PKC-δ mediates interferon-α-induced apoptosis through c-Jun NH2-terminal kinase activation
© Yanase et al; licensee BioMed Central Ltd. 2012
Received: 6 May 2011
Accepted: 21 March 2012
Published: 21 March 2012
Interferon-α (IFN-α) exerts an anti-tumor effect at least through induction of apoptosis in a variety of types including B lymphoma cells. We recently found that IFN-α induced a sustained activation of c-Jun NH2-terminal kinase1 (JNK1), which is implicated in activation of the tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) promoter. In the present study, we explored upstream component(s) of the prolonged IFN-α-initiated activation of JNK1.
IFN-α caused activation of PKC-δ in Daudi B lymphoma cells and myeloma U266 cells, as detected by Western blotting using a monoclonal antibody specific for the phosphorylated form of PKC-δ. The dominant-negative form of mutant PKC-δ (dnPKC-δ) reduced the IFN-α-induced JNK1 activation, TRAIL promoter activity, loss of mitochondrial membrane potential (ΔΨm), and increase in propidium iodide (PI) positive cells. The IFN-α-induced activation of JNK1 and the TRAIL promoter was also attenuated by the PKC-δ inhibitor rottlerin. Moreover, a constitutively active form of mutant PKC-δ enhanced the IFN-α-induced TRAIL promoter activity and loss of ΔΨm in Daudi B lymphoma cells. In addition, IFN-α-induced Ser727 phosphorylation of Stat1 was also abrogated by dnPKC-δ.
IFN-α induced JNK1 activation via PKC-δ, leading to upregulation of TRAIL. The interaction of the consequent enhanced TRAIL expression with TRAIL-receptor results in a loss of ΔΨm and increase in PI positive cells. The IFN-α-induced apoptotic events may also be affected by the Ser727-Stat1 induced by PKC-δ-mediated signaling component(s).
Type I interferon-α (IFN-α) has been employed for treatment of patients with some tumors including hairy cell leukemia, chronic myelogenous leukemia, melanoma, and renal cell cancer [1, 2]. The anti-tumor action of IFN-α is mediated at least by induction of apoptosis or inhibition of cell growth [3–6]. However, the detailed molecular mechanism(s) by which IFN-α induces apoptosis remain largely unclear. The interaction of IFN-α with its receptor results in transphosphorylation of receptor-associated Janus kinases (Jak1 and Tyk2), leading to tyrosine phosphorylation of critical residues on the receptors [7, 8]. The activated Jak1/Tyk2 phosphorylate and activate signal transducers and activators of transcription (STAT), which in turn translocates to the nucleus to regulate gene transcription .
In addition to Jak/Stat pathways, mitogen-activated protein kinases (MAPKs) including extracellular kinase 2 (ERK2), p38MAPKs, and c-Jun NH2-terminal kinases (JNKs) are activated in response to IFN-α in some cell types including B lymphoma cells [5, 9–12]. For instance, IFN-α induced prolonged JNK1 activation [5, 12] with decreased ERK activity, resulting in dysfunction of mitochondrial membrane potential (ΔΨm). p38MAPK was also activated by IFN-α [13, 14]. Recent studies have determined that MAPKs activated by IFN-α receptors (IFNARs) are downstream of several components including protein kinase C (PKC) [10, 15]. The PKC family proteins are divided into three subclasses [16, 17]: 1) atypical PKCs (ζ and λ), which are activated by phosphatidylserine; 2) conventional PKCs (α, β, and γ), which are calcium-dependent and activated by diacylglycerol; and 3) the novel PKCs (δ, ε, and θ), which are calcium-independent and activated by diacylglycerol. A novel ubiquitously expressed PKC, PKC-δ, is phosphorylated and activated in response to multiple agents including IFN-α [18–21]. The activated PKC-δ mediates apoptosis in some cell types and survival in other cell types [15, 22, 23].
