II as furrowing progresses.
Most animal cells are constantly rearranging their cellular structures to optimally perform their functions or to respond appropriately to the changing environment that surrounds them. Using a simple protein "building block" that has the ability to self-associate to form massive structural arrays is a common theme used in creating a dynamic cytoskeleton. Temporal and spatial regulation of this self-assembly and its associated disassembly process is critical for correct function. For a model system, we have focused on the dynamics of myosin II thick filaments in D. discoideum. This protein forms a self-assembled, highly regulated bi-directional array of molecules that together with actin filaments are capable of producing force for cellular rearrangements. All evidence suggests that unless this molecule is assembled into its appropriate thick filament array it cannot function to produce force.
Eukaryotic cells during cell division construct contractile rings that are mainly composed of an actin-based cytoskeleton. Myosin II, a key component of this actin-based cytoskeleton, has been shown to be essential for cytokinesis of D. discoideum cells in suspension as well as for efficient chemotaxis and morphogenetic changes in shape during development) [1–3]. All of these roles require myosin II to be in the form of thick filaments. The question of how myosin II thick filament assembly is regulated within living cells, however, remains mostly unanswered. The amoeba D. discoideum has a number of advantages as a model system to study in vivo regulation of myosin II thick filament assembly. D. discoideum has only one endogenous copy of the myosin II heavy chain gene, and null strains of myosin II are available) [1, 2]). Cytokinesis in D. discoideum is also morphologically very similar to that of animal cells).
In D. discoideum, myosin II molecules are constantly relocating into multiple locations for participating in various processes. Dynamic exchange occurs between a cytosolic soluble pool and assembled filaments that are enriched in the cortical cytoskeleton. The half-life of myosin between these pools has been measured to be ~ 7 sec, indicating the importance of dynamic assembly control in the localization of the protein). When a cell migrates, myosin II accumulates in the posterior of the cell. During cell division, myosin II accumulates in the cleavage furrow in the early stages of cytokinesis. To accomplish its cellular tasks, myosin II assembles into bipolar thick filaments and pull together oppositely oriented actin filaments to produce contractile forces. Mutant forms of myosin II that do not assemble into bipolar thick filaments in vitro fail to rescue myosin null phenotypes, nor do they localize to the furrow during cytokinesis [6, 7]). Although myosin II is not essential for cell division on a surface, it is critical for normal timely cell separation and for symmetric placement of the division furrow . GFP-myosin II is transported to the furrow of dividing cells growing on surfaces even though it is not essential for cytokinesis under these conditions.
The assembly of myosin II monomers into filaments is regulated by phosphorylation of its heavy chains at three threonine residues at the C-terminus of the tail [9, 10]. Dephosphorylation of these threonines is a prerequisite of filament assembly, as confirmed by the phenotypes of a 3xAsp mutant, in which the three threonines are replaced by three aspartate residues (mimicking the phosphorylated state) . In vitro the 3xAsp myosin II is severely impaired for filament assembly, and in vivo 3xAsp myosin II fails to assemble or localize to the cortical cytoskeleton. Cells expressing this myosin thus recapitulate the defects of myosin II null cells, including failure to develop normally and failure to divide in suspension. In contrast, cells expressing a non-phosphorylatable myosin II construct (3xAla myosin cells) display severe myosin overassembly into the cytoskeleton , and excessive myosin localization to the cleavage furrow during cytokinesis . The 3xAla myosin cells also display severe defects in chemotactic cell migration, demonstrating the importance of proper myosin II assembly dynamics in this process .
