S-Nitrosothiols modulate G protein-coupled receptor signaling in a reversible and highly receptor-specific manner
© Kokkola et al; licensee BioMed Central Ltd. 2005
Received: 05 November 2004
Accepted: 25 April 2005
Published: 25 April 2005
Recent studies indicate that the G protein-coupled receptor (GPCR) signaling machinery can serve as a direct target of reactive oxygen species, including nitric oxide (NO) and S-nitrosothiols (RSNOs). To gain a broader view into the way that receptor-dependent G protein activation – an early step in signal transduction – might be affected by RSNOs, we have studied several receptors coupling to the Gi family of G proteins in their native cellular environment using the powerful functional approach of [35S]GTPγS autoradiography with brain cryostat sections in combination with classical G protein activation assays.
We demonstrate that RSNOs, like S-nitrosoglutathione (GSNO) and S-nitrosocysteine (CysNO), can modulate GPCR signaling via reversible, thiol-sensitive mechanisms probably involving S-nitrosylation. RSNOs are capable of very targeted regulation, as they potentiate the signaling of some receptors (exemplified by the M2/M4 muscarinic cholinergic receptors), inhibit others (P2Y12 purinergic, LPA1lysophosphatidic acid, and cannabinoid CB1 receptors), but may only marginally affect signaling of others, such as adenosine A1, μ-opioid, and opiate related receptors. Amplification of M2/M4 muscarinic responses is explained by an accelerated rate of guanine nucleotide exchange, as well as an increased number of high-affinity [35S]GTPγS binding sites available for the agonist-activated receptor. GSNO amplified human M4 receptor signaling also under heterologous expression in CHO cells, but the effect diminished with increasing constitutive receptor activity. RSNOs markedly inhibited P2Y12 receptor signaling in native tissues (rat brain and human platelets), but failed to affect human P2Y12 receptor signaling under heterologous expression in CHO cells, indicating that the native cellular signaling partners, rather than the P2Y12 receptor protein, act as a molecular target for this action.
These in vitro studies show for the first time in a broader general context that RSNOs are capable of modulating GPCR signaling in a reversible and highly receptor-specific manner. Given that the enzymatic machinery responsible for endogenous NO production is located in close proximity with the GPCR signaling complex, especially with that for several receptors whose signaling is shown here to be modulated by exogenous RSNOs, our data suggest that GPCR signaling in vivo is likely to be subject to substantial, and highly receptor-specific modulation by NO-derived RSNOs.
G protein-coupled receptors (GPCRs) represent the largest group of integral membrane proteins involved in signal transduction and are the most important targets of clinically marketed drugs [1–3]. The known GPCRs mediate messages from ligands as diverse as neurotransmitters, lipid mediators, hormones, and sensory stimuli . The classical scheme of GPCR signaling implies that agonist-induced conformational changes in receptor molecule will result in activation of cognate G proteins and subsequently in the regulation of downstream effectors, second messengers, and the activation of protein kinases, for example . However, recent work has indicated that GPCR signaling is subject to complex, cell-type specific regulation, involving a plethora of kinases, as well as newly-identified signaling partners, such as regulators of G protein signaling (RGS) , and activators of G protein signaling (AGS) .
Nitric oxide (NO) is a unique gaseous messenger generated in vivo by three isoforms of NO synthases (NOS). The established mode of NO signaling is through the activation of the hemoprotein, soluble guanylyl cyclase, resulting in increased production of the second messenger cGMP. However, accumulating evidence points towards cGMP-independent mechanisms by which NO can react with proteins, forming covalent post-translational modifications . S-nitrosothiols (RSNOs) are biological metabolites of NO, that may prolong and spatially extend the in vivo actions of locally produced NO . NO and RSNOs can reversibly react with free SH-groups of target cysteine (Cys) residues, including those in proteins, leading to S-nitrosylation and/or S-thiolation (disulfide linkage of low-molecular weight thiols to proteins) [8–17]. A broad functional spectrum of potential S-nitrosylation target proteins is currently recognized. A growing list of targets include ion channels, transporters, transcription factors, signaling proteins, metabolic enzymes, as well as respiratory proteins [7, 14, 18–20]. Although individual components of the GPCR signaling machinery are implicated as potential targets of reactive oxygen species (ROS), including NO [21–35], a broader view on how NO, and RSNOs in particular, might modulate GPCR signaling, has not been established.