In the present study, we examined whether PKC-δ is involved in the IFN-α-induced apoptosis in Daudi B lymphoma and U266 myeloma cells. Our data clearly indicate that IFN-α-induced PKC-δ signaling leads to activation of JNK1 and Stat1. These observations would be useful for the development of new treatment modalities of patients by using IFN-α
IFN-α induces phosphorylation of PKC-δ in both daudi B lymphoma and U266 myeloma cells
PKC family proteins have multiple effects on both pro-apoptotic and anti-apoptotic functions [15, 22, 23]. The levels of PKC family proteins in Daudi B lymphoma cells were determined by Western blotting. Substantial levels of PKC-α, -δ, and -ι were detected in Daudi cells, with minute amounts of PKC-γ and -η (Hayashida et al., unpublished observations). PKC-ε and -λ were almost undetectable. Because PKC-δ exerts a pro-apoptotic effect in some cell types , we examined whether PKC-δ is involved in IFN-α-mediated apoptosis in human B lymphoma cells.
PKC-δ inhibitor rottlerin prevents IFN-α-induced PKC-δ activation
PKC-delta inhibitor rottlerin reduces IFN-α-induced activation of TRAIL promoter
IFN-α-induced mitochondrial membrane potential and apoptosis are reduced in Daudi cells overexpressing dominant-negative PKC-δ, while it is partially enhanced in those overexpressing constitutively active PKC-δ
The cell lines overexpressing dnPKC-δ (#1 and #2) showed a reduced loss of ΔΨm and percentage of PI positive cells after IFN-α stimulation (Additional file 2 and Figure 4B and 4C), while the cells expressing caPKC-δ showed an enhanced loss of ΔΨm (Figure 4B). Even in the unstimulated condition, caPKC-δ-overexpressing cells exhibited an increased loss of ΔΨm compared with control vector alone, suggesting that PKC-δ functions as a pro-apoptotic molecule in the IFN-α-induced apoptosis in Daudi B lymphoma cells.
IFN-α-induced activation of JNK and TRAIL promoter is partially reduced in daudi cells overexpressing dnPKC-δ
IFN-α-induced phosphorylation ser-727 of Stat1 is substantially reduced in dnPKC-δ-expressing cells
IFN-α has been employed for treatment of tumors and viral diseases (hepatitis C) [1, 2]. Type I IFN-α exerts multiple functions including anti-viral action, immune modulation, cell proliferation, and cell death [3, 4, 26–28]. IFN-α actions against tumors involve induction of apoptosis as well as indirect effects through induction of cytotoxic cells [7, 29]. We have previously demonstrated that IFN-α induced a sustained activation of pro-apoptotic molecule JNKs , concomitant with upregulation of anti-apoptotic molecule c-Flip through NF-κB activation . However, the molecular mechanisms of IFN-α-induced apoptotic cell death are not completely understood. In the present study, we explored the upstream component of IFN-α-induced JNK activation and found that PKC-δ activation occurs upstream of JNK and Stat1 activation.
Several studies have demonstrated that PKC-δ has multiple targets in response to apoptotic stimuli, including IFN-α [15, 22, 23]. For example, it has been shown that PKC-δ mediates JNK activation in response to DNA damage . Consistent with this observation, genetic silencing of PKC-δ using dnPKC-δ reduced the IFN-α-induced JNK activation (Figure 5A), supporting the notion that PKC-δ signaling participates in the IFN-α-induced JNK activation. Because PKC-δ did not translate to the nucleus in response to IFN-α (Additional file 4), it is unlikely that PKC-δ affects gene expression via transcription factor. Rather, downstream component of PKC-δ signaling pathway including JNK may participate in the upregulation of TRAIL promoter activity .
The interaction of TRAIL with its receptors TRAIL-Rs leads to caspase-8 activation through recruitment of adaptor protein Fas-associated death domain (FADD) . The caspase-8 activation in most cell types is amplified in the mitochondria. The IFN-α-induced loss of ΔΨm and increase in sub-G1 fraction was reduced in the dnPKC-δ-overexpressing cells, while the mitochondrial dissipation was enhanced in the caPKC-δ-overexpressing cells. PKC-δ inhibitor rottlerin was not suitable for analysis of IFN-α-induced loss of ΔΨm in Daudi cells, because rottlerin alone induced mitochondrial dissipation (Additional file 1, [24, 32]). Such rottlerin-induced mitochondrial damage may lead to a small level of PKC-δ activation. Thus, the following pathway is proposed for the IFN-α-induced apoptosis: IFN-α induces JNK1 activation via PKC-δ → upregulation of TRAIL → interaction of TRAIL with its TRAIL-Rs. → loss of ΔΨm → apoptosis. Interestingly, a recent report clearly demonstrated that TRAIL induces PKC-δ activation through diacylglycerol in Jurkat cells , which can form a positive feedback loop involving PKC-δ and TRAIL. Alternatively, it is also possible that the activated JNK induces migration of p21Bax-α to the mitochondria from cytosol, which is cleaved into p18Bax-α, a more potent form [34, 35]. These changes lead to loss of ΔΨm, especially in the later phases of apoptotic processes, which is TRAIL-independent .
