Bacillus subtilis actin-like protein MreB influences the positioning of the replication machinery and requires membrane proteins MreC/D and other actin-like proteins for proper localization
© Soufo and Graumann; licensee BioMed Central Ltd. 2005
Received: 29 November 2004
Accepted: 03 March 2005
Published: 03 March 2005
Bacterial actin-like proteins have been shown to perform essential functions in several aspects of cellular physiology. They affect cell growth, cell shape, chromosome segregation and polar localization of proteins, and localize as helical filaments underneath the cell membrane. Bacillus subtilis MreB and Mbl have been shown to perform dynamic motor like movements within cells, extending along helical tracks in a time scale of few seconds.
In this work, we show that Bacillus subtilis MreB has a dual role, both in the formation of rod cell shape, and in chromosome segregation, however, its function in cell shape is distinct from that of MreC. Additionally, MreB is important for the localization of the replication machinery to the cell centre, which becomes aberrant soon after depletion of MreB. 3D image reconstructions suggest that frequently, MreB filaments consist of several discontinuous helical filaments with varying length. The localization of MreB was abnormal in cells with decondensed chromosomes, as well as during depletion of Mbl, MreBH and of the MreC/MreD proteins, which we show localize to the cell membrane. Thus, proper positioning of MreB filaments depends on and is affected by a variety of factors in the cell.
Our data provide genetic and cytological links between MreB and the membrane, as well as with other actin like proteins, and further supports the connection of MreB with the chromosome. The functional dependence on MreB of the localization of the replication machinery suggests that the replisome is not anchored at the cell centre, but is positioned in a dynamic manner.
Actin provides vital functions as a cytoskeletal component in eukaryotic and in prokaryotic cells. In eukaryotes, actin filaments give mechanical strength to cells in form of a dynamic cytoskeleton, and are structural fibers in muscle contraction. Additionally, actin proteins have motor like functions [1–3], most notably in cell migration through pushing of membranes. Motility receptors turn on WASP family proteins, which binds to and activate the Arp2/3 complex. The latter induces branching and growth of actin filaments . In vitro, actin filaments can deform vesicles and thus push membranes, providing the force to elongate cellular extensions such as pseudopods [4, 5]. Listeria monocytogenes cells move within macrophages through propelling by actin bundles that extend at only one pole of the bacterial cells, due to the ActA protein that is present at one cell pole and that induces rapid polymerisation of actin. Bacterial cells also possess several different actin-like proteins . MreB is essential for cell viability, its depletion leads to a defect in chromosome segregation, and ultimately to the formation of round cells, i.e. to loss of rod cell shape. Except for plasmid encoded ParM protein, which actively partitions plasmids , the true mode of action and regulation of bacterial actins is still rather unclear.
During the depletion of Bacillus subtilis MreB or of Mbl, the second actin ortholog, or of Caulobacter crescentus MreB, origin regions on the chromosomes fail to separate properly, leading to a severe (or in case of Mbl more moderate) segregation defect [8, 9], likewise to overproduction of a dominant negative mreB allele in E. coli . It is unclear, if actin proteins have a direct role, e.g. as an active segregation motor, or an indirect influence on the segregation of chromosomes. In support of an active role, MreB appears to be associated with the nucleoids, in contrast to Mbl , which is thought to be involved in the insertion of new cell wall material into the growing peptide glycan layer . Plasmid encoded E. coli ParR protein binds to a specific cis site on the duplicated plasmids, which are located close to the cell centre, and induces polymerisation of the ParM actin homolog . ParM filaments contain plasmids at their pole ward ends, so two oppositely orientated ParM filaments appear to push plasmids towards each cell pole . On the other hand, C. crescentus MreB has been shown to also affect cell shape and the localization of cell wall synthesizing proteins , and to play an important role in determining the global polarity of the cell, i.e. by affecting the localization of proteins to the cell pole . MreB and Mbl form helical filaments just underneath the cell membrane [13–16], which in B. subtilis are highly dynamic. MreB and Mbl move along helical tracks, with a speed of about 0.1 μm/s, providing potential motor like force . Actin polymerises into a two stranded right handed helix through addition of ATP-bound actin monomers. Actin movement arises through growth at the barbed end of the filament, while actin is released from the pointed end following ATP hydrolysis (a process termed treadmilling). Active pushing is thought to occur through binding of actin monomers to the tip of the filament when the object moves away, thus preventing backward movement, such that the object is driven by Brownian diffusion, with the actin filament dictating a single direction (polymerisation rachett) .