To begin to address these issues, we have studied how exogenous RSNOs affect receptor-mediated G protein activity – a very proximal step of GPCR signal transduction – by studying the signaling of several receptors that couple to the Gi family of heterotrimeric G proteins. This family consists of both pertussis toxin sensitive (Gαi1-3, Gαo, transducin, gustducin) and insensitive (Gαz) members. We applied the powerful functional approach of [35S]GTPγS autoradiography in brain cryostat sections, as this technique allows selective detection of receptor-dependent G protein activity simultaneously in multiple brain regions with minimal disturbance of the GPCR microenvironment . We anticipated that accessibility of target thiols would be minimally disturbed in cryostat sections. Moreover, it is increasingly recognized that specialized plasma membrane microdomains (variously described as detergent-resistant fractions, low-density fractions, lipid rafts, or caveolae) act as unique signaling platforms with specific enrichment of GPCRs, their cognate G proteins, as well as effectors [37–40]. Such an enrichment is thought to be well-preserved in cryostat sections, but might be compromised to a variable extent , or even lost in bulk membrane preparations obtained using traditional protocols. To complement the autoradiography approach, we used membrane and lysate [35S]GTPγS binding assays to more systematically study the effects of RSNOs on a panel of Gi-coupled receptors in native tissues. Signaling of selected receptors was further studied after their heterologous expression in Chinese hamster ovary (CHO) cells. Our studies reveal highly receptor-specific modulation of GPCR signaling by RSNOs, as signaling of some receptors can be amplified, or inhibited, whereas for others, the activity is only marginally affected by comparable treatments. The GPCR itself and/or its native signaling partners seem to act as potential targets of RSNO action, and therefore their modulation may be diminished, or even totally masked under heterologous expression.
Exogenous RSNOs modulate GPCR signaling via mechanisms likely involving S-nitrosylation
GSNO is present in significant amounts (~15 pmol/mg protein) in the brain tissue and it is thought to act as a physiological carrier of NO for S-nitrosylation reactions [46, 47]. As shown in Figure 2 and Supplementary Figure 1 [see additional file 1], GSNO modulated GPCR responses in a dose-dependent manner, being effective at the submillimolar concentration range. However, the threshold and maximal concentrations needed for the modulation of distinct receptors slightly varied. Potentiation of M2/M4 receptor signaling was evident already with 0.05 mM GSNO, but statistically significant responses were obtained using 0.2 – 1 mM GSNO (Supplementary Figure 1 [see additional file 1]). P2Y12 receptor responses were inhibited with 0.2 mM GSNO and higher concentrations (Figure 2, Supplementary Figure 1 [see additional file 1]). LPA1 receptor signaling was only marginally affected with 0.05 mM GSNO, but was severely blunted with 0.2 mM GSNO and higher concentrations (Figure 2, Supplementary Figure 1 [see additional file 1]). Collectively, these experiments demonstrate that GSNO, a physiologically relevant RSNO, can modulate GPCR signaling in discrete brain regions in a receptor-specific and fully reversible manner.
Effects of GSNO treatment on agonist dose-response parameters in [35S]GTPγS binding assays of various Gi-coupled receptors in their native cellular environment. Membranes or lysates were preincubated in control conditions or in the presence of 0.5 mM GSNO for 30 min. Values are mean ± SE from three to four independent experiments performed in duplicate. Emax is expressed in percentage over basal with nonspecific binding subtracted.