The TRAIL promoter contains a possible IFN-stimulated response element (ISRE) and AP-1 sites [37–39], which might be involved in TRAIL promoter activation. Originally, JNK was found to induce phosphorylation of c-Jun, which combines with c-Fos, creating a heterodimer of c-Jun/c-Fos (AP-1) . Indeed, IFN-α caused AP-1 activation in several cell lines including Daudi lymphoma cells, probably through JNK activation (Yanase et al. ms. in preparation, ). The ISRE binds transcription factor IFN-stimulated gene factor-3 (ISGF3) comprising Stat1, Stat2, and IRF-9 . IFN-α caused phosphorylation of Stat1 on Ser727, at least through PKC-δ signaling [19, 21]. We also confirmed the phosphorylation of Stat1 (Ser727) in the IFN-α-stimulated Daudi cells (Figure 6A). The IFN-α-stimulated phosphorylation of Stat1 on Ser727, but not Tyr701, was markedly abrogated in the dnPKC-δ-expressing Daudi cells, confirming that PKC-δ signaling plays a crucial role in the Ser727-PKC-δ phosphorylation. Stat1 activation favours induction of apoptosis in some settings, whereas Ser727-Stat1 promotes survival in others probably through gene induction [40–42]. Experiments are required to address whether IFN-α-induced Ser727-Stat1 functions as proapoptotic or anti-apoptotic molecule.
Several kinases phosphorylate and activate PKC-δ following stimulation with IFN-α. For example, tyrosine phosphorylation of PKC-δ on Tyr 311 was induced by Src-tyrosine kinase [43, 44]. Interestingly, PI3-K and phosphoinositide-dependent kinase (PDK)1  phosphorylated PKC-δ on the Thr505 residue that is stimulated by IFN-α (Figure 1). It remains unclear what kinases are involved in IFN-α-stimulated phosphorylation of PKC-δ in Daudi B lymphoma cells.
Stimulation of IFN-α together with chemotherapeutic agents appears to be effective for treatment of cancer patients [46, 47]. PKC-δ-JNK signaling pathway is involved in induction of apoptosis in response to chemotherapeutic agents . Here, we demonstrated that the IFN-α-induced apoptosis also involves PKC-δ-JNK signaling pathway in human Daudi B lymphoma cells. Concurrent with the activation of pro-apoptotic pathway, we recently demonstrated that IFN-α-induced-PKC-α activation caused upregulation of anti-apoptotic c-Flip through NF-κB activation . Thus, the balance between IFN-α-induced pro-apoptotic (such as PKC-δ-JNK) and anti-apoptotic (such as PKC-α-NF-κB) signaling pathways might determine the cell phenotype. These findings would be valuable for the design of treatment modalities of cancer patients with IFN-α together with or without chemotherapeutic agents.
IFN-α is known to induce JNK activation leading to upregulation of TRAIL promoter activity. In the present study, we explored the upstream component of JNK activation and found that IFN-α induced PKC-δ signaling caused JNK activation, resulting in induction of TRAIL-mediated apoptosis. In addition, the IFN-α-induced PKC-δ signaling was also involved in Stat1 phosphorylation on serine 727, which may also affect the cell phenotype. These observations would be useful for the development of new treatment modalities of cancer patients with IFN-α.
Human Daudi B lymphoma and U266 myeloma cells were obtained from American Type Culture Collection (Manassas, VA, USA) and were maintained at 37°C in 5% CO2 in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 50 μM 2-mercaptoethanol, and 100 μg/ml kanamycin. The cells were cultured with or without PegIFN-alpha2a (IFN-α; 1.67 × 104 U/μg: Hoffmann-LaRoche, Basel, Switzerland) in the presence or absence of PKC-δ inhibitor rottlerin (Merck Millipore, Tokyo, Japan) for the indicated times. Cell lines overexpressing dnPKC-δ (K376A), caPKC-δ (DR144/145A), or the control vector alone  were established, according to the procedure previously described .