Bacterial chromosome segregation is a highly organized process, depending on several essential protein complexes. DNA polymerase localizes to the cell centre throughout most of the cell cycle . During replication, the chromosome moves through this stationary replisome, and duplicated regions are rapidly moved towards opposite cell poles , where they are bound and organized by the SMC complex . It is still unknown if the localization of the replisome involves an anchor, or which factors are involved in the central positioning.
We have investigated the role of MreB and Mbl in the positioning of the replication factory, and investigated the role of other actin like proteins and membrane proteins for the localization of MreB. We have found that B. subtilis MreC and MreD proteins localize to the cell membrane, and affect the localization of MreB, likewise to Mbl and MreBH, showing that an intricate interplay exists between actin orthologs and MreCD membrane proteins.
MreB and MreC have distinct functions in cellular growth and in control of cell shape
Depletion of MreB leads to the loss of mid cell localization of the replication machinery
The depletion of Mbl also had an effect on the positioning of the replication machinery, however, to a much milder extent compared with MreB. 3–4 doubling times after the onset of depletion, 38% of the cells contained a single, mid cell-positioned focus, and 30% two foci in each cell half (roughly at the quarter positions), whereas in 32% of the cells, the DnaX-CFP signals were more than 0.4 μm away from the cell centre (Fig. 2D). Nevertheless, it is apparent from Fig. 3B that although the scatter of DnaX-CFP around the cell centre (and around the quarter positions) is larger in Mbl depleted cells compared with wild type cells, the replication machinery is still largely retained close to the cell centre, contrarily to MreB depleted cells. These results show that directly or indirectly, MreB has a major effect on the positioning of the replisome.
MreB appears to form several discontinuous helices within each cell
The localization of MreB is affected by the state of the nucleoids
It has been shown that MreB is closely associated with DNA, because no helical filaments are visible in anucleate cells, in contrast to Mbl or MreBH filaments that are found in anucleate cells . However, MreB filaments were present in cells containing nucleoids during depletion of Topo IV, which leads to a block in full separation of the chromosomes. To investigate, if MreB filaments might be affected by the shape of the nucleoids, we moved the GFP-MreB fusion into spo0J mutant cells, which have slightly decondensed DNA, or into smc mutant cells, in which the nucleoids are highly decondensed, and which contain less negatively supercoiled DNA compared to wild type cells . Wild type cells contained different numbers of distinct MreB filaments at cellular positions that also contained DNA, but not close to the cell poles, which are devoid of DNA (Fig. 2E). Contrarily, MreB formed somewhat abnormal long filaments in spo0J mutant cells (data not shown), and highly aberrant elongated filaments throughout smc mutant cells (Fig. 2F), that is the filaments extended right to the cell poles, had fewer gaps than in wild type cells, and the spacing between individual turns was much shorter compared with wild type cells. These findings indicate that the formation of proper MreB filaments is influenced by the state of the chromosomes. In agreement with earlier results, GFP-MreB filaments were not observed in all of the 35 anucleate smc mutant cells monitored (forming about 15% anucleate cells ).