Rat forebrain membranes
M2/M4 mAChRs (CCh)
-4.94 ± 0.05
176 ± 2
-5.55 ± 0.11**
207 ± 4**
-6.84 ± 0.26
140 ± 7
-6.53 ± 0.40
116 ± 3*
Cannabinoid CB1 (CP55940)
-7.84 ± 0.07
223 ± 3
-7.69 ± 0.08
183 ± 2***
Adenosine A1 (2ClAdo)
-6.90 ± 0.04
256 ± 3
-6.87 ± 0.12
234 ± 7*
-6.85 ± 0.09
167 ± 3
-7.31 ± 0.19
164 ± 5
-8.66 ± 0.12
173 ± 3
-8.78 ± 0.10
161 ± 2*
CHO cell lysates
-7.37 ± 0.11
202 ± 4
-7.05 ± 0.12
176 ± 4**
Human platelet membranes
-8.19 ± 0.03
279 ± 2
-8.30 ± 0.08
195 ± 3***
-5.33 ± 0.08
161 ± 2
-5.49 ± 0.09
182 ± 3**
Previous studies have suggested that RSNOs can act as NO+, NO., and NO- donors under physiological conditions . The RSNO effects on GPCR responses were not mimicked by the NO donor sodium nitroprusside (SNP), when tested at similar (0.5 mM) concentrations (Figure 3, Supplementary Table 1 [see additional file 1]). Furthermore, the NO+-releasing NO donor, nitrosodium tetrafluoroborate (NOBF4, 0.5 mM) (data not shown), hydrogen peroxide (1 mM H2O2 + 0.2 mM FeSO4) (data not shown), or the cyclic GMP analog 8-bromo-cyclic GMP (0.25 mM) (Figure 3, Supplementary Table 1 [see additional file 1]) were largely ineffective. For selected brain regions, quantitative autoradiography data on CysNO, SNP and 8-bromo-cyclic GMP are presented in the Supplementary Table 1 [see additional file 1].
RSNOs modulate GPCR signaling in native tissues in a highly receptor-specific manner
Effects of GSNO treatment on agonist (CCh) dose-response parameters in [35S]GTPγS binding assays of hM4 cell line membranes. Membranes were preincubated in control conditions or in the presence of 0.5 mM GSNO for 30 min. Values are mean ± SE from three to four independent experiments performed in duplicate. Emax is expressed in percentage over basal with nonspecific binding subtracted.
CHO cell line
-4.87 ± 0.14
586 ± 31
-4.94 ± 0.09
674 ± 23
-4.87 ± 0.05
213 ± 3
-4.98 ± 0.13
276 ± 10**
-5.21 ± 0.20
134 ± 3
-5.27 ± 0.20
154 ± 8*
-5.13 ± 0.10
198 ± 4
-5.12 ± 0.11
235 ± 6**
CHO cells express endogenous Gi-coupled LPA receptors [48–50], and similarly to the situation in brain membranes and cryostat sections, GSNO significantly inhibited LPA signaling in CHO cell lysates without affecting the agonist potency (Table 1). Platelets offer another readily accessible model to study P2Y12-Gi (specifically Gαi2) signaling in native cellular environments [42, 51, 52]. In human platelet membranes, GSNO inhibited basal G protein activity by 22 ± 4 % (mean ± SE, n = 3). This effect was statistically significant. Similar to the situation with brain P2Y12 receptor signaling, GSNO inhibited P2Y12 receptor-dependent G protein activity in human platelet membranes, but had no effect on the agonist potency (Table 1). To examine whether the receptor protein serves as a direct target of this action, human P2Y12 receptor was stably transfected into CHO cells. Several cell lines responding to 2MeSADP in [35S]GTPγS binding assays were established (our unpublished observations). However, neither GSNO nor CysNO affected the 2MeSADP dose-response curves of any of the hP2Y12-expressing cell lines (Figure 5 and data not shown). These data rule out the P2Y12 receptor protein as a direct target of the RSNO action.
The inhibitory effect of GSNO on basal and P2Y12 receptor-dependent G protein activity was not due to a nonspecific action on platelet membranes, since the signaling of another Gi-linked platelet receptor, the α2A-adrenoceptor, was significantly amplified in GSNO-treated membranes (Table 1). This effect was not restricted to platelets, nor was it unique to the α2A-subtype, as signaling of the three human α2-adrenoceptor subtypes (α2A, α2B, and α2C) was potentiated by GSNO in CHO cell lines stably expressing these receptors (our unpublished observations).
