Myosin-Vb functions as a dynamic tether for peripheral endocytic compartments during transferrin trafficking
© Provance et al; licensee BioMed Central Ltd. 2008
Received: 19 February 2008
Accepted: 07 August 2008
Published: 07 August 2008
Myosin-Vb has been shown to be involved in the recycling of diverse proteins in multiple cell types. Studies on transferrin trafficking in HeLa cells using a dominant-negative myosin-Vb tail fragment suggested that myosin-Vb was required for recycling from perinuclear compartments to the plasma membrane. However, chemical-genetic, dominant-negative experiments, in which myosin-Vb was specifically induced to bind to actin, suggested that the initial hypothesis was incorrect both in its site and mode of myosin-Vb action. Instead, the chemical-genetic data suggested that myosin-Vb functions in the actin-rich periphery as a dynamic tether on peripheral endosomes, retarding transferrin transport to perinuclear compartments.
In this study, we employed both approaches, with the addition of overexpression of full-length wild-type myosin-Vb and switching the order of myosin-Vb inhibition and transferrin loading, to distinguish between these hypotheses. Overexpression of full-length myosin-Vb produced large peripheral endosomes. Chemical-genetic inhibition of myosin-Vb after loading with transferrin did not prevent movement of transferrin from perinuclear compartments; however, virtually all myosin-Vb-decorated particles, including those moving on microtubules, were halted by the inhibition. Overexpression of the myosin-Vb tail caused a less-peripheral distribution of early endosome antigen-1 (EEA1).
All results favored the peripheral dynamic tethering hypothesis.
Molecular motors generally are thought to be recruited to vesicles or organelles to provide directional movement; however, this perspective is complicated by evidence showing that multiple motors, using multiple cytoskeletal substrates, are found on individual vesicles and organelles [1, 2, 2–7]. While kinesins and dyneins clearly transport cargo for long distances in vivo, there is surprisingly little evidence for such a role for unconventional myosins in higher eukaryotes . Biophysical studies have shown that many myosin head domains bind more tightly to actin in response to loading [9–12], but these adaptations usually are interpreted as promoting processive transport of cargo over long distances. However, the biophysical data also are consistent with adaptation to function as dynamic tethers or tensioners between actin filaments and other cytoplasmic structures. In this context, tethering is distinct from docking, in that it may simply represent a net balance of forces, movements, and/or positions. Accordingly, retention (and active transport) within cortical actin might prevent endosomes from encountering microtubules, so tethering may represent an effect rather than a distinct molecular mechanism. Actual point-to-point transport of cargo by unconventional myosins in a cellular context might be relatively rare; for example, to reposition the myosin in the absence or reduction of load, halting as a new load is sensed.
Myosin-Vb, originally named myr 6 , is a member of one of the most ancient divisions of the myosin superfamily , with diverse cellular functions. It interacts with the brain-expressed RING finger protein BERP, Rab11a, Rab11a-FIP2, Rab11b, Rab25, and Rab8a [15–19]. It has been implicated in recycling of transferrin and its receptor [16, 18, 20], the chemokine receptor CXC2 , HIV Vpu , acetylcholine receptors , the polymeric IgA receptor [16, 24], and the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptor subunit GluR1 . It also has been implicated in formation of bile canaliculi .
Overexpression of tail fragments of unconventional myosins has been the standard technique for their inhibition, and data from these experiments are usually interpreted in the context of point-to-point transport. For myosin-Vb in transferrin trafficking, overexpression of a tail fragment in HeLa cells caused accumulation of transferrin in perinuclear compartments, suggesting that myosin-Vb functions in the transport of vesicles between perinuclear recycling endosomes and the plasma membrane . By contrast, we adapted a chemical-genetic method pioneered by Shokat and colleagues for kinases [27, 28] to unconventional myosins, allowing us to acutely and specifically induce tight binding of a sensitized mutant myosin to actin by microinjection or dialysis of an ADP analog [29–32]. When we inhibited the sensitized mutant myosin-Vb (also dominant-negative inhibition), it prevented accumulation of transferrin-positive vesicles and organelles in the perinuclear region . This result was inconsistent with the transport hypothesis, because if myosin-Vb is required for transport between perinuclear compartments and the plasma membrane, induction of tight binding to actin should have caused transferrin to accumulate in perinuclear compartments.
