Regulation of DNA synthesis and the cell cycle in human prostate cancer cells and lymphocytes by ovine uterine serpin
© Padua and Hansen; licensee BioMed Central Ltd. 2008
Received: 17 August 2007
Accepted: 24 January 2008
Published: 24 January 2008
Uterine serpins are members of the serine proteinase inhibitor superfamily. Like some other serpins, these proteins do not appear to be functional proteinase inhibitors. The most studied member of the group, ovine uterine serpin (OvUS), inhibits proliferation of several cell types including activated lymphocytes, bovine preimplantation embryos, and cell lines for lymphoma, canine primary osteosarcoma and human prostate cancer (PC-3) cells. The goal for the present study was to evaluate the mechanism by which OvUS inhibits cell proliferation. In particular, it was tested whether inhibition of DNA synthesis in PC-3 cells involves cytotoxic actions of OvUS or the induction of apoptosis. The effect of OvUS in the production of the autocrine and angiogenic cytokine interleukin (IL)-8 by PC-3 cells was also determined. Finally, it was tested whether OvUS blocks specific steps in the cell cycle using both PC-3 cells and lymphocytes.
Recombinant OvUS blocked proliferation of PC-3 cells at concentrations as low as 8 μg/ml as determined by measurements of [3H]thymidine incorporation or ATP content per well. Treatment of PC-3 cells with OvUS did not cause cytotoxicity or apoptosis or alter interleukin-8 secretion into medium. Results from flow cytometry experiments showed that OvUS blocked the entry of PC-3 cells into S phase and the exit from G2/M phase. In addition, OvUS blocked entry of lymphocytes into S phase following activation of proliferation with phytohemagglutinin.
Results indicate that OvUS acts to block cell proliferation through disruption of the cell cycle dynamics rather than induction of cytotoxicity or apoptosis. The finding that OvUS can regulate cell proliferation makes this one of only a few serpins that function to inhibit cell growth.
Ser ine p roteinase in hibitors (serpins) inactivate their target proteinases through a suicide substrate-like inhibitory mechanism. The proteinase binds covalently to the reactive center loop (RCL) of the serpin and cleaves the scissile bond at the P1-P1' site. The RCL then moves to the opposite side to form the β-sheet A and a distortion in the structure of the proteinase that results in its inactivation [1–3]. Not all serpins, however, exert proteinase inhibitory activity. Some examples are corticosteroid and thyroxine binding globulins, which function as hormone transport proteins , the chaperone heat shock protein 47 , mammary serine protease inhibitor (Maspin), which increases the sensitivity of cancer cells to undergo apoptosis , and pigment epithelium derived factor (PEDF), which has neurotrophic, neuroprotective, antiangiogenic, and proapoptotic actions .
Another class of serpins without apparent proteinase activity is the uterine serpins. These proteins, which are produced by the endometrial epithelium of the pregnant cow, sow, sheep, and goat [8–13], have been classified as either a separate clade of the serpin superfamily  or as a highly-diverge group of the α1-antitrypsin clade . The best characterized protein of this unique group of serpins is ovine uterine serpin (OvUS). This basic glycoprotein is a weak inhibitor of aspartic proteinases (pepsin A and C) [12, 15], but it does not inhibit a broad range of serine proteinases [9, 16]. Additionally, amino acids in the hinge region of inhibitory serpins are not conserved in uterine serpins and OvUS behaves different in the presence of guanidine HCl than for inhibitory serpins [13, 15].
The biological function of OvUS during pregnancy may be to inhibit immune cell proliferation during pregnancy and provide protection for the allogeneically-distinct conceptus . Ovine US decreases proliferation of lymphocytes stimulated with concanavalin A, phytohemagglutinin (PHA), Candida albicans, and the mixed lymphocyte reaction [18–22]. In addition, OvUS decreases natural killer cell cytotoxic activity, abortion induced by poly(I)poly(C) in mice  and the production of antibody in sheep immunized with ovalbumin . The antiproliferative actions of OvUS are not limited to lymphocytes. Ovine US decreases development of the bovine embryos and proliferation of mouse lymphoma, canine primary osteogenic sarcoma and human prostate cancer cell lines [24, 25].