Formation of MreB filaments is influenced by MreC and MreD membrane proteins, and by other actin proteins
MreB is upstream of mreC and mreD genes, whose depletion leads to formation of round cells (see above, [8, 22, 26]). Both gene products are highly hydrophobic, and MreD is predicted to form at least 5 membrane spanning helices (data not shown). N-terminal GFP fusions to both proteins were fully functional, and showed a uniform staining of the cell membrane (Fig. 2G and 2H). Thus, both proteins are associated with the cell membrane. Lee and Stewart have used immuno-gold labelling to show that MreC is predominantly found at the septum between cells . It is clear from Fig. 2G and 2H that MreC and MreD fluorescence is highest at the septum, because two membranes are closely adjacent to each other, which is most likely the explanation for why immuno-gold labels were enriched at this location.
As opposed to depletion of MreC or MreD, depletion of Mbl or of MreBH leads to a high number of cells, in which MreB filaments extended throughout the entire cell (about 40% of the cells 2 doubling times after depletion of Mbl or of MreBH, indicated by grey arrows, Fig. 5D and 5F), or in which only foci were visible (25%, white arrow, Fig. 5F). In Mbl depleted cells (which are bulgy and twisted [8, 14, 27]), only highly aberrant and weak GFP-MreB filaments were detectable (Fig. 5E), while the more vibrio-shaped MreBH depleted cells contained highly irregular MreB filaments (Fig. 5G). Thus, formation of proper MreB filaments is affected by Mbl and MreBH. However, even the highly abnormal MreB filaments in Mbl depleted cells are able to support cell viability, albeit at a highly reduced level (mbl deleted cells grow extremely slowly [14, 27]).
This work provides several important conclusions on the function and localization of the B. subtilis actin ortholog MreB. Our experiments establish that MreB has a dual function, it is vital for the formation of proper rod shape of the cells, and for regular chromosome segregation. However, its function in cell shape is different from that of MreC, or of MreD. The depletion of MreC and MreD leads to rapid cessation of growth and to the formation of small round cells, whereas the sole depletion of MreB results in the formation of large oval shaped cells, and a slower occurring growth arrest. Interestingly, though, we found a connection between MreC and MreB, because during depletion of MreCD, MreB formed fewer and usually abnormally shaped helical filaments. Similar observations have recently been made in E. coli cells . Our experiments show that MreC and MreD localize throughout the B. subtilis membrane, establishing a link between MreB and the membrane. We speculate that MreC and MreD might provide low affinity binding sites for MreB, such that the filaments extend underneath the membrane in a regular helical pattern. Our results also suggest a dual function for MreC, because its deletion affects the localization of MreB (which is apparently not severe enough to strongly interfere with chromosome segregation), as well as cell shape (in a manner distinct from MreB).
An important, if not crucial function of MreB is the positioning of the replication machinery in B. subtilis cells. Soon after the depletion of MreB, the replisome lost its central position in the cell, before a change in cell shape was apparent. The depletion of Mbl had only a minor effect on the localization of the replisome, showing that MreB also affects the positioning of an intracellular protein assembly. Our results do not distinguish between the possibilities that the lack of MreB activity results in the loss of central localization of the replisome, which in turn leads to a segregation defect, or that a more direct defect in chromosome segregation due to the lack of MreB might cause mislocalization of the replisome. However, it is tempting to speculate that MreB could actively push DNA away from the central replisome towards opposite cell poles, and that the net result of this simultaneous pushing of ejected DNA towards opposite directions might lead to a balanced positioning of the replisome towards the cell centre, without any need for an anchor. This is in agreement with recent data showing that the replication machinery is highly mobile around the cell centre [29, 30].
An intriguing property of bacterial actin orthologs is the formation of highly dynamic helical filaments underneath the cell membrane that for some members of this protein family are thought to extend through the entire cell length [14, 16]. Three dimensional image reconstructions have helped to resolve the nature of the helical MreB filaments in live cells. MreB does not form a closed cytoskeleton like structure, but different forms of filaments within a single cell. These filaments can stretch along a half turn up to a full turn underneath the membrane, but are not clearly connected with each other. This is in agreement with findings showing that several MreB filaments move continuously along helical tracks , generating motor-like intracellular movement.