Myosin II heavy chain kinase (MHCK) activity in this system capable of disassembling myosin II filaments in vitro was originally reported with partially enriched kinase fractions . The enzyme MHCK-A was subsequently purified to homogeneity and shown to be capable of driving myosin II filament disassembly in vitro via myosin II heavy chain phosphorylation [14, 15]). A MHCK-A cDNA was cloned via expression cloning and peptide sequence derived from the native enzyme . This enzyme is now recognized as the founding member of a highly novel family of protein kinases unrelated to conventional protein kinases, with members present in D. discoideum and throughout the animal kingdom. Homology-based cloning and genomic approaches led to the identification of two closely related D. discoideum enzymes, MHCK-B  and MHCK-C (GenBank accession AAC31918, and ). Several enzymes present in mammalian systems are now recognized as having the same conserved catalytic domain, including the eEF-2 kinases  and TRP-PLIK, a bifunctional protein with kinase and ion channel activities . Biochemical analysis with MHCK-B has confirmed that this enzyme phosphorylates D. discoideum myosin II in vitro [17, 22]). Analysis reported here confirms that MHCK-C also readily phosphorylates D. discoideum myosin II in vitro, supporting a possible direct role for this enzyme in myosin II localization control in vivo.
Although substantial biochemical analysis has been performed with this family of D. discoideum MHCKs, there is relatively little information on the cellular distribution of the endogenous MHCKs in vivo. Previously, Steimle and colleagues  reported that in interphase cells, during chemotaxis, random migration, and endocytic/phagocytic events, MHCK-A is recruited preferentially to anterior F-actin rich protrusions. With the discovery of two additional myosin II heavy chain kinases, MHCK-B and MHCK-C, we report here in live migrating and cytokinetic cells a systematic in vivo examination of these three MHCKs labeled with GFP.
This analysis reveals striking differences in localization between these three enzymes. During cytokinesis, MHCK-A displays weak enrichment at the cell poles, while MHCK-B displays a mostly diffuse localization. In contrast, MHCK-C displays strong localization to the cleavage furrow only during the late stages of cell division. These results suggest that D. discoideum cells use a family of related MHCKs to modulate myosin II filament assembly, each with distinct roles.
MHCK domain organization and MHCK C biochemical activity
MHCKs have different subcellular localizations in interphase cells
To gain insights into the relative cellular roles and localization of MHCK-A, MHCK-B, and MHCK-C, we have evaluated the behavior of GFP fusions corresponding to each of these enzymes. Distribution of GFP-labelled MHCKs (GFP-MHCK-A, -B and -C) was examined in live AX2 cells (containing an endogenous mhcA gene). These GFP-MHCK expressing cells were able to sporulate and grow in suspension, indicating that at the expression level of these clonal cell lines, the expression of GFP-MHCKs in the AX2 cells does not detectably change myosin II expression or function. The fluorescence distributions of these cells were compared with cells expressing GFP-myosin II, obtained by transforming myosin null cells with a plasmid that carries GFP-mhcA-containing plasmid p102 (Materials and Methods) designated as GFP-myosin II cells hereafter.
In free-moving cells, GFP-MHCK-A was frequently transiently enriched in the protruding edge (Fig. 5-A, bottom), and hence results in rather noticeable pseudopods at the anterior region compared with that in the GFP-myosin II cells. A time-lapse movie in Quicktime format illustrating this behavior is available as an additional file (see additional file 1). GFP-MHCK-B, however, displayed no indication of transient enrichment in any part of the cells while moving; instead it distributes homogeneously within cells (Fig. 5-B, bottom). The cells expressing GFP-MHCK-B appeared to have smooth cell edges because the fluorescence did not label the dynamic pseudopods at the leading edge of the cell, compared with that in GFP-MHCK-A cells. In contrast to MHCK-A and MHCK-B distribution, GFP-MHCK-C was frequently enriched in the posterior cortex of the moving cells (Fig. 5-C, bottom), as seen also for GFP-myosin II (Fig. 5-D, bottom). GFP-MHCK-C occasionally displayed transient enrichment in pseudopodial extensions as well (data not shown).
Dynamic localization of GFP-myosin II and GFP-MHCK-C in the cortex of living D. discoideum cells
As shown above, in interphase GFP-myosin II and GFP-MHCK-C expressed in the presence of myosin II both concentrate in the cell cortex. The actin-rich cortex is estimated to be approximately 0.1–0.2 μm thick in D. discoideum cells , similar to the thickness in other eukaryotic cells . This dimension makes total internal reflection fluorescence (TIRF) microscopy an attractive tool to examine cortical GFP-labelled proteins at the cell-surface contacts. Total internal reflection occurs when light travelling in a medium with high refractive index encounters a medium with low refractive index beyond the critical angle, determined by the ratio of the two refractive indices according to the Snell's law . In our experiments, the coverslip and the cells represent the media with high and low refractive indices, respectively. Under this condition, there is still an exponentially-decayed, evanescent wave penetrating into the D. discoideum cells. The typical depth of the evanescent wave is in the range of 100–200 nm away from the coverslip, which is suitable for exciting cortical GFP-proteins in living D. discoideum cells.