These apparently contradictory results could be reconciled if myosin-Vb acts peripherally as a dynamic tether that antagonizes the retrograde transport of transferrin to perinuclear compartments, possibly by holding the parental organelle in the periphery during fission. We also observed an increase in plasma-membrane transferrin receptor upon myosin-Vb inhibition , suggesting that chemical-genetic inhibition had shunted trafficking to the rapid peripheral pathway . The tail-fragment overexpression data can be explained as release of the peripheral endocytic compartments from actin, allowing entire peripheral endosomes to be transported to the perinuclear region.
In this study, we have employed three different perturbations of myosin-Vb function to further test the dynamic tethering hypothesis, which makes clear predictions: first, overexpression of full-length, functional myosin-Vb will prevent transferrin from reaching perinuclear compartments; second, chemical-genetic inhibition of sensitized mutant myosin-Vb in cells after transferrin loading (in our previous study, it was done before transferrin loading) will neither cause accumulation of transferrin in perinuclear compartments nor prevent transferrin from moving from perinuclear compartments to the plasma membrane; and third, overexpression of the myosin-Vb tail fragment will cause at least some peripheral endocytic markers to assume more perinuclear distributions. The new data generally contradict the transport hypothesis. In addition, our data suggest that members of the myosin-V family may play a ubiquitous function in modulating vesicle transport along microtubules, as they are available to interact with passing actin filaments as passengers. Applied more broadly, our data suggest that identifying endocytic compartments by their positions within the cytoplasm may be unreliable in the context of significant experimental disruptions.
Results and Discussion
We have shown that expression of low levels of exogenous myosin-Vb (25–40% of endogenous levels) does not alter the trafficking of transferrin . However, the dynamic tethering hypothesis predicts that exceeding endogenous levels with wild-type exogenous myosin-Vb will alter the balance of forces, reducing the extent and/or rate of retrograde movement from peripheral to perinuclear compartments. To test this prediction, we increased the amount of myosin-Vb associated with those compartments by transiently transfecting HeLa cells with a full-length, wild-type myosin-Vb construct. To allow imaging of live cells, we used a construct with an N-terminal eGFP tag . We compared the distribution of eGFP-tagged myosin-Vb with that of our C-terminal -tagged (V5 and 6x-His) version , and observed no significant differences (data not shown). At low levels of eGFP-myosin-Vb expression, we observed only occasional, highly dynamic, colocalization of myosin-Vb and transferrin (arrows, Fig. 1D,E,F; Additional file 1).
HeLa cells that endocytosed fluorescent transferrin before and during overexpression of eGFP-myosin-Vb sequestered transferrin in large peripheral compartments decorated with myosin-Vb (Fig. 2D,E,F; Additional file 2), suggesting that fission of the compartments in which myosin-Vb and transferrin normally transiently colocalize (arrow, Fig. 1D,E,F; Additional file 1) was inhibited. By contrast, when transferrin was introduced after overexpression of myosin-Vb, transferrin was not colocalized with myosin-Vb in the enlarged peripheral compartments (arrows, Fig. 2F–K; Additional files 3, 4, 5). In addition, transferrin failed to accumulate in perinuclear compartments. As a negative control, we expressed a truncated myosin-Vb consisting of the head domain and first IQ domain, which had no effect on transferrin localization (data not shown). These data suggest that that overexpression of myosin-Vb prevents transferrin from both entering into and exiting from a normally dynamic, short-lived endocytic compartment.
In isolation, the static images shown in Figure 2 can be fit to the anterograde transport model if overexpression caused rapid transport of transferrin from perinuclear compartments while delaying its passage through cortical actin. However, Additional files 3, 4, 5 show that transferrin is not reaching perinuclear compartments.