Western blot analysis
Western blotting was performed as previously described . Briefly, cells were solubilized in lysis buffer, and equivalent amounts of protein were separated on 8.5% SDS-PAGE, followed by transfer to a PVDF membrane. After several washes, the membranes were incubated with primary Abs: mouse anti-PKC-δ monoclonal Ab (mAb) (BD Transduction Labs., San Jose, CA, USA), mouse anti-Flag mAb (Sigma, St. Louis, MO, USA), rabbit anti-JNK1 Ab, rabbit anti-STAT1 Ab (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), rabbit anti-phospho-PKC-δ (Thr505) Ab, rabbit anti-phospho-STAT1 (Tyr701) Ab (Cell Signalling Technology, Denver, MA,, USA), rabbit anti-phospho-STAT1 (Ser727) Ab (Merck Millipore), mouse anti-phospho-JNKs (Thy183/Tyr185) mAb, and rabbit anti-actin Ab (Sigma, St. Louis, MO, USA). The bound primary Abs were then incubated with horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG Ab or HRP-labeled goat anti-mouse IgG Ab (Cappel MP Biochemical, Aurora, OH, USA). The membrane-bound HRP-conjugated Ab was visualized by enhanced chemiluminescence according to the manufacturer's recommendations (Amersham Life Science, Buckinghamshire, UK). The density of each band was measured with a densitometer (Image Master DTS, Amersham Pharmacia Biotech).
Flow cytometric analysis of mitochondrial membrane potential and propidium iodide staining
The cells were stimulated with IFN-α for the indicated periods, and were assayed for surface expression of TRAIL, cells with ΔΨmlow, and PI positive cells by flow cytometry, as previously described .
Lysates from IFN-α-stimulated cells were separated into the cytosolic and nuclear fractions using Nuclear/Cytosol Fractionation Kit (Bio Vision, Mountain View, CA, USA).
Transient transfection assay
TRAIL promoter activity was determined by a luciferase reporter vector containing a TRAIL promoter fragment, as previously described . Briefly, cells were transfected with 4.5 μg reporter constructs together with 0.5 μg pRL-CMV-Renilla luciferase vector (Toyo Inki) using a Gene Pulser according to the manufacturer's instruction (Lonza Cologne, Koeln, Germany). After incubation with medium alone for 24 h, cells were stimulated with or without 1,000 U/ml IFN-α for an additional 24 h. In some experiments caPKC-δ-expressing cells were stimulated with IFN-α in the presence or absence of JNK inhibitor SP-600125 (Calbiochem, Darmstadt, Germany). The cells were assayed for luciferase activity with a Luciferase Reporter Assay System (Promega Co., Madison, WI, USA).
Data are expressed as the mean ± SD for each group. Statistical significance was determined by Student's t- test.
We thank Professor Shigeo Ohno (Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama, Japan) and Chugai Pharmaceutical Co. Ltd. (Tokyo, Japan) for supplying the PKC expression constructs and PegIFN-alpha2a, respectively. This work was supported in part by grants from the Intractable Immunological Disease Research Center at Tokyo Medical University.
- Gutterman JU: Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci USA. 1994, 91: 1198-1205. 10.1073/pnas.91.4.1198.PubMed CentralView ArticlePubMedGoogle Scholar
- Gresser I: Wherefore interferon?. J Leukoc Biol. 1997, 61: 567-574.PubMedGoogle Scholar
- Sangfelt O, Erickson S, Castro J, Heiden T, Einhorn S, Grander D: Induction of apoptosis and inhibition of cell growth are independent responses to interferon-alpha in hematopoietic cell lines. Cell Growth Differ. 1997, 8: 343-352.PubMedGoogle Scholar