GFP-MHCK-C expressing cells (in the presence of myosin II), display punctate aggregates of fluorescence, with the longer dimension approximately 0.30 μm (standard deviation = 0.05, n = 165, Fig. 6B). The shorter dimension is about 0.15–0.2 μm, similar to what was observed in myosin II thick filaments. The GFP-MHCK-C fluorescent aggregates showed dynamic motility similar to that of GFP-myosin II aggregates. These structures could not be detected when GFP-MHCK-C was expressed in myosin II null cells (data not shown), suggesting that the formation of the GFP-MHCK-C punctate aggregates is myosin II-dependent.
Differential localization of GFP-MHCKs during cytokinesis
Neither GFP-MHCK-A (Fig. 7-A) or -B (Fig. 7-B) was observed to be concentrated in the furrow region during any stage of cytokinesis; nor did they localize to the posterior region of the two daughter cells at the late stage of cytokinesis. Instead, GFP-MHCK-A was enriched in the protrusions extending from the poles of the dividing cells, which resulted in a more prominent appearance of the ruffling polar pseudopods throughout the cytokinesis process. GFP-MHCK-B, however, stayed homogeneously cytoplasmic during cytokinesis without any sign of enrichment in any region. It was excluded from the polar protrusions, as seen by the smooth contour of the poles (Fig. 7-B). Interestingly, as the cells progressed from the early to late stages of cytokinesis, GFP-MHCK-B appeared to be excluded from the furrow (Fig 7-B), as compared to the appearance of GFP-MHCK-A or GFP-MHCK-C.
The localization pattern of GFP-MHCK-C was different from the other two kinases (Fig. 7-C). At the early stage of cytokinesis, GFP-MHCK-C generally displayed a cytosolic distribution with some uniform cortical enrichment, but showed no sign of furrow concentration. However, at the late stage of cytokinesis, as soon as the two daughter cells begin to separate from each other, GFP-MHCK-C became detectable at the newly formed posterior regions of the two daughter cells. The spatial localization of GFP-MHCK-C is similar to that observed for GFP-myosin II at the late stage of cytokinesis (Fig. 7-B and 7-M). In summary, these three GFP-MHCKs have distributions that are temporally and spatially different, as summarized in the sketches shown in Figure 7 bottom.
Localization of GFP-MHCKs in the absence of myosin II
The results reported here provide biochemical and cellular evidence indicating that D. discoideum contains a related family of MHC kinase isoforms that display distinct modes of regulation in vitro and distinct localization dynamics in vivo during contractile events, particularly during cytokinesis. Although MHCK-A has been extensively characterized at the biochemical level [18, 22, 25, 31], only limited biochemical analysis has been performed with bacterially-expressed subdomains of MHCK-B and MHCK-C [17, 18, 22]. The current biochemical results provide strong support for the hypothesis that MHCK-C acts as a MHC kinase in vivo. Further studies with second messenger compounds may help to identify upstream physiological mechanisms that regulate MHCK-C autophosphorylation/activation.
Using epi-fluorescence microscopy, we observe strikingly different patterns of dynamic localization for MHCK-A, -B, and -C during polarized migration and cytokinesis. The dynamics of MHCK-C localization are particularly intriguing, with global or posterior cortical enrichment observed during interphase, with a dramatic accumulation in the furrow during late cytokinesis. The apparent absence of MHCK-C from the furrow in early/mid cytokinesis, when myosin II is clearly accumulating, suggests that specific regulatory mechanisms may exist to recruit this enzyme to the furrow during late cytokinesis.