As these data suggest that fission of vesicles from peripheral endocytic compartments and/or their transport to perinuclear compartments had been prevented by increased tethering to cortical actin, we examined the distribution of the endocytic markers Rab11a, Rab4, and Rab5. Cotransfections with eGFP-myosin-Vb and the recycling endosome marker mRFP-Rab11a showed virtually complete colocalization at high levels of myosin-Vb expression (Additional file 6). By contrast, little colocalization was observed in cells cotransfected with eGFP-myosin-Vb and the early endosome markers mRFP-Rab4 (Additional file 7) and mRFP-Rab5 (Additional file 8), suggesting that trafficking through early endosomes was not prevented. The videos also show that the enlarged endosomes are relatively static, consistent with increased tethering forces and caging by actin.
Additional file 10: Chemical-genetic inhibition of sensitized mutant (Y119G) eGFP-myosin-Vb by PE-ADP microinjection (cell on left) halts movement of all myosin-Vb-decorated particles, including those being transported via microtubules; same field as Figure 3A. Uninjected control cell is on the right. Frame acquisition rate, 1/sec; frame display rate, 10/sec. (MOV 8 MB)
The arrest of microtubule-based motility of myosin-Vb-decorated particles was unexpected, and we initially suspected that it might have been an artifact of high effective ADP concentration in the form of the microinjected PE-ADP analog. To test the hypothesis that myosin-Vb interacts transiently with actin filaments during microtubule-based transport under normal conditions, we measured the speeds of particles decorated with wild-type eGFP-tagged myosin-Vb before and after the addition of latrunculin A. If myosin-Vb (or other myosins) normally interacts with actin filaments, latrunculin A treatment should increase both mean speed and the proportion of vesicles moving at 0.7–1.0 μm/sec. This prediction was confirmed, as latrunculin treatment nearly doubled the proportion of particles exhibiting rapid movement (Figure 4H), in contrast with results from melanosome transport in fish melanophores . The modification of the dynamic tethering hypothesis to account for these data is diagrammed in Fig. 4I and 4J.
Based on the change in distribution of EEA1 coupled with its failure to colocalize with the myosin-Vb tail, we hypothesize that in the presence of the tail, endosomes still are transported to more perinuclear regions of the cytoplasm, but the fission between their domains that normally occurs in peripheral regions occurs in a more perinuclear location. We then confirmed the effect of the myosin-Vb tail on Rab11a redistribution. As observed by Lapierre et al., the dispersed pattern observed in untransfected control cells (Fig. 5E,F,G, arrowheads) was changed to a more perinuclear pattern by overexpression of the eGFP/myosin-Vb tail (arrows).
We next examined Rab8a, which has been shown to interact in vitro with myosin-Vb . We observed a nearly normal distribution of Rab8a despite the overexpression of the myosin-Vb tail (Fig. 5H,I,J). These results are consistent with the differences between Rab11a and Rab8a compartments and pathways observed by Roland et al., and indicate that the affinity of the myosin-Vb tail domain for Rab8a is much lower than its affinity for Rab11a. This result also is consistent with their inability to observe interaction between myosin-Vb and Rab8a in a cellular context.
To summarize our model, myosin-Vb is associated with multiple compartments, of which only some are involved in transferrin trafficking. Myosin-Vb primarily tethers a subset of peripheral, Rab11a-positive endocytic compartments to cortical actin, opposing forces from dynein or minus-end-directed kinesins and retaining the compartment in the actin-rich periphery. This is analogous to the mechanism of Velcro™, except that instead of hooks bending, the myosin-Vb heads are going through the ATPase cycle and periodically releasing from actin. In this analogy, overexpression of full-length, wild-type myosin-Vb (Fig. 2) causes greater retention of normally endocytic compartments in the periphery, leading to their coalescence, because the increase in number of myosins outweighs their individual cycling off and back onto actin. These data strongly suggest that while this compartment is normally rare and transient (Fig. 1), all transferrin still must pass through it to reach perinuclear compartments. Both our overexpression of myosin-Vb and overexpression of the myosin-Vb tail create artifacts. In the former case (Fig. 2), caging by actin causes coalescence and blockage of both entry and exit; while in the latter case , release from actin causes what we believe to be virtually the same compartment to collapse to a perinuclear location. Chemical-genetic inhibition is analogous to preventing individual Velcro™ hooks from bending, but does not prevent entry and exit into this compartment via the peripheral pathway .