We also provide evidence that the formation of MreB filaments is affected by the nature of the nucleoids, and by the other actin like proteins. In smc mutant cells, MreB filaments are abnormally spaced and extended, and to a much lesser extent in spo0J mutant cells. This further supports and extends our earlier findings that a connection exists between MreB and the nucleoids. Interestingly, smc mutant cells are elongated and frequently twisted and wider than wild type cells , which might be due to the effect on MreB. Likewise, the depletion of Mbl or of MreBH interfered with formation of proper MreB filaments, revealing a tight link between the three actin orthologs. It will be interesting to investigate how these proteins localize relative to each other within a single cell, and if they even physically interact with each other.
Our findings show that an intricate interplay exists between MreB, membrane associated MreC and MreD proteins, other actin orthologs, the replication machinery and the nucleoids, shedding light on the question why the depletion of MreB affects both, chromosome segregation and cell shape. What remains to be investigated are several important questions, e.g. what is the mode of interaction between MreB and the MreCD proteins or with Mbl and MreBH, and to identify the link between MreB and the nucleoids or the replisome, to distinguish between the causality of defects caused by the loss of MreB activity. Also, it will be highly revealing to identify the possible load MreB might be pushing, if its dynamic movement indeed constitutes a motor function within the prokaryotic cell.
Escherichia coli XL1-Blue (Stratagene) or B. subtilis strains were grown in Luria-Bertani (LB) rich medium supplemented with 50 μg/ml ampicillin or other antibiotics, where appropriate. For induction of the hyperspank promoter, the culture media were supplemented with 0.1 to 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG). For induction of xylose promotor, glucose in S750 medium was exchanged for 0.5% fructose and xylose was added up to 0.5%.
Constructions of plasmids
Gfp mut1 including MCS was amplified from pSG1729  and was cloned into pSG1164  in which the gfp mut1 for C-terminal fusion had been excised using Kpn I and Spe I. The resulting plasmid pHJDS1 was used to generate N-terminal GFP fusions at the original gene locus. To obtain inducible N-terminal GFP fusion alleles of mreC or of mreD at the original locus, the 5' prime regions (350 to 500 bp) of the genes were PCR amplified and inserted in the EcoR I and Apa I sites of plasmid pHJDS1 to generate pJS14 or pJS15, respectively. To create a fusion of GFP to the N-terminus of mreB, mreC or mreD at an ectopic site on the chromosome, the entire sequences of theses genes were PCR amplified and inserted into the Eco RI and Apa I sites of plasmid pSG1729  to generate pJS17, pJS19, or pJS20, respectively. To generate an IPTG inducible copy of gfp-mreB (pJS22) or of mreCD (pJS23) at the amylase locus, gfp-mreB was PCR amplified from pJS17 and mreCD from B. subtilis PY79 chromosomal DNA, and the products were cloned as Hin dIII-Sph I or as Sal I-Sph I fragments, respectively, immediately downstream of the hyperspank promotor in plasmid pDR111 (kind gift of D. Rudner, Harvard Medical School).