Co-localization of a MHCK with its apparent substrate does not imply that the kinase, in vivo, is actively phosphorylating its substrate. The dynamic localization of a kinase is only one way to regulate its activity. In fact, the MHCKs are very likely to be highly regulated enzymes; previous studies have documented the in vitro regulation of MHCK A by autophosphorylation, myosin filaments, and acidic phospholipids , and data presented here documents that MHCK C can also be regulated via autophosphorylation. Further studies are needed to confirm similar regulation in vivo, and to better define the upstream regulatory pathways. With these caveats, the distinct localization patterns for MHCK-A, -B, and -C reported here provide valuable clues as to which spatial and temporal myosin II population may be acted upon by each enzyme.
The dynamics of the three MHCKs also display striking differences in their dependence on myosin II. When the GFP fusions are imaged in myosin II null cells, both MHCK-A and MHCK-B display dynamics indistinguishable from their behaviour in cells wild type for myosin II. In contrast, loss of cortical and furrow localization is seen for GFP-MHCK-C in the absence of myosin II. This result suggests that MHCK-C localization in these settings may be achieved via direct association with myosin II filaments. Our TIRF studies further support this model, suggesting that MHCK-C may physically associate with myosin II filaments. GFP-MHCK-C under the TIRF microscope displayed short particles with a longer dimension approximately half of the length of myosin II thick filaments. The bare zone of purified wild-type myosin II thick filaments was estimated previously to be in the range of 0.13–0.19 μm . Based upon these results, we suggest that GFP-MHCK-C may colocalize with myosin II thick filaments by binding at the bare zone.
We suggest that MHCK-C is recruited to the contractile ring during late cytokinesis to facilitate the orderly removal of excess myosin II from the ring as the furrow ingresses. It is particularly intriguing that MHCK-C colocalizes with myosin II in the furrow only at the culmination of cytokinesis where turnover and mobilization of thick filaments might be most appropriate. At this time the cell cycle contraction force requirements are predicted to fall  and the cell's geometrical changes would require myosin II thick filaments to disassemble. Although it is clear throughout the animal kingdom and in protozoa that the mass of myosin II in the division furrow decreases steadily with furrow ingression, no mechanism has been identified in any other system that can explain this regulated disassembly. Dynamic changes in MHC phosphorylation levels within the contractile ring have been reported in dividing sea urchin embryos , suggesting that MHC phosphorylation as a mechanism regulating furrow myosin II disassembly may occur in other systems besides D. discoideum. We suggest that MHCK-C participates in this regulated myosin II filament disassembly in D. discoideum, and that this function may be regulated at both the cellular and biochemical level.
We suggest that differential localization of MHCKs occurs in D. discoideum cells for the purpose of regulating myosin II filament assembly levels in the context of specific cellular contractile events such as lamellipodium extension and cytokinetic furrowing. The late appearance of MHCK-C during furrowing suggests a cellular mechanism regulating its localization, and our biochemical data suggest that MHCK-C phosphorylation levels may represent a mechanism for the fine-tuning of the activity of MHCK-C in the cleavage furrow during cell division. This level of regulation could be mediated via second messenger control of autophosphorylation, or via direct MHCK-C phosphorylation by other kinases. Further studies are in progress to test these models.
Materials and Methods
The GFP fusions to MHCK A, MHCK B, and MHCK C were constructed by placing GFP at the amino-terminus of each kinase coding region, with GFP fused to codon 2 of each kinase open reading frame. All fusions were made in the GFP expression vector pTX-GFP . The construct for MHCK A has been described previously . Protein sequences for MHCK A, MHCK B, and MHCK C correspond to GenBank entries A55532, AAB50136, and AAC31918, respectively. Cloning of the cDNA encoding MHCK-C has been described .