Myosin-Va, the founding member of this myosin family, appears to have a similar function, albeit involving different compartments. In melanocytes, we first suggested a peripheral tethering function for myosin-Va based on the mutant phenotype, a perinuclear accumulation of melanosomes . The best-characterized system for melanosome transport has been Xenopus melanophores [4, 5, 38, 39], with similar, but less dynamic, results from murine melanocytes . In both cases, myosin-Va function was hypothesized to provide not only peripheral capture, but transport within the periphery as well. While pauses in microtubule-based movement attributed to myosin-Va have been observed [41, 42], these studies represent the first such observation for myosin-Vb. In general, myosin-Vb appears to perform the same function in the endocytic pathway as myosin-Va performs in exocytic pathways, and our future experiments will test the validity of our generalization. In a technical context, our results suggest that membrane compartments cannot necessarily be reliably identified by their locations within the cytoplasm in cells in which trafficking has been grossly perturbed by manipulation, particularly overexpression, of any relevant component.
The full-length eGFP-wild-type myosin-Vb, eGFP-myosin-Vb tail, mRFP-Rab4a, mRFP-Rab5a, mRFP-Rab8a and mRFP-Rab11a expression constructs were gifts from Jim Goldenring and are based on the peGFP-C2 expression vector (Clontech). The sensitized Y119G mutant eGFP myosin-Vb was created by shuttling a 989-bp Cla I/Bst EII fragment from pEcho/pcDNA3.1 Y119G myosin-Vb . The control eGFP-tagged myosin-Vb 1IQ expression vector was created by amplifying the eGFP-tagged, wild-type myosin-Vb sequence for the head domain through the first IQ domain and cloning into pYY8.
Cells and transfection
HeLa cells were cultured as described . For transfections, 1 μg of Lipofectamine 2000 (Invitrogen) was added to 40 μl of OptiMEM (Invitrogen), then mixed with 0.5 μg of DNA diluted into 40 μl of OptiMEM according to manufacturer's instructions. HeLa cells were trypsinized, collected and resuspended in complete medium at a concentration of 1 × 106 cells/ml. The cell suspension (80 μl) was added to the DNA/liposome mixture and plated as 40-μl dots in live-cell chambers (Bioptechs, Butler, PA) or glass coverslips, incubated for 1–2 h and then flooded with complete medium. Cells were used in experiments 24–48 h after transfection.
EEA1 was detected using a monoclonal antibody (BD Transduction Laboratories 610456). The primary antibody was detected with Alexa-546- or Alexa-647-labeled goat anti-mouse secondary (Invitrogen). For cell outlines, actin was stained with Alexa-647-labeled phallacidin (Invitrogen).
Transferrin trafficking, microinjection, and latrunculin treatment
Transfected cells were incubated in serum-free medium for 60 min, then exposed to 10 μg/ml Alexa 546-labeled transferrin (Invitrogen) for 1 min, washed 3 times with PBS and incubated in pre-equilibrated complete medium for the duration of live-cell experiments. For concomitant labeling with transferrin before expression of exogenous eGFP-myosin-Vb (Fig. 2D,E,F), fluorescent transferrin (10 μg/ml) was added to the complete medium used to flood the coverslips during transfection as described above. During live-cell experiments, stage positions of individual transfected cells were stored using MetaMorph (Molecular Devices) and a motorized stage (Prior). To inhibit Y119G myosin-Vb, HeLa cells were injected in the nucleus with 10 mM PE-ADP, 100 mM KCl, 8 mM K-phosphate (pH 7.0), 0.05 mg/ml fixable Alexa 647-labeled dextran (Invitrogen), and 10 mM Mg-ATP using a Harvard Apparatus PLI-100 at 8–15 kilopascals; negative control injections contained the same solution lacking PE-ADP. Assuming an injection volume of ~3% cell volume, the final concentration of PE-ADP was ~300 μM. To inhibit actin polymerization, latrunculin A (Molecular Probes) was added to medium at 2.5 μg/ml.