The mechanism by which OvUS inhibits proliferation of cells is unknown. The protein could block activation of cell proliferation, inhibit the cell cycle at other points or induce apoptosis or other forms of cell death. For the PC-3 prostate cancer line, inhibition of cell proliferation by OvUS might involve reduction in interleukin-8 (IL-8) secretion because of the importance of autosecretion of this cytokine for cell androgen-independent proliferation . The goal of the present study was to evaluate the mechanism by which OvUS inhibits cell proliferation. Using PC-3 cells as a model system, it was tested whether inhibition of DNA synthesis involves cytotoxic action of OvUS, induction of apoptosis or disruption of the IL-8 autocrine loop. It was also tested whether OvUS blocks specific steps in the cell cycle for PC-3 cells and lymphocytes.
Results and Discussion
Proliferation of PC-3 cells
Lactate dehydrogenase release
DNA fragmentation (apoptosis)
Cell cycle dynamics
These results indicate that OvUS block cell proliferation through cell cycle arrest in both PC-3 cells and lymphocytes. The differences in specific stages at which the cell cycle was blocked between PC-3 cells and lymphocytes is likely to be caused by differences in activation and regulation pathways for these two cell types. Unlike PC-3 cells, lymphocytes are arrested at G0 until proliferation is induced by addition of PHA. Inhibition at points in the cell cycle other than G0/G1 are less likely to be seen since few cells progress to later stages of the cell cycle. In addition, it is possible that genetic mutations in PC-3 cells compromise some potential regulatory mechanisms. In particular, unlike lymphocytes, PC-3 cells lack functional p53  which causes cell cycle arrest at G1/S by inducing p21cip1 that inhibits cyclin dependent kinases [29, 30].
The mechanism by which OvUS inhibits cell cycle dynamics is not understood. One serpin has been identified which can affect cell cycle regulatory proteins. This serpin, myeloid and erythroid nuclear termination stage-specific protein (MENT), is a nuclear protein that inhibits cell proliferation through interactions with a nuclear protein with papain-like cysteine proteinase activity . Inhibition of the proteinase prevents degradation of the cell cycle protein Rb although antiproliferative effects may depend more on other actions of MENT to mediate euchromatin condensation in an Rb-independent manner . In any case, OvUS, is apparently without proteinase inhibitory activity and is an extracellular protein that is unlikely to achieve a nuclear localization. The antipepsin activity of uterine serpin is probably not biologically significant. OvUS is a very weak inhibitor of pepsin C (a 35 and 8 fold molar excess of OvUS was required to inhibit pepsin A and C ) C and the binding of OvUS to pepsin is electrostatic . Moreover, pepsin shows an acidic pH optimum and is unlikely to be involved in cell proliferation under the conditions utilized.
A major point in the cell cycle regulated by OvUS is transition from G0/G1 to S phase: rOvUS decreased the proportion of cells in S phase in all experiments and increased the proportion of cells in G0/G1 at 24 h after treatment for PC-3 cells and at both times examined for lymphocytes. Ovine uterine serpin can bind to cell membranes  and, perhaps, OvUS inhibits proliferation by activation of signal transduction systems that inhibit transition from G0/G1 to S phase or prevents pro-proliferative molecules in culture medium from binding their receptors. Experiments with Rp-8-Cl-cAMPS, a selective inhibitor of cAMP-dependent type-I protein kinase A, indicated that effects of OvUS on proliferation of PHA-stimulated lymphocytes are not dependent on this kinase . Studies to determine activation of other anti-proliferative signal transduction systems by OvUS are warranted.
The present study indicates that the mechanism by which OvUS inhibits proliferation of PC-3 cells and lymphocytes involves cell cycle arrest and not, at least for PC-3 cells, apoptosis, cytotoxicity or inhibition of IL-8 secretion.