FLAG-MHCK-C purification and phosphorylation assays
A FLAG epitope was fused to the amino-terminus of MHCK-C at codon 2 using the vector pTX-FLAG . The resulting plasmid, pTX-MKC2, was transformed into the cell line Ax2 and clonal cell lines were selected with G418 (8 μg/ml) in HL-5 medium. To facilitate FLAG-MHCK-C protein purification, Ax2/pTX-MKC2 cell lines were then subjected to incremental increases in G418 selection level over approximately 3 weeks, to a final selection level of 40 μg/ml. As reported previously for expression of MHCK-A , this selection process resulted in cell lines with increased expression level of FLAG-MHCK-C, several-fold higher than the initial expression level. In previous work, when this method was applied to MHCK-A-expressing cell lines the elevated expression of MHCK-A resulted in myosin II hyperphosphorylation and myosin II filament disassembly, and corresponding loss of ability of cells to grow in suspension . We observed the same effect in the current studies in attempting to force high expression of FLAG-MHCK-C. The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
For purification of FLAG-MHCK-C, 8–10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 106 cells/ml. All subsequent steps were performed at 0–4°. Cells were harvested by centrifugation (40–50 g typical yield), then washed once in 50 mM Tris, 150 mM NaCl, pH 7.5 (TBS). Cells were resuspended with 4 ml/g cells in 50 mM Tris pH 8, 1 mM DTT, 1 mM EDTA. Protease inhibitor cocktails PIC1 and PIC2  from a 1000X stock were then added to 5X final concentration, and cells lysed either by sonication or by repeated douncing. Lysate was adjusted to 300 mM NaCl (to dissociate MHCK-C from binding to particulate material), then subjected to centrifugation at 125,000 × g for 20 min. The resulting cleared supernatant was brought to 30% saturation with powdered ammonium sulfate and incubated with stirring for 30 min. The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2. This sample was mixed with 2 ml of anti-FLAG epitope monoclonal antibody-conjugated-beads (M2 beads, Sigma) for 2 hours at 4°. Resin was then washed 3X by centrifugation with 10 ml TBSE and transferred to a small disposable column. Resin was washed with a low concentration of competing FLAG peptide (Sigma; 10 μg/ml in TBSE), then the column was eluted with 2 ml of competing FLAG peptide at 120 μg/ml in TBSE. Eluted protein was concentrated by dialysis against powdered polyethylene glycol (Aquacide; Calbiochem), then dialyzed into 20% glycerol, 20 mM Tris pH 7.5, 20 mM KCl, 1 mM EDTA, 1 mM DTT. This purified FLAG-MHCK C was then aliquoted and stored at -80°C.
Dictyostelium myosin II was purified as described . Phosphorylation reactions with myosin II and FLAG-MHCK-C were performed in 20 mM TES pH 7, 2 mM MgCl2, and 0.5 mM ATP, with 32P-γ-ATP included at 800 Ci/mol final concentration. Peptide phosphorylation assays were done with the peptide MH-1 as substrate at 50 μM. This 16-residue peptide corresponds to the phosphorylation target site at position 2029 of MHC. This peptide and the filter-binding assay used to measure its phosphorylation have been described previously . All peptide phosphorylation studies were performed under conditions in which no more than 30% of the substrate was consumed to ensure linear reaction rates.
The catalytic domain of MHCK-C was expressed in bacteria and purified using nickel chelation chromatography as described . Purified protein was injected into New Zealand White rabbits at two week intervals for a total of five injections, followed by a terminal bleed. Western blots were performed using total serum at a dilution of 1/2000 and chemiluminscent detection.
pTX-MKA1, pMKB-GFP, pTX-MKC1 were transformed into either Ax2 cell line  or HS1 myosin null cells, a strain of D. discoideum with its endogenous mhc A gene deleted  to generate GFP-MHCK-A, -B, and -C cells, respectively. p102  which encodes GFP-myosin II, was transformed into the HS1 myosin null cells.
Transformations were performed by electroporation  and transformed cells were selected by 7.5 μg/ml G418 (Geneticin; Life Technologies, Gaithersburg, MD). D. discoideum cells expressing GFP-MHCK's as well as GFP-myosin II-expressing cells were grown and maintained in DdHL-5 media  supplemented with 60 μg/ml streptomycin, 60 U/ml penicillin and 7.5 μg/ml G418. Bacterial lawns were prepared by spreading 2 ml of an overnight Klebsiella aerogenes culture on SM/5 plates .