Imaging and Quantitation
All images were obtained using a Nikon TE2000E equipped with a Q57 12-bit CCD camera (Roper Scientific) controlled by MetaMorph software. Images were obtained through a 60× (1.2 NA) water-immersion lens that was maintained at 37°C using an objective heater (Bioptechs). For time-lapse movies, images were obtained at a rate of 1 frame/sec over a 1-min time course. Fluorescence imaging of the dextran to identify injected cells was performed following the time-lapse imaging. Instantaneous speeds of individual particles were measured by observers (blindly with respect to experimental conditions) using the Track Points package of MetaMorph and the data were exported to Microsoft Excel. Measurements were obtained from 5–10 cells per condition, and numbers of particles are provided in the Figure 3 legend. Since pixel size produced submicron/sec speed measurement errors, speeds were separated into 3 bins: 0–0.15 μm/sec (stationary and actin-based), 0.16–0.70 μm/sec (both actin- and microtubule-based), and 0.71–1.0 μm/sec (microtubule-based).
All videos are of HeLa cells.
early endosomal antigen-1
- and PE-ADP:
This work was funded by NIH R01 GM066901 (JAM). Core facility support was provided by P20 RR15583. Stipend support was provided by the American Cancer Society (DZC), and donations from James Wylder, W. Peter Horst, and Howard Bethel (PRW and EJA). We thank Deb Cabin, George Carlson, John Bermingham, Richard Bennett, and Mike Ehlers for critical reviews of the manuscript.
- Huang JD, Brady ST, Richards BW, Stenolen D, Resau JH, Copeland NG, Jenkins NA: Direct interaction of microtubule- and actin-based transport motors. Nature. 1999, 397: 267-270.View ArticlePubMedGoogle Scholar
- Brown SS: Cooperation between microtubule- and actin-based motor proteins. Annu Rev Cell Dev Biol. 1999, 15: 63-80.View ArticlePubMedGoogle Scholar
- Manneville JB, Etienne-Manneville S, Skehel P, Carter T, Ogden D, Ferenczi M: Interaction of the actin cytoskeleton with microtubules regulates secretory organelle movement near the plasma membrane in human endothelial cells. J Cell Sci. 2003, 116: 3927-3938.View ArticlePubMedGoogle Scholar
- Gross SP, Tuma MC, Deacon SW, Serpinskaya AS, Reilein AR, Gelfand VI: Interactions and regulation of molecular motors in Xenopus melanophores. J Cell Biol. 2002, 156: 855-865.PubMed CentralView ArticlePubMedGoogle Scholar
- Kural C, Serpinskaya AS, Chou YH, Goldman RD, Gelfand VI, Selvin PR: Tracking melanosomes inside a cell to study molecular motors and their interaction. Proc Natl Acad Sci USA. 2007, 104: 5378-5382.PubMed CentralView ArticlePubMedGoogle Scholar
- Levi V, Serpinskaya AS, Gratton E, Gelfand V: Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors. Biophys J. 2006, 90: 318-327.PubMed CentralView ArticlePubMedGoogle Scholar
- Mallik R, Gross SP: Molecular motors: strategies to get along. Curr Biol. 2004, 14: R971-82.View ArticlePubMedGoogle Scholar
- Stachelek SJ, Tuft RA, Lifschitz LM, Leonard DM, Farwell AP, Leonard JL: Real-time visualization of processive myosin 5a-mediated vesicle movement in living astrocytes. J Biol Chem. 2001, 276: 35652-35659.View ArticlePubMedGoogle Scholar
- Cremo CR, Geeves MA: Interaction of actin and ADP with the head domain of smooth muscle myosin: implications for strain-dependent ADP release in smooth muscle. Biochemistry. 1998, 37: 1969-1978.View ArticlePubMedGoogle Scholar