- Yanase N, Takada E, Yoshihama I, Ikegami H, Mizuguchi J: Participation of Bax-alpha in IFN-alpha-mediated apoptosis in Daudi B lymphoma cells. J Interferon Cytokine Res. 1998, 18: 855-861. 10.1089/jir.1998.18.855.View ArticlePubMedGoogle Scholar
- Caraglia M, Abbruzzese A, Leardi A, Pepe S, Budillon A, Baldassare G, Selleri C, Lorenzo SD, Fabbrocini A, Giuberti G, Vitale G, Lupoli G, Bianco AR, Tagliaferri P: Interferon-alpha induces apoptosis in human KB cells through a stress-dependent mitogen activated protein kinase pathway that is antagonized by epidermal growth factor. Cell Death Differ. 1999, 6: 773-780. 10.1038/sj.cdd.4400550.View ArticlePubMedGoogle Scholar
- Grander D: How does interferon-alpha exert its antitumour activity in multiple myeloma?. Acta Oncol. 2000, 39: 801-805. 10.1080/028418600750063532.View ArticlePubMedGoogle Scholar
- Stark GR, Kerr IM, Williams BR, Silverman RH, Schreiber RD: How cells respond to interferons. Annu Rev Biochem. 1998, 67: 227-264. 10.1146/annurev.biochem.67.1.227.View ArticlePubMedGoogle Scholar
- Kotenko SV, Pestka S: Jak-Stat signal transduction pathway through the eyes of cytokine class II receptor complexes. Oncogene. 2000, 19: 2557-2565. 10.1038/sj.onc.1203524.View ArticlePubMedGoogle Scholar
- David M, Petricoin E, Benjamin C, Pine R, Weber MJ, Larner AC: Requirement for MAP kinase (ERK2) activity in interferon alpha- and interferon beta-stimulated gene expression through STAT proteins. Science. 1995, 269: 1721-1723. 10.1126/science.7569900.View ArticlePubMedGoogle Scholar
- Chang L, Karin M: Mammalian MAP kinase signalling cascades. Nature. 2001, 410: 37-40. 10.1038/35065000.View ArticlePubMedGoogle Scholar
- Platanias LC: Mechanisms of type-I- and type-II-interferon-mediated signalling. Nat Rev Immunol. 2005, 5: 375-386. 10.1038/nri1604.View ArticlePubMedGoogle Scholar
- Yanase N, Hata K, Shimo K, Hayashida M, Evers BM, Mizuguchi J: Requirement of c-Jun NH2-terminal kinase activation in interferon-alpha-induced apoptosis through upregulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in Daudi B lymphoma cells. Exp Cell Res. 2005, 310: 10-21. 10.1016/j.yexcr.2005.06.021.View ArticlePubMedGoogle Scholar
- Goh KC, Haque SJ, Williams BR: p38 MAP kinase is required for STAT1 serine phosphorylation and transcriptional activation induced by interferons. EMBO J. 1999, 18: 5601-5608. 10.1093/emboj/18.20.5601.PubMed CentralView ArticlePubMedGoogle Scholar
- Mayer IA, Verma A, Grumbach IM, Uddin S, Lekmine F, Ravandi F, Majchrzak B, Fujita S, Fish EN, Platanias LC: The p38 MAPK pathway mediates the growth inhibitory effects of interferon-alpha in BCR-ABL-expressing cells. J Biol Chem. 2001, 276: 28570-28577. 10.1074/jbc.M011685200.View ArticlePubMedGoogle Scholar
- Brodie C, Blumberg PM: Regulation of cell apoptosis by protein kinase c delta. Apoptosis. 2003, 8: 19-27. 10.1023/A:1021640817208.View ArticlePubMedGoogle Scholar
- Ohno S, Nishizuka Y: Protein kinase C isotypes and their specific functions: prologue. J Biochem (Tokyo). 2002, 132: 509-511. 10.1093/oxfordjournals.jbchem.a003249.View ArticleGoogle Scholar
- Spitaler M, Cantrell DA: Protein kinase C and beyond. Nat Immunol. 2004, 5: 785-790. 10.1038/ni1097.View ArticlePubMedGoogle Scholar
- Yoshida K, Miki Y, Kufe D: Activation of SAPK/JNK signaling by protein kinase Cdelta in response to DNA damage. J Biol Chem. 2002, 277: 48372-48378. 10.1074/jbc.M205485200.View ArticlePubMedGoogle Scholar
- Uddin S, Sassano A, Deb DK, Verma A, Majchrzak B, Rahman A, Malik AB, Fish EN, Platanias LC: Protein kinase C-delta (PKC-delta) is activated by type I interferons and mediates phosphorylation of Stat1 on serine 727. J Biol Chem. 2002, 277: 14408-14416. 10.1074/jbc.M109671200.View ArticlePubMedGoogle Scholar