RSNOs amplify muscarinic responses by increasing the rate of GDP/GTP exchange and the number of high-affinity GTP binding sites
The amplification of M2/M4 responses by RSNOs was clearly evident in native brain tissue. Further experiments were designed to address the mechanism of this action. Results of these studies are presented in Figures 6 and 7. A time-response study on basal and CCh-stimulated [35S]GTPγS binding responses in forebrain membranes revealed that GSNO accelerated the rate of [35S]GTPγS binding in agonist-stimulated conditions (Figure 6). In contrast, GSNO had no effect on basal guanine nucleotide exchange. Given that GDP release is generally thought to be the rate-limiting step in receptor-driven G protein activation, these data indicate that the amplifying effect of GSNO on M2/M4 receptor-stimulated G protein activity is due to an accelerated rate of GDP/GTP exchange at the receptor-activated G protein α subunits. In line with this, GSNO significantly increased the number of high-affinity [35S]GTPγS binding sites available for M2/M4 receptor activation under agonist-stimulated conditions (Figure 7) (Mean ± SE: CCh-control 1.49 ± 0.02 vs. CCh-GSNO 1.75 ± 0.02 pmol/mg protein, P < 0.001, unpaired T-test). In contrast, there were no statistical differences in the potency of GTPγS to displace [35S]GTPγS in any of the tested conditions (Figure 7) [log (EC50) ± SE: basal-control -8.10 ± 0.07; basal-GSNO -8.11 ± 0.12; CCh-control -8.06 ± 0.06; CCh-GSNO -8.10 ± 0.04). These experiments indicate that RSNOs amplify muscarinic receptor-stimulated G protein activity in native brain tissue by accelerating the rate by which agonist-occupied receptors can activate their cognate G proteins.
RSNO amplification of M4 receptor responses are preserved under heterologous expression but the amplification is lost with constitutive receptor activity
The final experiments were intended to clarify whether cysteine 133 (C133), located adjacent to the G protein-interacting DRY-motif in the intracellular end of transmembrane helix 3 of the Gi-coupled muscarinic receptors (M2/M4), could serve as the molecular target of RSNO action. To this end, C133 was mutated into serine to reveal if the C133S mutation would abolish the effect of GSNO on the efficacy of CCh. However, GSNO treatment significantly increased Emax also in the mutant C133S-hM4 cell line (Figure 8, bottom panel right, Table 2), indicating that cysteine C133 of the hM4 receptor is not the specific target of RSNOs. The mutation did not affect the potency of CCh to activate G proteins via the hM4 receptor (Table 2).
[35S]GTPγS autoradiography of brain cryostat sections revealed a highly receptor-specific modulation of GPCR signaling by RSNOs in several receptor-enriched anatomical structures. This modulation was fully reversible upon addition of excess thiols. We have provided evidence indicating that S-nitrosylation, rather than other types of NO reactions or the NO – guanylyl cyclase – cGMP signaling pathway, was responsible for the observed effects. The RSNOs effects were receptor-specific, as signaling of some receptors was markedly potentiated (M2/M4 AChRs and α2-adrenoceptors), whereas that of others was clearly inhibited (P2Y12, LPA and cannabinoid CB1 receptors), while signaling of other receptors was only marginally affected (adenosine A1, MOR, and ORL1 receptors) by comparable treatments. We further demonstrated that RSNOs can amplify M2/M4 receptor responses by increasing the rate of GDP/GTP exchange as well as the number of high-affinity G protein α subunits capable of interacting with the agonist-activated receptors. The potentiating effect of RSNOs on hM4 receptor responses was preserved when this was studied in a heterologous expression system but was diminished in constitutively active hM4 receptors. We also demonstrated that the GPCR itself or its native signaling partners serve as potential targets of this modulation, as it was attenuated, or even lost, when receptor signaling was studied under heterologous expression. Our study suggests that GPCR signaling is subject to a highly receptor-specific modulation by NO-derived RSNOs.
The GPCRs and their proximal signaling partners as likely targets of RSNO action
Since [35S]GTPγS binding assays monitor G protein activation, one of the earliest measurable steps in GPCR signal transduction, it is obvious that the molecular targets of RSNO action are the receptors, their cognate G proteins and/or additional signaling partners, whose thiol modification can directly regulate guanine nucleotide binding and hence G protein activation.