To express GFP-MreC or GFP-MreD at their original locus in Bacillus, pJS14 or pJS15 plasmids were transformed into wild type B. subtilis (PY79) selecting for chloramphenicol resistance (Cm, 5 μg/ml) to generate strains JS14 (Pxyl-gfp-mre C) or JS15 (Pxyl-gfp-mreD), respectively. For GFP N-terminal fusions at the amy locus, plasmids pJS19 and pJS20 for mreC and mreD were transformed into PY79 selecting for spectinomycin resistance (spec, 25 μg/ml) to generate strains JS19 (Pxyl-gfp-mreC::amy) and JS20 (Pxyl-gfp-mreD::amy), respectively. Strain JS32, in which mreB can be depleted in the presence or absence of mreCD, was created by transforming compentent JS1 cells with chromosomal DNA of JS32. To examine the subcellular localization of GFP-MreB in spo0J or in smc null cells, strain JS19 was transformed with chromosomal DNA from strains AG1468  or PGΔ388 , generating strains JS23 (Pxyl-gfp-mreB::amy, ΔspoOJ) and JS24 (Pxy-gfp-mreB::amy, smc::kan) respectively. To be able to visualize the localisation patterns of labelled MreB helices in cells depleted of MreC, MreD, Mbl and MreBH cells, chromosomal DNA from strains JS3 (Pxyl-mreC), JS4 (Pxyl-mreD), JS2 (Pxyl-mbl) and JS5 (Pxyl-mreBH) was used to transform strain JS25 (Phyperspank-gfp-mreB::amy)selecting for Cm and spec, generating strains JS29 (Phyperspank-gfp-mreB::amy, Pxyl-mreC), JS30 (Phypespank-gfp-mreB::amy, Pxyl-mreD), JS28 (Phyperspank-gfp-mreB::amy, Pxyl-mbl), and JS31 (Phyperspank-gfp-mreB::amy, Pxyl-mreBH). To express DnaX-CFP in MreB or Mbl depleted cells, chromosomal DNA from JS1 and JS2 was used to transform PG24 competent cells.
For microscopic analysis, Bacillus strains were grown in S750 defined medium  complemented with 1% casamino acids. Fluorescence microscopy was performed on an Olympus AX70 microscope. Cells were mounted on agarose gel pads containing S750 growth medium on object slides. Images were acquired with a digital CCD camera; signal intensities and cell length were measured using the Metamorph 4.6 program (Universal Imaging Corp., USA). For and 3D reconstruction, 10 to 12 images (spacing between 0.2 to 0.38 μm) were taken through the focal plane, and processed in Metamorph 6 program. DNA was stained with 4',6-diamidino- 2-phenylindole (DAPI; final concentration 0.2 ng/ml) and membranes were stained with FM4-64 (final concentration 1 nM).
We would like to thank the reviewers for their helpful comments. This work was supported through a Heisenberg fellowship of the Deutsche Forschungsgemeinschaft.
- Mogilner A, Oster G: Polymer motors: pushing out the front and pulling up the back. Curr Biol. 2003, 13: R721-733. 10.1016/j.cub.2003.08.050.View ArticlePubMed
- Upadhyaya A, van Oudenaarden A: Biomimetic systems for studying actin-based motility. Curr Biol. 2003, 13: R734-44. 10.1016/j.cub.2003.08.051.View ArticlePubMed
- Marx J: How cells step out. Science. 2003, 302: 214-216. 10.1126/science.302.5643.214.View ArticlePubMed
- Lauffenburger DA, Horwitz AF: Cell migration: a physically integrated molecular process. Cell. 1996, 84: 359–369-10.1016/S0092-8674(00)81280-5.View ArticlePubMed
- Mitchison TJ, Cramer LP: Actin-based cell motility and cell locomotion. Cell. 1996, 84: 371–379-10.1016/S0092-8674(00)81281-7.View ArticlePubMed