Cells were imaged in a chambered coverslip (Nunc, Naperville, IL) filled with MES buffer (20 mM 2-[morpholino]ethane-sulfonic acid, pH 6.8, 0.2 mM CaCl2, 2 mM MgSO4) at 21°C. Imaging was done as previously described (Sabry et al., 1997) with the following modifications. The light source was replaced to a Xe arc lamp that has 60% increase over the typical input power of an epi-fluorescent lamp and therefore delivers higher output. This is beneficial when viewing low GFP-expressing cells. For imaging cells reported here, the light was typically attenuated to between 3% to 10% output. The output of the lamp is directed to the microscope by a liquid light-guide. This set up greatly reduces the effect of vibration and heat on the samples and the microscope. Images were collected and analyzed using MetaMorph software (Universal Imaging Corp., West Chester, PA).
Total internal reflection fluorescence microscopy
Using an objective-typed TIRF microscopy, cells were imaged in an anodized aluminum imaging chamber filled with MES buffer (described above) through a coverslip with a refractive index of 1.78. GFP-protein molecules were excited by 488 nm photons produced from an Argon ion laser (Melles Griot) and a 100× (NA = 1.65) objectiv (Olympus). Fluorescent emission was detected through a PentaMax intensified CCD (Princeton Intruments). Typically, each movie collected by Winview (Roper Scientific) was 1 frame/sec for 200 sec, and was subsequently analyzed using NIH image. The apparent length of fluorescent objects was determined using NIH Image (National Institute of Health) to enlarge and identify large (> 10 fold) differences in pixel intensity to identity object edges using the "line-scan" function. Objects that were not clearly separate were removed from consideration."
We thank Ron Rock and Doug Robinson for the help of TIR fluorescent microscopy. This work was supported by National Institutes of Health grant 5R01 GM46551-10 to J. A. Spudich and National Institutes of Health grant R01 GM50009 to T.T. Egelhoff.
- De Lozanne A, Spudich JA: Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science. 1987, 236: 1086-1091.View ArticlePubMedGoogle Scholar
- Knecht DA, Loomis WF: Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science. 1987, 236: 1081-1086.View ArticlePubMedGoogle Scholar
- Wessels D, Soll DR, Knecht D, Loomis WF, De Lozanne A, Spudich J: Cell motility and chemotaxis in Dictyostelium amebae lacking myosin heavy chain. Dev Biol. 1988, 128: 164-177.View ArticlePubMedGoogle Scholar
- Glotzer M: Animal cell cytokinesis. Annu Rev Cell Dev Biol. 2001, 17: 351-386. 10.1146/annurev.cellbio.17.1.351.View ArticlePubMedGoogle Scholar
- Yumura S: Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells. J Cell Biol. 2001, 154: 137-146. 10.1083/jcb.200011013.PubMed CentralView ArticlePubMedGoogle Scholar
- Fukui Y, De Lozanne A, Spudich JA: Structure and function of the cytoskeleton of a Dictyostelium myosin-defective mutant. J Cell Biol. 1990, 110: 367-378.View ArticlePubMedGoogle Scholar
- Sabry JH, Moores SL, Ryan S, Zang JH, Spudich JA: Myosin Heavy Chain Phosphorylation Sites Regulate Myosin Localization during Cytokinesis in Live Cells. Mol Biol Cell. 1997, 8: 2605-2615.PubMed CentralView ArticlePubMedGoogle Scholar
- Weber I, Neujahr R, Du A, Kohler J, Faix J, Gerisch G: Two-step positioning of a cleavage furrow by cortexillin and myosin II. Curr Biol. 2000, 10: 501-506. 10.1016/S0960-9822(00)00452-8.View ArticlePubMedGoogle Scholar