It is interesting that RSNO treatment of brain sections consistently resulted in thiol-sensitive increases in basal Gi protein activity throughout the gray matter regions. However, no such effect was present in brain membrane [35S]GTPγS binding assays, nor was it detected in CHO cell membranes but in platelet membranes, RSNOs inhibited basal G protein activity by ~20%. As receptor input should be minimal in basal conditions, the differential behavior of RSNOs in cryostat sections and various membrane preparations likely reflects direct action on the Gi proteins and/or their proximal regulatory partners. It has been known for some time that G proteins can serve as direct targets of ROS, including NO [21–24, 28, 29]. Exogenous NO donors stimulate the monomeric G protein p21ras via S-nitrosylation to a single cysteine residue [22, 53]. Furthermore, Gαi and Gαo serve as direct protein targets of ROS, and they can be activated in the absence of input from the GPCRs [29, 33]. Specifically, ROS were shown to modify two cysteine residues of Gαi/o and this modification accelerated GDP release from Gα with a concomitant increase in the formation of the GTP-bound form of Gα without receptor activation [29, 33]. Furthermore, GSNO was reported to stimulate basal G protein activity in bovine aortic endothelial cells and human peripheral blood mononuclear cells [22, 26]. The RSNO-elicited increase in basal G protein activity in brain sections is consistent with these findings. However, it is not clear why no such stimulation was detected in bulk membrane preparations. One explanation could be the differential accessibility of target thiol(s) in cryostat sections as compared to membrane preparations. It is reasonable to assume that thiol accessibility in the former situation is close to natural. It is also possible that some crucial regulatory component of the signaling machinery is lost in bulk membrane preparations. Since several high-speed centrifugation steps were employed to obtain the relatively pure membrane preparation used in our study, this possibility should not be underestimated. This could also explain why RSNOs had no effect on basal G protein activity in CHO cell membranes, although several members of the Gi family, including Gαi1/2, Gαi3, and Gαo, are endogenously present in these cells .
In platelet membranes, RSNOs inhibited both basal and P2Y12 receptor-dependent G protein activity, but clearly potentiated α2A-adrenoceptor responses. Platelet P2Y12 receptors couple to Gαi2 [51, 52], whereas α2A-adrenoceptors can communicate via Gαz, at least in the mouse . Collectively these data suggest that RSNOs inhibit both basal and receptor-stimulated Gαi2 activity in the platelets. It is not yet known whether brain P2Y12 receptors couple to this particular G protein subtype. Further, it is unclear whether LPA receptors in brain and CHO cells communicate via Gαi2. It is interesting that RSNOs inhibited the signaling of all three receptors in native tissues. In addition, we found that brain cannabinoid CB1 receptor signaling was inhibited by RSNOs. A previous study reported that pulmonary vasoconstriction by serotonin was also inhibited by GSNO .
Although the basic module of GPCR signaling is traditionally considered to be the receptor, its cognate G protein, and the effector, recent studies have identified a wide range of proteins that can directly interact with the receptor and/or G proteins. These can modulate signaling efficiency, cellular localization, or the regulation of the GPCRs or G proteins . One such recently-identified protein is the brain-enriched, Ras-related monomeric G protein Dexras1 (human counterpart is termed activator of G protein signaling 1, AGS1). Dexras1/AGS1 is physiologically activated upon NMDA receptor-stimulated NO synthesis and S-nitrosylation on cysteine C11 [56, 57]. Dexras1/AGS-1 also interacts with Gαi/Gαo, and can activate GPCR signaling systems independently of receptor input [6, 58]. Interestingly, Dexras1/AGS-1 was shown to proximally antagonize the signaling of M2 AChRs and formyl peptide receptors, possibly by altering the pool of G proteins available for receptor coupling and/or disruption of a preformed signaling complex [59, 60]. It is currently unknown whether RSNOs and/or S-nitrosylation could alter the ability of Dexras1/AGS-1 to modify G protein function and/or input from the GPCRs. In light of the present findings, this should be an attractive hypothesis for future studies.
Most Gα proteins are palmitoylated at a cysteine near the amino terminus and this modification is required for G protein targeting to lipid rafts  and/or normal signaling . Addition and removal of the palmitoyl group appear to be dynamic receptor-mediated processes that may contribute to recycling of Gα between the membrane and cytosolic compartments . Since our experiments used only nonliving tissue, it is unlikely that the RSNO actions would achieve such extensive lipid modifications, although such alterations reportedly occur after exposure of living cells to NO .
Specialized plasma membrane microdomains act as unique platforms with specific enrichment of GPCRs, their signaling partners, and the enzymatic machinery for NO biosynthesis [35, 37–40]. Such close proximity of the GPCR signaling complex and NO source is particularly relevant for many of the receptors whose signaling was shown here to be reversibly modulated by RSNOs.