The human prostate cancer (PC-3) cell line was purchased from ATCC (Rockville, MD), the FreeStyle™ 293 expression medium, Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) and 0.25% Trypsin-EDTA were obtained from Gibco-Invitrogen (Carlsbad, CA), the RQ1 RNase-free DNase and the CellTiter-Glo® Luminescent Cell Viability Assay kit were obtained from Promega, (Madison, WI), the DHL™ Cell Cytotoxicity Assay kit was from Anaspec (San Jose, CA) and the ELISA MAX™ Set Deluxe kit for human IL-8 was obtained from BioLegend (San Diego, CA). The in situ cell death detection kit [terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)] was purchased from Roche (Indianapolis, IN), the DNase-free RNase A was obtained from Qiagen (Valencia, CA), Precast Tris-HCl gradient Ready gels® were from BioRad (Richmond, CA) and [3H]thymidine (6.7 Ci/mmol) was from ICN (Irvine, CA). The Prolong Antifade® kit was purchased from Molecular Probes (Eugene, OR), Geneticin was from Research products international (Mount Prospect, IL), Centricon filter devices were from Millipore Corporation (Bedford, TX), niquel Sepharose chromatography medium (high performance) was from Amersham Biosciences (Piscataway, NJ), fetal bovine and horse serum from Atlanta Biologicals (Norcross, GA). Other reagents were obtained from either Fisher (Pittsburg, PA) or Sigma-Aldrich (St. Louis, MO).
Purification of rOvUS
The His-tagged rOvUS was purified from conditioned medium of FreeStyle™ human embryonic kidney (HEK)-293F cells (Gibco-Invitrogen, Carlsbad, CA) transfected with a plasmid construct containing the gene for OvUS. Details of the cell line are provided elsewhere . Cells were cultured continuously in selective medium [FreeStyle™ 293 expression medium containing 700 μg/ml of Geneticin®] at 37°C in a humidified 8% (v/v) CO2 incubator according to the manufacturer's recommendations. Conditioned medium containing rOvUS was diluted 1:1 (v/v) in binding buffer [20 mM sodium phosphate buffer, 35 mM imidazole, 0.3 M NaCl, pH 8.0] and loaded into a nickel Sepharose column that was pre-equilibrated with binding buffer. The His-tagged rOvUS was eluted with 20 mM phosphate buffer, 500 mM imidazole, 0.3 M NaCl, pH 8.0, concentrated and buffer-exchanged into Dulbecco's phosphate buffered saline (DPBS) using Centricon plus-20 concentration devices. Purity of the rOvUS was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using precast 4–15% polyacrylamide Tris-HCl gradient gels. The protein concentration was determined by Bradford assay  using bovine serum albumin as standard.
For each experiment, rOvUS and the control protein, OVA, were added to culture wells of PC-3 cells or lymphocytes dissolved in DPBS. The vehicle control included addition of DPBS at the same volume as for rOvUS and OVA. The actual volume of protein or vehicle added varied between experiments but was generally 14–25 μl and never more than 50 μl. Cultures were set up so that the volume of DPBS was the same in all wells.
PC-3 cell culture
The PC-3 cell line was cultured continuously in Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM-F12) supplemented with 10% (v/v) heat-inactivated fetal bovine serum, 200 U/ml penicillin and 2 mg/ml streptomycin at 37°C in a humidified 5% (v/v) CO2 incubator. For the IL-8 experiment only, the medium was modified to reduce the fetal bovine serum concentration to 4% (v/v). For all the experiments, cells were cultured in 75 cm2 flasks until they reached 50–70% of confluence. Cells were then trypsinized, centrifuged at 110 × g for 5 min and resuspended in fresh complete medium. Cell viability was assessed by trypan blue exclusion and cell concentration was adjusted according to the requirements of each experiment.
[3H]Thymidine incorporation by PC-3 cells
PC-3 cells (100 μl) were plated overnight at a final concentration of 1 × 105 cell/ml in a 96-well plate. Afterwards, various concentrations of rOvUS (0, 0.5, 1, 2, 4, 8, 16, 32, 64, 125 and 250 μg/ml) or vehicle were added to each well in a total volume (including additional culture medium) of 200 μl. After 48 h of culture, 0.1 μCi [3H]thymidine in 10 μl of culture medium were added. Cells were harvested 24 h after [3H]thymidine addition onto fiber-glass filters using a cell harvester (Brandel, Gaithersburg, MD). Filters were counted for radioactivity using scintillation spectrometry (Beckman Coulter Inc., Fullerton, CA). Each concentration of protein was tested in triplicate and the experiment was performed in six different replicates using a different batch of rOvUS for each replicate.