- Mitsutake N, Namba H, Shklyaev SS, Tsukazaki T, Ohtsuru A, Ohba M, Kuroki T, Ayabe H, Yamashita S: PKC delta mediates ionizing radiation-induced activation of c-Jun NH(2)-terminal kinase through MKK7 in human thyroid cells. Oncogene. 2001, 20: 989-996. 10.1038/sj.onc.1204179.View ArticlePubMedGoogle Scholar
- Kaur S, Parmar S, Smith J, Katsoulidis E, Li Y, Sassano A, Majchrzak B, Uddin S, Tallman MS, Fish EN, Platanias LC: Role of protein kinase C-delta (PKC-delta) in the generation of the effects of IFN-alpha in chronic myelogenous leukemia cells. Exp Hematol. 2005, 33: 550-557. 10.1016/j.exphem.2005.01.014.View ArticlePubMedGoogle Scholar
- Jackson DN, Foster DA: The enigmatic protein kinase Cdelta: complex roles in cell proliferation and survival. FASEB J. 2004, 18: 627-636. 10.1096/fj.03-0979rev.View ArticlePubMedGoogle Scholar
- Saijo K, Mecklenbrauker I, Schmedt C, Tarakhovsky A: B cell immunity regulated by the protein kinase C family. Ann N Y Acad Sci. 2003, 987: 125-134. 10.1111/j.1749-6632.2003.tb06040.x.View ArticlePubMedGoogle Scholar
- Soltoff SP: Rottlerin is a mitochondrial uncoupler that decreases cellular ATP levels and indirectly blocks protein kinase Cdelta tyrosine phosphorylation. J Biol Chem. 2001, 276: 37986-37992.PubMedGoogle Scholar
- DeVries TA, Kalkofen RL, Matassa AA, Reyland ME: Protein kinase Cdelta regulates apoptosis via activation of STAT1. J Biol Chem. 2004, 279: 45603-45612. 10.1074/jbc.M407448200.View ArticlePubMedGoogle Scholar
- Samuel CE: Antiviral actions of interferons. Clin Microbiol Rev. 2001, 14: 778-809. 10.1128/CMR.14.4.778-809.2001. table of contentsPubMed CentralView ArticlePubMedGoogle Scholar
- Stetson DB, Medzhitov R: Type I interferons in host defense. Immunity. 2006, 25: 373-381. 10.1016/j.immuni.2006.08.007.View ArticlePubMedGoogle Scholar
- Barber GN: Host defense, viruses and apoptosis. Cell Death Differ. 2001, 8: 113-126. 10.1038/sj.cdd.4400823.View ArticlePubMedGoogle Scholar
- Le Page C, Genin P, Baines MG, Hiscott J: Interferon activation and innate immunity. Rev Immunogenet. 2000, 2: 374-386.PubMedGoogle Scholar
- Kanetaka Y, Hayashida M, Hoshika A, Yanase N, Mizuguchi J: Interferon-alpha induces transient upregulation of c-FLIP through NF-kappaB activation. Exp Cell Res. 2008, 314: 246-254. 10.1016/j.yexcr.2007.10.001.View ArticlePubMedGoogle Scholar
- Hersey P, Zhang XD: How melanoma cells evade trail-induced apoptosis. Nature Rev Cancer. 2001, 1: 142-150. 10.1038/35101078.View ArticleGoogle Scholar
- Liao YF, Hung YC, Chang WH, Tsay GJ, Hour TC, Hung HC, Liu GY: The PKC delta inhibitor, rottlerin, induces apoptosis of haematopoietic cell lines through mitochondrial membrane depolarization and caspases' cascade. Life Sci. 2005, 77: 707-719. 10.1016/j.lfs.2005.01.010.View ArticlePubMedGoogle Scholar
- Ndebele K, Gona P, Jin TG, Benhaga N, Chalah A, Degli-Esposti M, Khosravi-Far R: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induced mitochondrial pathway to apoptosis and caspase activation is potentiated by phospholipid scramblase-3. Apoptosis. 2008, 13: 845-856. 10.1007/s10495-008-0219-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Yanase N, Ohshima K, Ikegami H, Mizuguchi J: Cytochrome c release, mitochondrial membrane depolarization, caspase-3 activation, and Bax-alpha cleavage during IFN-alpha-induced apoptosis in Daudi B lymphoma cells. J Interferon Cytokine Res. 2000, 20: 1121-1129. 10.1089/107999000750053799.View ArticlePubMedGoogle Scholar