In the heart, endothelial NOS (eNOS) is localized n caveolin-enriched myocyte membrane fractions and it has been shown that lipid draft-disrupting agents severely compromise NO-dependent inhibition of adenylyl cyclase types 5 and 6 . M2-mediated parasympathetic cardiac signaling also critically involves eNOS activation and NO production . On the other hand, caveolar sequestration of M2 receptors and NO signaling was shown to be finely tuned in the myocytes , suggesting a dynamic interplay between the M2 receptor and NO. In hM4 expressing CHO cells, SNP induced agonist-independent internalization of the receptor via atropine- and thiol-sensitive mechanisms . Furthermore, ROS including NO, potentiate cardiac M2 receptor signaling via poorly defined mechanisms [32, 68]. Consistent with these findings, our study revealed robust amplification of M2/M4 receptor signaling by RSNOs both in the brain and in CHO cells expressing the hM4 receptor. In CHO cells, however, the RSNO effect clearly diminished with increasing constitutive receptor activity, suggesting that RSNO action and constitutive receptor activity likely share common mechanisms, including accelerated GDP/GTP exchange in cognate Gα subunits. As far as we are aware, this is the first study to show robust, and highly region-specific amplification of M2/M4 receptor signaling in discrete anatomical loci of the central nervous system. The functional consequences of these findings remain to be established.
The P2Y12 receptor plays a central role in platelet activation and aggregation . Previous studies have indicated that endothelial and platelet-derived NO, as well as exogenous RSNOs, are potent inhibitors of platelet aggregation [70–73]. Both cGMP-dependent and -independent mechanisms and several potential molecular targets have been implicated in these effects [72, 74]. A previous report provided evidence for cGMP-mediated signaling in the inhibition of platelet Gi signaling . The present study adds further dimensions to this scheme by demonstrating that RSNOs can inhibit platelet (and brain) P2Y12 receptor function via cGMP-independent mechanisms, likely involving S-nitrosylation. However, since the effect was lost in CHO cells stably expressing the hP2Y12 receptor, the native signaling partners, rather than the P2Y12 receptor, serve as obvious targets of this action.
One of the novel findings in this study was that RSNOs strongly inhibited Gi-mediated LPA receptor signaling in the brain and in CHO cells. The relevance of this finding with respect to brain LPA1 receptor signaling remains to be established. In vivo, peripheral LPA receptor signaling is closely associated with NO. In bovine aortic endothelial cells, LPA stimulates endothelial NOS via Gi-coupled LPA receptors . LPA is released from activated platelets and this stimulates other platelets to activate aggregation processes [76, 77]. Inhibition of LPA signaling via endogenous RSNOs could be one important mechanism by which endothelium-derived NO can suppress LPA-mediated athero- and thrombogenic signaling.
In conclusion, this study revealed that G protein activation, an early step of GPCR signal transduction, is subject to a reversible and highly receptor-specific modulation by exogenous RSNOs at physiologically relevant concentrations. Since NOS synthases (and thus NO production) have been shown to reside in close proximity with the GPCR signaling machinery, especially for many of the receptors whose signaling is subject to modulation by exogenous RSNOs, these findings suggest that GPCR signaling in vivo is likely to be finely tuned by NO-derived RSNO species. Future studies should aim at pinpointing the precise molecular targets of these actions, and at understanding the specific modifications (S-nitrosylation and/or S-thiolation) involved, as well as revealing the physiological and/or pathophysiological relevance in vivo.
All drugs and chemicals were from Sigma (St. Louis, MO) or Merck (Darmstadt, Germany), unless otherwise stated. Cell culture media, sera, and antibiotics were from Euroclone (Pero, Italy). Protein concentrations were determined with Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Adenosine deaminase (ADA) was purchased from Roche (Mannheim, Germany) and guanosine-5'-O-(3-[35S]-thio)-triphosphate ([35S]GTPγS; initial specific activity 1250 Ci/mmol) from NEN (Boston, MA). CP55940, DAMGO, nociceptin, and SNAP were purchased from Tocris Cookson Ltd. (Bristol, UK).