Cell proliferation based on ATP content
Aliquots of 50 μl of PC-3 cells (1 × 105 cells/ml) were cultured for 24 h in a dark wall-clear bottom 96 well plate. Then, treatments consisting of vehicle (DPBS) or three different concentrations (50, 100 and 200 μg/ml) of rOvUS or a control protein (OVA) and culture medium added to bring the final volume to 100 μl. Additional control wells without cells were prepared to determine background. At 48 h after addition of treatments, ATP content per well was determined using the CellTiter-Glo® Luminescent Cell Viability Assay kit according to the manufacturer's instructions. Briefly, 100 μl of the CellTiter-Glo® reagent were added to each well, contents of the plate were mixed on a shaker for 2 min and then incubated at room temperature for 10 min. Chemiluminescence was quantified using a multi-detection microplate reader (FLX-800, BioTek, Winooski, VT). All treatments were performed in triplicates and the assay was performed on three different occasions using a different batch of rOvUS for each replicate.
The assay was based on the release of lactate dehydrogenase into culture medium following loss of cell membrane integrity accompanying cell death . Procedures for cell culture and treatments were similar to those described for the ATP assay. At 48 h after addition of the treatments, release of lactate dehydrogenase into the medium was determined using the DHL™ Cell Cytotoxicity Assay kit following the vendor's instructions. Briefly, the plate was equilibrated at room temperature for 20 min before adding 10 μl of lysis solution or DPBS. To facilitate cell lysing, the plate was placed on a shaker for 2 min. A total of 50 μl lactate dehydrogenase assay solution was then added to each well. After 10 min at room temperature, the reaction was stopped using 20 μl of the stop solution and the fluorescence intensity was measured using a multi-detection microplate reader (FLX-800) with excitation and emission wavelengths of 530–560 nm and 590 nm, respectively. Percent cytotoxicity was calculated by dividing 100 × fluorescence from the unlysed cells by fluorescence of the lysed cells. For each assay, each treatment was performed in six wells. The assay was replicated five different times using a different batch of rOvUS for each replicate.
An aliquot of 100 μl of PC-3 cells were cultured overnight in chamber slides at a final concentration of 1 × 104 cells/ml. Then, treatments consisting of vehicle (DPBS), 50, 100 or 200 μg/ml rOvUS, or 200 μg/ml OVA were added and additional culture medium added to produce a final volume of 300 μl. After 24 and 48 h in culture with treatments, cells were washed with PBS/PVP [100 mM sodium phosphate pH 7.4, 0.9% (w/v) NaCl, 1 mg/ml polyvinyl pyrrolidone] and fixed with 4% (w/v) paraformaldehyde for 1 h at room temperature. Cells then were washed in PBS/PVP and stored at 4°C for the TUNEL (terminal deoxynucleotidyl transferase and fluorescein isothiocyanate-conjugated dUTP nick end labeling) procedure.
For TUNEL labeling, fixed cells were incubated for 1 h at room temperature with permeabilization solution [PBS, pH 7.4, 0.1 (v/v) Triton X-100, 0.1% (w/v) sodium citrate). After washing with PBS/PVP, slides were incubated with 50 μl of TUNEL reaction mixture containing terminal deoxynucleotidyl transferase and fluorescein isothiocyanate-conjugated dUTP, for 1 hour at 37°C. Positive controls were preincubated with RQ1 RNase-free DNase (50 U/ml) and negative controls were incubated without transferase. Slides were washed with PBS/PVP, incubated for 1 h with 50 μg/ml of RNase A and then for 30 min with propidium iodide (2.5 μg/ml) at room temperature. Slides were washed with PBS/PVP and Prolong Antifade® was used to mount coverslips. Samples were observed using a Zeiss Axioplan 2 fluorescence microscope with dual filter (Carl Zeiss, Inc., Göttingen, Germany). Percent of cells with DNA fragmentation was determined by counting the total number of nuclei and total number of TUNEL-labeled nuclei at 10 different sites on the slide. The experiment was performed using three different batches of rOvUS.
Secretion of IL-8
PC-3 cells (100 μl) were cultured in wells of a 96-well plate overnight at a final concentration of 1 × 105 cells/ml. Treatments were then added including vehicle (DPBS, similar volume as for rOvUS and OVA treatments), and three different concentrations of rOvUS and OVA (50, 100 and 200 μg/ml). The volume of each well was brought to 200 μl with culture medium. At 48 h after addition of treatments, cell culture supernatants were collected, centrifuged and stored at -20°C until ELISA for IL-8. Treatments were performed in triplicate for each assay; the experiment was repeated on three different occasions using three different batches of the recombinant protein. For the measurement of IL-8, the ELISA MAX™ Set Deluxe kit for human IL-8 was used according to the manufacturer's instructions using 100 μl of conditioned medium.