- Luck-Vielmetter D, Schleicher M, Grabatin B, Wippler J, Gerisch G: Replacement of threonine residues by serine and alanine in a phosphorylatable heavy chain fragment of Dictyostelium myosin II. FEBS Lett. 1990, 269: 239-243. 10.1016/0014-5793(90)81163-I.View ArticlePubMedGoogle Scholar
- Vaillancourt JP, Lyons C, Côté GP: Identification of two phosphorylated threonines in the tail region of Dictyostelium myosin II. J Biol Chem. 1988, 263: 10082-10087.PubMedGoogle Scholar
- Egelhoff TT, Lee RJ, Spudich JA: Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo. Cell. 1993, 75: 363-371.View ArticlePubMedGoogle Scholar
- Stites J, Wessels D, Uhl A, Egelhoff T, Shutt D, Soll DR: Phosphorylation of the Dictyostelium myosin II heavy chain is necessary for maintaining cellular polarity and suppressing turning during chemotaxis. Cell Motil Cytoskeleton. 1998, 39: 31-51. 10.1002/(SICI)1097-0169(1998)39:1<31::AID-CM4>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Kuczmarski ER, Spudich JA: Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments. Proc Natl Acad Sci U S A. 1980, 77: 7292-7296.PubMed CentralView ArticlePubMedGoogle Scholar
- Côté GP, Bukiejko U: Purification and characterization of a myosin heavy chain kinase from Dictyostelium discoideum. J Biol Chem. 1987, 262: 1065-1072.PubMedGoogle Scholar
- Côté GP, McCrea SM: Selective removal of the carboxyl-terminal tail end of the Dictyostelium myosin II heavy chain by chymotrypsin. J Biol Chem. 1987, 262: 13033-13038.PubMedGoogle Scholar
- Futey LM, Medley QG, Côté GP, Egelhoff TT: Structural analysis of myosin heavy chain kinase A from Dictyostelium. Evidence for a highly divergent protein kinase domain, an amino-terminal coiled-coil domain, and a domain homologous to the β-subunit of heterotrimeric G proteins. J Biol Chem. 1995, 270: 523-529. 10.1074/jbc.270.2.523.View ArticlePubMedGoogle Scholar
- Clancy CE, Mendoza MG, Naismith TV, Kolman MF, Egelhoff TT: Identification of a Protein Kinase from Dictyostelium with Homology to the Novel Catalytic Domain of Myosin Heavy Chain Kinase A. J Biol Chem. 1997, 272: 11812-11815. 10.1074/jbc.272.18.11812.View ArticlePubMedGoogle Scholar
- Luo X, Crawley SW, Steimle PA, Egelhoff TT, Côté GP: Specific phosphorylation of threonine by the dictyostelium myosin ii heavy chain kinase family. J Biol Chem. 2001, 276: 17836-17843. 10.1074/jbc.M009366200.View ArticlePubMedGoogle Scholar
- Ryazanov AG, Pavur KS, Dorovkov MV: Alpha-kinases: a new class of protein kinases with a novel catalytic domain [letter]. Curr Biol. 1999, 9: R43-R45. 10.1016/S0960-9822(99)80005-0.View ArticlePubMedGoogle Scholar
- Ryazanov AG, Ward MD, Mendola CE, Pavur KS, Dorovkov MV, Wiedmann M: Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc Natl Acad Sci U S A. 1997, 94: 4884-4889. 10.1073/pnas.94.10.4884.PubMed CentralView ArticlePubMedGoogle Scholar
- Runnels LW, Yue L, Clapham DE: TRP-PLIK, a bifunctional protein with kinase and ion channel activities. Science. 2001, 291: 1043-1047. 10.1126/science.1058519.View ArticlePubMedGoogle Scholar
- Steimle PA, Naismith T, Licate L, Egelhoff TT: WD Repeat Domains Target Dictyostelium Myosin Heavy Chain Kinases by Binding Directly to Myosin Filaments. J Biol Chem. 2001, 276: 6853-6860. 10.1074/jbc.M008992200.View ArticlePubMedGoogle Scholar
- Steimle PA, Yumura S, Côté GP, Medley QG, Polyakov MV, Leppert B: Recruitment of a myosin heavy chain kinase to actin-rich protrusions in Dictyostelium. Curr Biol. 2001, 11: 708-713. 10.1016/S0960-9822(01)00182-8.View ArticlePubMedGoogle Scholar