Human M4 muscarinic receptor (hM4, gift from Dr. Johnny Näsman, University of Kuopio) was subcloned from pBluescript into pcDNA3 mammalian expression vector and a triple hemagglutinin (HA) epitope tag was subcloned after the initiating Met codon of the hM4 gene. This construct was used to create a C133S mutant hM4 receptor with QuickChange Site Directed Mutagenesis Kit (Stratagene, La Jolla, CA). Human P2Y12 purinergic receptor (hP2Y12) was amplified from QuickClone human brain cDNA (Stratagene) using RT-PCR with gene-specific primers. The PCR product was ligated into pcDNA3 and a N-terminal hemagglutinin (HA) epitope tag was inserted in a PCR reaction with 5' primer containing the HA tag DNA sequence. All receptor constructs were confirmed by restriction analyses and DNA sequencing prior to transfections.
Cell culture and transfection
Recombinant plasmids were introduced into Chinese hamster ovary (CHO) cells with Lipofectamine 2000 transfection reagent (Gibco, Paisley, UK). Transfected cells were placed under G-418 selection (600 μg/ml) and several cell lines originating from single G-418 resistant cells were isolated. The G-418 resistant cell lines were cultured as monolayers with 100 μg/ml G-418 in Ham's F-12 nutrient mixture, containing 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2 / 95% air. Stable cell lines were analyzed for HA tag (and thus receptor) expression using receptor ELISA with mouse anti-HA primary antibody . The cell lines that showed high receptor expression levels in receptor ELISA were maintained for subsequent experiments. From these, several hM4 and hP2Y12 cell lines were established that responded to carbachol (CCh) or 2-methylthio-ADP (2MeSADP), respectively. Nontransfected CHO cells did not respond to CCh (data not shown). CHO cells endogenously express Gq-coupled P2Y receptors (P2Y1 and P2Y2). However, CHO cells that had not been transfected with the hP2Y12 receptor construct did not respond to 2MeSADP, indicating that activation of Gq was not detected using [35S]GTPγS binding assays (data not shown).
Preparation of cryostat sections, membranes, and cell lysates
Naïve, four-week-old male Wistar rats were used for the preparation of brain cryostat sections essentially as described earlier [41, 79]. All animal protocols were approved by the local ethics committee. Platelet membranes were prepared from expired human platelets (Red Cross, Helsinki, Finland) as previously described , except that protease inhibitor cocktail was omitted. CHO cells were harvested by scraping in PBS containing 5 mM EDTA. Membrane fractions (P2) from rat brains and hM4 cell lines were isolated with differential centrifugation using previously published protocols [80, 81]. For membrane preparation from hP2Y12-expressing cell lines, the centrifugation method used for platelet membrane preparation  was used, with the modification of omitting the protease inhibitor cocktail. For the preparation of whole-cell lysates, the cells were harvested by trypsinization in normal growth medium. Cell density was counted in a hemocytometer and the cells were pelleted by centrifugation for 10 min at 250 × g at room temperature. The cell pellets were washed with PBS and centrifuged twice as above, after which the dry pellets were snap-frozen on dry ice. The frozen pellets were thawed for 1 min in a water bath at room temperature, after which snap-freezing was repeated. The final membrane and lysate preparations (1–5 mg protein/ml) were stored as single-use aliquots in -75°C.
Preparation of S-nitrosothiols
S-nitroso-N-acetyl-D,L-penicillamine (SNAP) was purchased from Tocris Cookson Ltd. (Bristol, UK). All other RSNOs were synthesized from the respective thiols using acidified NaNO2. For example, S-nitrosoglutathione (GSNO) was prepared by mixing 100 μl sodium nitrite (100 mM) with 100 μl HCl (150 mM) and adding 100 μl reduced glutathione (100 mM). Reactions were allowed to proceed for 10 min at room temperature, protected from light. Reaction mixtures were neutralized with 150 μl NaOH (100 mM) and used immediately in the experiments. Millipore-quality water was used throughout and the assay buffer routinely contained 1 mM EDTA. The concentrations of RSNOs were determined by UV spectroscopy using previously published  values for the molar absorption coefficients (ε) and absorption maxima (λmax).