Cell cycle analysis
PC-3 cells (100 μl) were cultured in 4 well plates at a final concentration of 4 × 105 cells/ml. After 24 h, treatments consisting of vehicle, 100 and 200 μg/ml rOvUS, and 200 μg/ml OVA were added with additional culture medium for a total volume of 400 μl. At 12 and 24 h after addition of treatments, cells were collected by trypsinization and washed with DPBS. Cells were fixed overnight in 70% (v/v) ethanol at 4°C, washed with DPBS and resuspended with 500 μl of staining solution [DPBS pH 7.4, 0.1% (v/v) Triton X-100, 0.05 mg/ml DNase-free RNase A, 50 μg/ml propidium iodide]. Cells were then analyzed by flow cytometry using a FACSort flow cytometer (Becton Dickinson, Franklin Lakes, NJ) and the red fluorescence of single events was recorded at wavelengths of 488 nm (excitation) and 600 nm (emission). Data were gated using pulse width and pulse area to exclude doublets, and the percent of cells present in each phase of the cell cycle was calculated using ModFITLT V3.1 software (Verity Software, Topsham, ME). The experiment was performed on three occasions with five different batches of rOvUS.
For the sheep lymphocyte experiment, mononuclear cells were purified by density gradient centrifugation from the buffy coat of heparinized peripheral blood collected by jugular venipuncture from non pregnant Rambouillet ewes . After removing red blood cells by incubation with red cell lysis buffer (0.01 M Tris-HCl pH 7.5 containing 8.3 g/L of ammonium chloride), cell viability was assessed by trypan blue exclusion, and concentration adjusted to 4 × 106 cells/ml. Cells were then suspended in a culture medium consisting of Tissue Culture Medium-199 containing 5% (v/v) horse serum, 200 U/ml penicillin, 0.2 mg/ml streptomycin, 2 mM glutamine and 10-5 M β-mercaptoethanol and aliquots of 100 μl cells cultured in 4 well plates in the presence or absence of 4 μg/ml PHA and with treatments of DPBS vehicle, 200 μg/ml rOvUS, and 200 μg/ml OVA. Total culture volume was 400 μl. After 72 and 96 h in culture at 37°C in a humidified 5% (v/v) CO2 incubator, lymphocytes were collected and washed with DPBS. Thereafter, lymphocytes were fixed and treated as described above. The experiment was performed separately for lymphocytes from four different sheep. Three different batches of rOvUS were tested for each sheep.
Data were analyzed by least-squares means analysis of variance using the General Linear Models Procedures of SAS (SAS System for Windows, Version 9.0; SAS Institute, Cary, NC, USA). Error terms were determined based on calculation of expected mean squares with replicate considered random and other main effects considered fixed. For the cytotoxicity and IL-8 data, orthogonal polynomial contrasts were used to determine the linear and quadratic effects of rOvUS and OVA. In other analysis, the pdiff mean separation test of SAS was used to distinguish the difference of various levels of a treatment.
Mammary serine proteinase inhibitor
Myeloid and erythroid nuclear termination stage-specific protein
Pigment epithelium derived factor
Terminal deoxynucleotidyl transferase (TdT) and fluorescein isothiocyanate-conjugated dUTP nick end labeling
The authors want to thank Neal Bensen and Steve McClellan from the Flow Cytometry Core Laboratory, Facility of the Interdisciplinary Center of Biotechnology Research, University of Florida and to Luciano Bonilla for help with the sheep. Thanks are also extended to the following University of Florida people for providing access to laboratory equipment: Dr. Owen Rae and Shelley Lanhart from the Department of Large Animal Clinical Sciences, Dr. Lori Warren and Jan Kivilpeto from the Department of Animal Sciences, and Dr. Pushpa Kalra from the Dept. of Physiology and Functional Genomics.