- Toyota H, Yanase N, Yoshimoto T, Moriyama M, Sudo T, Mizuguchi J: Calpain-induced Bax-cleavage product is a more potent inducer of apoptotic cell death than wild-type Bax. Cancer Lett. 2003, 189: 221-230. 10.1016/S0304-3835(02)00552-9.View ArticlePubMedGoogle Scholar
- Yanase N, Kanetaka Y, Mizuguchi J: Interferon-alpha-induced apoptosis via tumor necrosis factor-inducing ligand (TRAIL)-dependent and -independent manner. Oncol Rep. 2007, 18: 1031-1038.PubMedGoogle Scholar
- Sato K, Hida S, Takayanagi H, Yokochi T, Kayagaki N, Takeda K, Yagita H, Okumura K, Tanaka N, Taniguchi T, Ogasawara K: Antiviral response by natural killer cells through TRAIL gene induction by IFN-alpha/beta. Eur J Immunol. 2001, 31: 3138-3146. 10.1002/1521-4141(200111)31:11<3138::AID-IMMU3138>3.0.CO;2-B.View ArticlePubMedGoogle Scholar
- Wang Q, Ji Y, Wang X, Evers BM: Isolation and molecular characterization of the 5'-upstream region of the human TRAIL gene. Biochem Biophys Res Commun. 2000, 276: 466-471. 10.1006/bbrc.2000.3512.View ArticlePubMedGoogle Scholar
- Gong B, Almasan A: Genomic organization and transcriptional regulation of human Apo2/TRAIL gene. Biochem Biophys Res Commun. 2000, 278: 747-752. 10.1006/bbrc.2000.3872.View ArticlePubMedGoogle Scholar
- Kumar A, Commane M, Flickinger TW, Horvath CM, Stark GR: Defective TNF-alpha-induced apoptosis in STAT1-null cells due to low constitutive levels of caspases. Science. 1997, 278: 1630-1632. 10.1126/science.278.5343.1630.View ArticlePubMedGoogle Scholar
- Terui K, Haga S, Enosawa S, Ohnuma N, Ozaki M: Hypoxia/re-oxygenation-induced, redox-dependent activation of STAT1 (signal transducer and activator of transcription 1) confers resistance to apoptotic cell death via hsp70 induction. Biochem J. 2004, 380: 203-209. 10.1042/BJ20031891.PubMed CentralView ArticlePubMedGoogle Scholar
- Timofeeva OA, Plisov S, Evseev AA, Peng S, Jose-Kampfner M, Lovvorn HN, Dome JS, Perantoni AO: Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Oncogene. 2006, 25: 7555-7564. 10.1038/sj.onc.1209742.View ArticlePubMedGoogle Scholar
- Kaul S, Anantharam V, Yang Y, Choi CJ, Kanthasamy A, Kanthasamy AG: Tyrosine phosphorylation regulates the proteolytic activation of protein kinase Cdelta in dopaminergic neuronal cells. J Biol Chem. 2005, 280: 28721-28730. 10.1074/jbc.M501092200.View ArticlePubMedGoogle Scholar
- Joseloff E, Cataisson C, Aamodt H, Ocheni H, Blumberg P, Kraker AJ, Yuspa SH: Src family kinases phosphorylate protein kinase C delta on tyrosine residues and modify the neoplastic phenotype of skin keratinocytes. J Biol Chem. 2002, 277: 12318-12323. 10.1074/jbc.M111618200.View ArticlePubMedGoogle Scholar
- Le Good JA, Ziegler WH, Parekh DB, Alessi DR, Cohen P, Parker PJ: Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1. Science. 1998, 281: 2042-2045.View ArticlePubMedGoogle Scholar
- Bracci L, Proietti E, Belardelli F: IFN-alpha and novel strategies of combination therapy for cancer. Ann N Y Acad Sci. 2007, 1112: 256-268. 10.1196/annals.1415.030.View ArticlePubMedGoogle Scholar
- Cooper MR: A review of the clinical studies of alpha-interferon in the management of multiple myeloma. Semin Oncol. 1991, 18: 18-29.PubMedGoogle Scholar
- Hirai S, Izumi Y, Higa K, Kaibuchi K, Mizuno K, Osada S, Suzuki K, Ohno S: Ras-dependent signal transduction is indispensable but not sufficient for the activation of AP1/Jun by PKC delta. EMBO J. 1994, 13: 2331-2340.PubMed CentralPubMedGoogle Scholar
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