- Kolman MF, Egelhoff TT: Dictyostelium myosin heavy chain kinase A subdomains. Coiled-coil and WD repeat roles in oligomerization and substrate targeting. J Biol Chem. 1997, 272: 16904-16910. 10.1074/jbc.272.27.16904.View ArticlePubMedGoogle Scholar
- Medley QG, Gariepy J, Côté GP: Dictyostelium myosin II heavy-chain kinase A is activated by autophosphorylation: studies with Dictyostelium myosin II and synthetic peptides. Biochemistry. 1990, 29: 8992-8997.View ArticlePubMedGoogle Scholar
- Hanakam F, Albrecht R, Eckerskorn C, Matzner M, Gerisch G: Myristoylated and non-myristoylated forms of the pH sensor protein hisactophilin II: intracellular shuttling to plasma membrane and nucleus monitored in real time by a fusion with green fluorescent protein. EMBO J. 1996, 15: 2935-2943.PubMed CentralPubMedGoogle Scholar
- Shroeder TE: The contractile ring and furrowing in dividing cells. Ann NY Acad Sci. 1990, 582: 78-87.View ArticleGoogle Scholar
- Axelrod D, Hellen EH, Fulbright RM: Total internal reflection fluorescence. In Topics in Fluorescence Spectroscopy: Biochemical Applications. Edited by: Lakowicz J. 1992, New York: Plenum Press, 289-Google Scholar
- Clarke M, Spudich JA: Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. J Mol Biol. 1974, 86: 209-222.View ArticlePubMedGoogle Scholar
- Yumura S, Fukui Y: Reversible cyclic AMP-dependent change in distribution of myosin thick filaments in Dictyostelium. Nature. 1985, 314: 194-196.View ArticlePubMedGoogle Scholar
- Medley QG, Lee SF, Côté GP: Purification and characterization of myosin II heavy chain kinase A from Dictyostelium. Methods Enzymol. 1991, 196: 23-34.View ArticlePubMedGoogle Scholar
- Medley QG, Bagshaw WL, Truong T, Côté GP: Dictyostelium myosin II heavy-chain kinase A is activated by heparin, DNA and acidic phospholipids and inhibited by polylysine, polyarginine and histones. Biochim Biophys Acta. 1992, 1175: 7-12. 10.1016/0167-4889(92)90003-T.View ArticlePubMedGoogle Scholar
- Bray D, White JG: Cortical flow in animal cells. Science. 1988, 239: 883-888.View ArticlePubMedGoogle Scholar
- Robinson DN, Cavet G, Warrick HM, Spudich JA: Quantitation of the Distribution and Flux of Myosin-II during Cytokinesis. BMC Cell Biology. 2002, 3: 4-10.1186/1471-2121-3-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Larochelle DA, Epel D: Myosin Heavy Chain Dephosphorylation During Cytokinesis in Dividing Sea Urchin Embryos. Cell Motil. 1993, 25: 369-380.View ArticleGoogle Scholar
- Levi S, Polyakov M, Egelhoff TT: Green Fluorescent Protein and Epitope Tag Fusion Vectors for Dictyostelium discoideum. Plasmid. 2000, 44: 231-238. 10.1006/plas.2000.1487.View ArticlePubMedGoogle Scholar
- Watts DJ, Ashworth JM: Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970, 119: 171-174.PubMed CentralView ArticlePubMedGoogle Scholar
- Ruppel KM, Uyeda TQ, Spudich JA: Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin. J Biol Chem. 1994, 269: 18773-18780.PubMedGoogle Scholar
- Egelhoff TT, Titus MA, Manstein DJ, Ruppel KM, Spudich JA: Molecular genetic tools for study of the cytoskeleton in Dictyostelium. Methods Enzymol. 1991, 196: 319-334.View ArticlePubMedGoogle Scholar
- Manstein DJ, Schuster HP, Morandini P, Hunt DM: Cloning vectors for the production of proteins in Dictyostelium discoideum. Gene. 1995, 162: 129-134. 10.1016/0378-1119(95)00351-6.View ArticlePubMedGoogle Scholar
- Sussman M: Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. Methods Cell Biol. 1987, 28: 9-29.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.