- Irving JA, Pike RN, Lesk AM, Whisstock JC: Phylogeny of the serpin superfamily: Implications of patterns of amino acid conservation for structure and function. Genome Res. 2000, 10: 1845-1864. 10.1101/gr.GR-1478R.View ArticlePubMed
- Van Gent D, Sharp P, Morgan K, Kalsheker N: Serpins: structure, function and molecular evolution. Int J Biochem Cell Biol. 2003, 35: 1536-1547. 10.1016/S1357-2725(03)00134-1.View ArticlePubMed
- Law RHP, Zhang Q, McGowan S, Buckle AM, Silverman GA, Wong W, Rosado CJ, Langendorf CG, Pike RN, Bird PI, Whisstock JC: An overview of the serpin superfamily. Genome Biol. 2006, 7: 216-10.1186/gb-2006-7-5-216.PubMed CentralView ArticlePubMed
- Pemberton PA, Stein PE, Pepys MB, Potter JM, Carrell RW: Hormone binding globulins undergo serpin conformational change in inflammation. Nature. 1988, 336: 257-258. 10.1038/336257a0.View ArticlePubMed
- Sauk JJ, Nikitakis N, Siavash H: Hsp47 a novel collagen binding serpin chaperone, autoantigen and therapeutic target. Front Biosci. 2005, 10: 107-118. 10.2741/1513.View ArticlePubMed
- Sheng S: A role of novel serpin maspin in tumor progression: the divergence revealed through efforts to converge. J Cell Physiol. 2006, 209: 631-635. 10.1002/jcp.20786.View ArticlePubMed
- Fernandez-Garcia NI, Volpert OV, Jimenez B: Pigment epithelium-derived factor as a multifunctional antitumor factor. J Mol Med. 2007, 85: 15-22. 10.1007/s00109-006-0111-z.View ArticlePubMed
- Moffatt J, Bazer FW, Hansen PJ, Chun PW, Roberts RM: Purification, secretion and immunocytochemical localization of the uterine milk proteins, major progesterone-induced proteins in uterine secretions of the sheep. Biol Reprod. 1987, 36: 419-430. 10.1095/biolreprod36.2.419.View ArticlePubMed
- Ing NH, Roberts RM: The major progesterone-induced proteins secreted into the sheep uterus are members of the serpin superfamily of protease inhibitors. J Biol Chem. 1989, 264: 3372-3379.PubMed
- Malathy PV, Imakawa K, Simmen RC, Roberts RM: Molecular cloning of the uteroferrin-associated protein, a major progesterone-induced serpin secreted by the porcine uterus, and expression of its mRNA during pregnancy. Mol Endocrinol. 1990, 4: 428-440.View ArticlePubMed
- Leslie MV, Hansen PJ, Newton GR: Uterine secretions of the cow contain proteins that are immunochemically related to the major progesterone-induced proteins of the sheep uterus. Domest Anim Endocrinol. 1990, 7: 517-526. 10.1016/0739-7240(90)90009-O.View ArticlePubMed
- Mathialagan N, Hansen TR: Pepsin-inhibitory activity of uterine serpins. Proc Natl Acad Sci. 1996, 93: 13653-13658. 10.1073/pnas.93.24.13653.PubMed CentralView ArticlePubMed
- Tekin S, Padua MB, Newton GR, Hansen PJ: Identification and cloning of caprine uterine serpin. Mol Reprod Dev. 2005, 70: 262-270. 10.1002/mrd.20206.View ArticlePubMed
- Peltier MR, Raley LC, Liberles DA, Benner SA, Hansen PJ: Evolutionary history of the uterine serpins. J Exp Zool. 2000, 288: 165-174. 10.1002/1097-010X(20000815)288:2<165::AID-JEZ7>3.0.CO;2-R.View ArticlePubMed
- Peltier MR, Grant TR, Hansen PJ: Distinct physical and structural properties of the ovine uterine serpin. Biochim Biophys Acta. 2000, 1479: 37-51.View ArticlePubMed
- Liu WJ, Hansen PJ: Progesterone-induced secretion of dipeptidyl peptidase-IV (cell differentiation antigen 26) by the uterine endometrium of the ewe and cow that costimulates lymphocyte proliferation. Endocrinology. 1995, 136: 779-787. 10.1210/en.136.2.779.PubMed
- Hansen PJ: Regulation of uterine immune function by progesterone-lessons from the sheep. J Reprod Immunol. 1998, 40: 63-79. 10.1016/S0165-0378(98)00035-7.View ArticlePubMed
- Segerson EC, Moffatt RJ, Bazer FW, Roberts MR: Suppression of phytohemagglutinin-stimulated lymphocyte blastogenesis by ovine uterine milk protein. Biol Reprod. 1984, 30: 1175-1186. 10.1095/biolreprod30.5.1175.View ArticlePubMed
- Hansen PJ, Segerson EC, Bazer FW: Characterization of immunosuppressive substances in the basic protein fraction of uterine secretions of pregnant ewes. Biol Reprod. 1987, 36: 393-403. 10.1095/biolreprod36.2.393.View ArticlePubMed
- Skopets B, Hansen PJ: Identification of the predominant proteins in uterine fluids of unilaterally pregnant ewes that inhibit lymphocyte proliferation. Biol Reprod. 1993, 49: 997-1007. 10.1095/biolreprod49.5.997.View ArticlePubMed
- Skopets B, Liu WJ, Hansen PJ: Effects of endometrial serpin-like proteins on immune responses in sheep. Am J Reprod Immunol. 1995, 33: 86-93.View ArticlePubMed
- Peltier MR, Liu WJ, Hansen PJ: Regulation of lymphocyte proliferation by uterine serpin: interleukin-2 mRNA production, CD25 expression and responsiveness to interleukin-2. Exp Biol Med. 1999, 223: 75-81. 10.1046/j.1525-1373.2000.22310.x.