- Batters C, Arthur CP, Lin A, Porter J, Geeves MA, Milligan RA, Molloy JE, Coluccio LM: Myo1c is designed for the adaptation response in the inner ear. EMBO J. 2004, 23: 1433-1440.PubMed CentralView ArticlePubMedGoogle Scholar
- Veigel C, Schmitz S, Wang F, Sellers JR: Load-dependent kinetics of myosin-V can explain its high processivity. Nat Cell Biol. 2005, 7: 861-869.View ArticlePubMedGoogle Scholar
- Purcell TJ, Sweeney HL, Spudich JA: A force-dependent state controls the coordination of processive myosin V. Proc Natl Acad Sci USA. 2005, 102: 13873-13878.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhao LP, Koslovsky JS, Reinhard J, Bähler M, Witt AE, Provance DW, Mercer JA: Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family. Proc Nat Acad Sci USA. 1996, 93: 10826-10831.PubMed CentralView ArticlePubMedGoogle Scholar
- Richards TA, Cavalier-Smith T: Myosin domain evolution and the primary divergence of eukaryotes. Nature. 2005, 436: 1113-1118.View ArticlePubMedGoogle Scholar
- El-Husseini AE, Vincent SR: Cloning and characterization of a novel RING finger protein that interacts with class V myosins. J Biol Chem. 1999, 274: 19771-19777.View ArticlePubMedGoogle Scholar
- Lapierre LA, Kumar R, Hales CM, Navarre J, Bhartur SG, Burnette JO, Provance DW, Mercer JA, Bahler M, Goldenring JR: Myosin Vb is associated with plasma membrane recycling systems. Mol Biol Cell. 2001, 12: 1843-1857.PubMed CentralView ArticlePubMedGoogle Scholar
- Lindsay AJ, McCaffrey MW: Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain. J Biol Chem. 2002, 277: 27193-27199.View ArticlePubMedGoogle Scholar
- Yan Q, Sun W, Kujala P, Lotfi Y, Vida TA, Bean AJ: CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling. Mol Biol Cell. 2005, 16: 2470-2482.PubMed CentralView ArticlePubMedGoogle Scholar
- Roland JT, Kenworthy AK, Peranen J, Caplan S, Goldenring JR: Myosin Vb Interacts with Rab8a on a tubular network containing EHD1 and EHD3. Mol Biol Cell. 2007, 18: 2828-2837.PubMed CentralView ArticlePubMedGoogle Scholar
- Provance DW, Gourley CR, Silan CM, Cameron LC, Shokat KM, Goldenring JR, hah K, Gillespie PG, Mercer JA: Chemical-genetic inhibition of a sensitized mutant myosin-Vb demonstrates a role in peripheral-pericentriolar membrane traffic. Proc Natl Acad Sci USA. 2004, 101: 1868-1873.PubMed CentralView ArticlePubMedGoogle Scholar
- Fan GH, Lapierre LA, Goldenring JR, Richmond A: Differential regulation of CXCR2 trafficking by Rab GTPases. Blood. 2003, 101: 2115-2124.View ArticlePubMedGoogle Scholar
- Varthakavi V, Smith RM, Martin KL, Derdowski A, Lapierre LA, Goldenring JR, Spearman P: The pericentriolar recycling endosome plays a key role in Vpu-mediated enhancement of HIV-1 particle release. Traffic. 2006, 7: 298-307.View ArticlePubMedGoogle Scholar
- Volpicelli LA, Lah JJ, Fang G, Goldenring JR, Levey AI: Rab11a and myosin Vb regulate recycling of the M4 muscarinic acetylcholine receptor. J Neurosci. 2002, 22: 9776-9784.PubMedGoogle Scholar
- Wang X, Kumar R, Navarre J, Casanova JE, Goldenring JR: Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25. J Biol Chem. 2000, 275: 29138-29146.View ArticlePubMedGoogle Scholar
- Lisé MF, Wong TP, Trinh A, Hines RM, Liu L, Kang R, Hines DJ, Lu J, Goldenring JR, Wang YT, El-Husseini A: Involvement of myosin Vb in glutamate receptor trafficking. J Biol Chem. 2006, 281: 3669-3678.View ArticlePubMedGoogle Scholar