The assay was conducted under optimized conditions, where basal noise due to tonic adenosine A1 receptor activity has been eliminated . Experiments were conducted in light-protected chambers and in the absence of dithiotreitol (DTT), unless indicated otherwise. Briefly, the assay consisted of preincubation for 20 min at 20°C in buffer A (50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 100 mM NaCl, 5 mM MgCl2), followed by GDP loading and RSNO treatment for 1 h at 20°C in buffer A, containing additionally 2 mM GDP and 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 10-6 M) or adenosine deaminase (ADA, 1 U/ml) to eliminate tonic adenosine A1 receptor activity. When peptide agonists were used, protease inhibitor cocktail (Sigma P-2714) was included in step 2 at the concentrations recommended by the manufacturer. For [35S]GTPγS binding, sections were incubated for 90 min at 20°C in buffer A, containing additionally 80 pM [35S]GTPγS, 2 mM GDP, DPCPX (10-6 M) or ADA (1 U/ml), and the receptor agonists and/or reduced thiols (DTT, GSH or cysteine), as detailed in the results section. Nonspecific binding (Nsb) was determined in the presence of 10 μM GTPγS. The sections were washed twice at 0°C for 5 min in washing buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.4), rinsed in ice-cold deionized water for 30 s, air dried and apposed to Biomax™ MR film (Kodak) for 6–11 days. Autoradiography images were digitized and processed for figures, as previously described .
[35S]GTPγS membrane binding assays
The incubations were carried with slight modifications to previously published protocols [80, 81]. Membranes or lysates were preincubated for 30 min at room temperature in 50 mM Tris-HCl (pH 7.4), 1 mM EDTA, 100 mM NaCl, 5 mM MgCl2, 10 μM GDP and 0.5 U/ml ADA, under constant shaking and protected from light. RSNOs (usually GSNO or CysNO) were included in the preincubation at final concentrations of 0.5 mM. The assay was performed in duplicate in a final assay volume of 400 μl. The reaction was initiated by adding 40 μl of membrane or lysate preparation (5 μg protein or 50,000 cells / tube) to incubation tubes containing drug dilutions and binding cocktail. The final concentrations of the components in binding reaction were 50 mM Tris-HCl (pH 7.4), 1 mM EDTA, 100 mM NaCl, 5 mM MgCl2, 0.5% BSA, 10 μM GDP, 0.5 U/ml ADA and 150 pM [35S]GTPγS. Nsb was defined using 10 μM GTPγS. Reaction tubes were incubated for 90 min at 25°C under constant shaking. The reaction was quickly terminated by the addition of 4 ml ice-cold wash buffer (50 mM Tris-HCl (pH 7.4), 5 mM MgCl2) followed by rapid filtration through Whatman GF/B glass fiber filters (Whatman, Maidstone, UK) and two additional 4 ml washes with the buffer. In the time-response study (Figure 6), initial reaction volume was 2 ml and aliquots (400 μl) from duplicate samples were draw at different time points (15, 30, 60 and 90 min), and the reaction was terminated as described above. Radioactivity in filters was counted with Wallac Rackbeta liquid scintillation counter (Wallac, Turku, Finland). It should be noted that RSNO treatment caused a small, yet consistent increase in Nsb (25 ± 4 %, mean ± SEM, n = 13). However, as Nsb represented <0.3 % of total radioactivity, this effect was considered negligible and did not contribute to the results and conclusions thereof.
[35S]GTPγS-membrane binding data were analyzed with GraphPad Prism software (GraphPad, San Diego, CA) using non-linear fitting for sigmoid dose-response curves. Statistical analyses were made with one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test. When comparison was made between only two groups, unpaired T-test was used.
List of abbreviations
activator of G protein signaling
Chinese hamster ovary
[D-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin
G protein-coupled receptor(s)
human muscarinic receptor subtype 4
human P2Y12 purinergic receptor
regulator of G protein signaling
MDR is a medical student from Madrid, Spain and participated in this study as an IFMSA (International Federation of Medical Students' Association) exchange student. We wish to thank Dr. Johny Näsman (University of Kuopio) for the hM4 receptor cDNA, and Prof. Mika Scheinin (University of Turku) for the CHO cell lines expressing α2-adrenoceptor subtypes. Reija Heikkinen (M.Sc.) is acknowledged for her contribution in the generation and functional testing of hM4 cell lines. Dr. Ewen MacDonald is acknowledged for the revision of the language of this paper. We are thankful to Mrs. Taina Vihavainen, Mrs. Tiina Räsänen and Mrs. Taija Vaarala for skillful technical assistance and to Mrs. Merja Saastamoinen for secretarial help.
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