- Liu WJ, Hansen PJ: Effect of the progesterone-induced serpin-like proteins of the sheep endometrium on natural-killer cell activity in sheep and mice. Biol Reprod. 1993, 49: 1008-1014. 10.1095/biolreprod49.5.1008.View ArticlePubMed
- Tekin S, Padua MB, Brad AM, Hansen PJ: Antiproliferative actions of ovine uterine serpin. Am J Reprod Immunol. 2005, 53: 136-143. 10.1111/j.1600-0897.2005.00257.x.View ArticlePubMed
- Tekin S, Padua MB, Brad AM, Rhodes ML, Hansen PJ: Expression and properties of recombinant ovine uterine serpin. Exp Biol Med. 2006, 231: 1313-1322.
- Araki S, Omori Y, Lyn D, Singh RK, Meinbach DM, Sandman Y, Lokeshwar VB, Lokeshwar BL: Interleukin-8 is a molecular determinant on androgen independence and progression in prostate cancer cells. Cancer Res. 2007, 67: 6854-6862. 10.1158/0008-5472.CAN-07-1162.View ArticlePubMed
- Handayani R, Rice L, Cui Y, Medrano TA, Samedi VG, Baker HV, Szabo NJ, Shiverick KT: Soy isoflavones alter expression of genes associated with cancer progression, including interleukin-8, in androgen-independent PC-3 human prostate cancer cells. J Nutr. 2006, 136: 75-82.PubMed
- Isaacs WB, Carter BS, Erwing CM: Wild-type p53 supresses growth of human prostate cancer cells containing mutant p53 alleles. Cancer Res. 1991, 51: 4716-4720.PubMed
- Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ: The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin dependent kinases. Cell. 1993, 75: 805-816. 10.1016/0092-8674(93)90499-G.View ArticlePubMed
- Duliæ V, Kaufmann WK, Wilson SJ, Tlsty TD, Lees E, Harper JW, Elledge SJ, Reed SI: p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest. Cell. 1994, 76: 1013-1023. 10.1016/0092-8674(94)90379-4.View Article
- Irving JA, Shushanov SS, Pike RN, Popova EY, Bromme D, Coetzer THT, Bottomley SP, Boulynko IA, Grigoryev SA, Whisstock JC: Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and can regulate cell proliferation. J Biol Chem. 2002, 277: 13192-13201. 10.1074/jbc.M108460200.View ArticlePubMed
- Liu WJ, Peltier MR, Hansen PJ: Binding of ovine uterine serpin to lymphocytes. Am J Reprod Immunol. 1999, 41: 428-432.View ArticlePubMed
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 78: 248-254. 10.1016/0003-2697(76)90527-3.View Article
- Decker T, Lohmann-Matthes ML: A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods. 1988, 115: 61-69. 10.1016/0022-1759(88)90310-9.View ArticlePubMed
- Tekin S, Hansen PJ: Natural-killer like cells in the sheep: Functional characterization and regulation by pregnancy-associated proteins. Exp Biol Med (Maywood). 2002, 227: 803-811.
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