- van den Ent F, Amos LA, Lowe J: Prokaryotic origin of the actin cytoskeleton. Nature. 2001, 413: 39-44. 10.1038/35092500.View ArticlePubMed
- Moller-Jensen J, Borch J, Dam M, Jensen RB, Roepstorff P, Gerdes K: Bacterial Mitosis. ParM of Plasmid R1 Moves Plasmid DNA by an Actin-like Insertional Polymerization Mechanism. Mol Cell. 2003, 12: 1477-1487. 10.1016/S1097-2765(03)00451-9.View ArticlePubMed
- Defeu Soufo HJ, Graumann PL: Actin-like Proteins MreB and Mbl from Bacillus subtilis Are Required for Bipolar Positioning of Replication Origins. Curr Biol. 2003, 13: 1916-1920. 10.1016/j.cub.2003.10.024.View Article
- Gitai Z, Dye N, Shapiro L: An actin-like gene can determine cell polarity in bacteria. Proc Natl Acad Sci U S A. 2004, 101: 8643-8648. 10.1073/pnas.0402638101.PubMed CentralView ArticlePubMed
- Kruse T, Moller-Jensen J, Lobner-Olesen A, Gerdes K: Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. EMBO J. 2003, 22: 5283-5292. 10.1093/emboj/cdg504.PubMed CentralView ArticlePubMed
- Daniel RA, Errington J: Control of cell morphogenesis in bacteria: two distinct ways to make a rod-shaped cell. Cell. 2003, 113: 767-776. 10.1016/S0092-8674(03)00421-5.View ArticlePubMed
- Moller-Jensen J, Jensen RB, Lowe J, Gerdes K: Prokaryotic DNA segregation by an actin-like filament. EMBO J. 2002, 21: 3119-3127. 10.1093/emboj/cdf320.PubMed CentralView ArticlePubMed
- Figge RM, Divakaruni AV, Gober JW: MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus. Mol Microbiol. 2004, 51: 1321-1332. 10.1111/j.1365-2958.2003.03936.x.View ArticlePubMed
- Jones LJ, Carballido-Lopez R, Errington J: Control of cell shape in bacteria: helical, actin-like filaments in Bacillus subtilis. Cell. 2001, 104: 913-922. 10.1016/S0092-8674(01)00287-2.View ArticlePubMed
- Carballido-Lopez R, Errington J: The bacterial cytoskeleton: in vivo dynamics of the actin-like protein Mbl of Bacillus subtilis. Dev Cell. 2003, 4: 19-28. 10.1016/S1534-5807(02)00403-3.View ArticlePubMed
- Shih YL, Le T, Rothfield L: Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles. PNAS. 2003, 100: 7865-7870. 10.1073/pnas.1232225100.PubMed CentralView ArticlePubMed
- Defeu Soufo HJ, Graumann PL: Dynamic movement of actin-like proteins within bacterial cells. EMBO Reports. 2004, 5: 789-794. 10.1038/sj.embor.7400209.PubMed CentralView ArticlePubMed
- Lemon KP, Grossman AD: Movement of Replicating DNA through a Stationary Replisome. Mol Cell. 2000, 6: 1321-1330. 10.1016/S1097-2765(00)00130-1.View ArticlePubMed
- Wu LJ: Structure and segregation of the bacterial nucleoid. Curr Opin Genet Dev. 2004, 14: 126-132. 10.1016/j.gde.2004.01.006.View ArticlePubMed
- Volkov AV, Mascarenhas J, Andrei-Selmer C, Ulrich HD, Grauman PL: A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid. Mol Cell Biol. 2003, 23: 5638-5650. 10.1128/MCB.23.16.5638-5650.2003.PubMed CentralView ArticlePubMed
- Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, Boland F, Brignell SC, Bron S, Bunai K, Chapuis J, Christiansen LC, Danchin A, Debarbouille M, Dervyn E, Deuerling E, Devine K, Devine SK, Dreesen O, Errington J, Fillinger S, Foster SJ, Fujita Y, Galizzi A, Gardan R, Eschevins C, Fukushima T, Haga K, Harwood CR, Hecker M, Hosoya D, Hullo MF, Kakeshita H, Karamata D, Kasahara Y, Kawamura F, Koga K, Koski P, Kuwana R, Imamura D, Ishimaru M, Ishikawa S, Ishio I, Le Coq D, Masson A, Mauel C, Meima R, Mellado RP, Moir A, Moriya S, Nagakawa E, Nanamiya H, Nakai S, Nygaard P, Ogura M, Ohanan T, O'Reilly M, O'Rourke M, Pragai Z, Pooley HM, Rapoport G, Rawlins JP, Rivas LA, Rivolta C, Sadaie A, Sadaie Y, Sarvas M, Sato T, Saxild HH, Scanlan E, Schumann W, Seegers JF, Sekiguchi J, Sekowska A, Seror SJ, Simon M, Stragier P, Studer R, Takamatsu H, Tanaka T, Takeuchi M, Thomaides HB, Vagner V, van Dijl JM, Watabe K, Wipat A, Yamamoto H, Yamamoto M, Yamamoto Y, Yamane K, Yata K, Yoshida K, Yoshikawa H, Zuber U, Ogasawara N: Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A. 2003, 100: 4678-4683. 10.1073/pnas.0730515100.PubMed CentralView ArticlePubMed
- Lee JC, Stewart GC: Essential nature of the mreC determinant of Bacillus subtilis. J Bacteriol. 2003, 185: 4490-4498. 10.1128/JB.185.15.4490-4498.2003.PubMed CentralView ArticlePubMed
- Lemon KP, Grossman AD: Localization of bacterial DNA polymerase: evidence for a factory model of replication. Science. 1998, 282: 1516-1519. 10.1126/science.282.5393.1516.View ArticlePubMed
- Lindow JC, Britton RA, Grossman AD: Structural maintenance of chromosomes protein of Bacillus subtilis affects supercoiling in vivo. J Bacteriol. 2002, 184: 5317-5322. 10.1128/JB.184.19.5317-5322.2002.PubMed CentralView ArticlePubMed
- Graumann PL, Losick R, Strunnikov AV: Subcellular localization of Bacillus subtilis SMC, a protein involved in chromosome condensation and segregation. J Bacteriol. 1998, 180: 5749-5755.PubMed CentralPubMed
- Wachi M, Doi M, Okada Y, Matsuhashi M: New I genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells. J Bacteriol. 1989, 171: 6511-6516.PubMed CentralPubMed
- Abhayawardhane Y, Stewart GC: Bacillus subtilis possesses a second determinant with extensive sequence similarity to the Escherichia coli mreB morphogene. J Bacteriol. 1995, 177: 765-773.PubMed CentralPubMed
- Kruse T, Bork-Jensen J, Gerdes K: The morphogenetic MreBCD proteins of Escherichia coli form an essential membrane-bound complex. Mol Microbiol. 2005, 55: 78-89. 10.1111/j.1365-2958.2004.04367.x.View ArticlePubMed
- Migocki MD, Lewis PJ, Wake RG, Harry EJ: The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events. Mol Microbiol. 2004, 54: 452-463. 10.1111/j.1365-2958.2004.04267.x.View ArticlePubMed
- Wang JD, Rokop ME, Barker MM, Hanson NR, Grossman AD: Multicopy plasmids affect replisome positioning in Bacillus subtilis. J Bacteriol. 2004, 186: 7084-7090. 10.1128/JB.186.21.7084-7090.2004.PubMed CentralView ArticlePubMed
- Mascarenhas J, Soppa J, Strunnikov A, Graumann PL: Cell cycle dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein. EMBO J. 2002, 21: 3108-3118. 10.1093/emboj/cdf314.PubMed CentralView ArticlePubMed
- Lewis PJ, Marston AL: GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene. 1999, 227: 101-110. 10.1016/S0378-1119(98)00580-0.View ArticlePubMed
- Ireton K, Gunther AD, Grossman AD: spo0J is required for normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis. J Bacteriol. 1994, 176: 5320-5329.PubMed CentralPubMed
- Jaacks KJ, Healy J, Losick R, Grossman AD: Identification and characterization of genes controlled by the sporulation regulatory gene spo0H in Bacillus subtilis. J Bacteriol. 1989, 171: 4121-4129.PubMed CentralPubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.