- Wakabayashi Y, Dutt P, Lippincott-Schwartz J, Arias IM: Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells. Proc Natl Acad Sci USA. 2005, 102: 15087-15092.PubMed CentralView ArticlePubMedGoogle Scholar
- Specht KM, Shokat KM: The emerging power of chemical genetics. Curr Opin Cell Biol. 2002, 14: 155-159.View ArticlePubMedGoogle Scholar
- Knight ZA, Shokat KM: Chemical genetics: where genetics and pharmacology meet. Cell. 2007, 128: 425-430.View ArticlePubMedGoogle Scholar
- Gillespie PG, Gillespie SKH, Mercer JA, Shah K, Shokat KM: Engineering of the myosin-I beta nucleotide-binding pocket to create selective sensitivity to N6-modified ADP analogs. Journal of Biological Chemistry. 1999, 274: 31373-31381.View ArticlePubMedGoogle Scholar
- Holt JR, Gillespie SKH, Provance DW, Shah K, Shokat KM, Corey DP, Mercer JA, Gillespie PG: A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell. 2002, 108: 371-381.View ArticlePubMedGoogle Scholar
- Stauffer EA, Scarborough JD, Hirono M, Miller ED, Shah K, Mercer JA, Holt JR, Gillespie PG: Fast adaptation in vestibular hair cells requires myosin-1c activity. Neuron. 2005, 47: 541-553.PubMed CentralView ArticlePubMedGoogle Scholar
- Karcher RL, Provance DW, Gillespie PG, Mercer JA: Chemical-genetic inhibition of sensitized mutant unconventional myosins. Methods in Molecular Biology. 2007, 392: 231-240.View ArticlePubMedGoogle Scholar
- Hao M, Maxfield FR: Characterization of rapid membrane internalization and recycling. J Biol Chem. 2000, 275: 15279-15286.View ArticlePubMedGoogle Scholar
- Rodionov V, Yi J, Kashina A, Oladipo A, Gross SP: Switching between microtubule- and actin-based transport systems in melanophores is controlled by cAMP levels. Curr Biol. 2003, 13: 1837-1847.View ArticlePubMedGoogle Scholar
- Sonnichsen B, De Renzis S, Nielsen E, Rietdorf J, Zerial M: Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11. J Cell Biol. 2000, 149: 901-914.PubMed CentralView ArticlePubMedGoogle Scholar
- Mu FT, Callaghan JM, Steelemortimer O, Stenmark H, Parton RG, Campbell PL, McCluskey J, Yeo JP, Tock EPC, Toh BH: EEA1, an early endosome-associated protein – EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin- binding IQ motif. J Biol Chem. 1995, 270: 13503-13511.View ArticlePubMedGoogle Scholar
- Provance DW, Wei M, Ipe V, Mercer JA: Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution. Proc Natl Acad Sci USA. 1996, 93: 14554-14558.PubMed CentralView ArticlePubMedGoogle Scholar
- Rogers SL, Gelfand VI: Myosin cooperates with microtubule motors during organelle transport in melanophores. Curr Biol. 1998, 8: 161-164.View ArticlePubMedGoogle Scholar
- Tuma MC, Gelfand VI: Molecular mechanisms of pigment transport in melanophores. Pigment Cell Res. 1999, 12: 283-294.View ArticlePubMedGoogle Scholar
- Wu X, Bowers B, Rao K, Wei Q, Hammer JA: Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo. J Cell Biol. 1998, 143: 1899-1918.PubMed CentralView ArticlePubMedGoogle Scholar
- Bridgman PC: Myosin Va movements in normal and dilute-lethal axons provide support for a dual filament motor complex. J Cell Biol. 1999, 146: 1045-1060.PubMed CentralView ArticlePubMedGoogle Scholar
- Al-Haddad A, Shonn MA, Redlich B, Blocker A, Burkhardt JK, Yu H, Hammer JA, Weiss DG, Steffen W, Griffiths G, Kuznetsov SA: Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility. Mol Biol Cell. 2001, 12: 2742-2755.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.